Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_J24
(865 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 135 3e-32
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 96 2e-20
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 96 2e-20
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 94 1e-19
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 69 3e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 40 0.002
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 40 0.002
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 35 0.061
At5g13650.2 68418.m01585 elongation factor family protein contai... 33 0.32
At5g13650.1 68418.m01584 elongation factor family protein contai... 33 0.32
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 29 4.0
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 135 bits (327), Expect = 3e-32
Identities = 88/198 (44%), Positives = 115/198 (58%), Gaps = 10/198 (5%)
Frame = -2
Query: 780 PXXLGDXSXGVQYLXEIKXSFVXXXQ-----GPXRKE----LWLKRIXVVLDSTSM-M*H 631
P + D GVQYL EIK S V Q GP +E + + VVL S ++
Sbjct: 644 PNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEENMRGICFEVCDVVLHSDAIHRGG 703
Query: 630 PILMPSIEVVAKSFQQXEDACTHVC*LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRG 451
++P+ V + Q T LL PV V+ PE A+GGIY VLN++RG
Sbjct: 704 GQVIPTARRVIYASQ-----ITAKPRLLEPVY-----MVEIQAPEGALGGIYSVLNQKRG 753
Query: 450 HVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDH*QVLPGDPCEPQ 271
HVFEE Q GTP++ +KAYLPV ESFGF++ LR+ T GQAFPQCVFDH +++ DP EP
Sbjct: 754 HVFEEMQRPGTPLYNIKAYLPVVESFGFSSQLRAATSGQAFPQCVFDHWEMMSSDPLEPG 813
Query: 270 SKPYNVVQETRKRKGLKE 217
++ +V + RKRKGLKE
Sbjct: 814 TQASVLVADIRKRKGLKE 831
Score = 95.9 bits (228), Expect = 3e-20
Identities = 42/72 (58%), Positives = 54/72 (75%)
Frame = -1
Query: 700 AXKEGVMAEENLXGVRFNIYDVTPHTDAIHRGGGQIIPTXRRCLYACLLTAQPRXMEPVY 521
A KEG +AEEN+ G+ F + DV H+DAIHRGGGQ+IPT RR +YA +TA+PR +EPVY
Sbjct: 671 ASKEGPLAEENMRGICFEVCDVVLHSDAIHRGGGQVIPTARRVIYASQITAKPRLLEPVY 730
Query: 520 LCEIQCP*SSCG 485
+ EIQ P + G
Sbjct: 731 MVEIQAPEGALG 742
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 96.3 bits (229), Expect = 2e-20
Identities = 55/126 (43%), Positives = 73/126 (57%), Gaps = 12/126 (9%)
Frame = -2
Query: 552 LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 373
+ +P L +V+ P V IY VL+RRRGHV + GTP +IVKA+LPV ESF
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 372 GFTADLRSNTGGQAFPQCVFDH*QVLPGDPCEP--QSKPY----------NVVQETRKRK 229
GF DLR +T GQAF VFDH ++PGDP + Q +P + +TR+RK
Sbjct: 896 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRK 955
Query: 228 GLKEDL 211
G+ ED+
Sbjct: 956 GMSEDV 961
Score = 68.9 bits (161), Expect = 4e-12
Identities = 31/65 (47%), Positives = 40/65 (61%)
Frame = -1
Query: 694 KEGVMAEENLXGVRFNIYDVTPHTDAIHRGGGQIIPTXRRCLYACLLTAQPRXMEPVYLC 515
+EG + +E + V+F I D + +HRG GQ+IPT RR Y+ L A PR MEPVY
Sbjct: 789 REGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYV 848
Query: 514 EIQCP 500
EIQ P
Sbjct: 849 EIQTP 853
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 96.3 bits (229), Expect = 2e-20
Identities = 55/126 (43%), Positives = 73/126 (57%), Gaps = 12/126 (9%)
Frame = -2
Query: 552 LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 373
+ +P L +V+ P V IY VL+RRRGHV + GTP +IVKA+LPV ESF
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 372 GFTADLRSNTGGQAFPQCVFDH*QVLPGDPCEP--QSKPY----------NVVQETRKRK 229
GF DLR +T GQAF VFDH ++PGDP + Q +P + +TR+RK
Sbjct: 896 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRK 955
Query: 228 GLKEDL 211
G+ ED+
Sbjct: 956 GMSEDV 961
Score = 68.9 bits (161), Expect = 4e-12
Identities = 31/65 (47%), Positives = 40/65 (61%)
Frame = -1
Query: 694 KEGVMAEENLXGVRFNIYDVTPHTDAIHRGGGQIIPTXRRCLYACLLTAQPRXMEPVYLC 515
+EG + +E + V+F I D + +HRG GQ+IPT RR Y+ L A PR MEPVY
Sbjct: 789 REGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYV 848
Query: 514 EIQCP 500
EIQ P
Sbjct: 849 EIQTP 853
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 93.9 bits (223), Expect = 1e-19
Identities = 54/126 (42%), Positives = 73/126 (57%), Gaps = 12/126 (9%)
Frame = -2
Query: 552 LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 373
+ +P L +V+ P V IY VL+RRRG+V + GTP +IVKA+LPV ESF
Sbjct: 822 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESF 881
Query: 372 GFTADLRSNTGGQAFPQCVFDH*QVLPGDPCEP--QSKPY----------NVVQETRKRK 229
GF DLR +T GQAF VFDH ++PGDP + Q +P + +TR+RK
Sbjct: 882 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRK 941
Query: 228 GLKEDL 211
G+ ED+
Sbjct: 942 GMSEDV 947
Score = 68.9 bits (161), Expect = 4e-12
Identities = 31/65 (47%), Positives = 40/65 (61%)
Frame = -1
Query: 694 KEGVMAEENLXGVRFNIYDVTPHTDAIHRGGGQIIPTXRRCLYACLLTAQPRXMEPVYLC 515
+EG + +E + V+F I D + +HRG GQ+IPT RR Y+ L A PR MEPVY
Sbjct: 775 REGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYV 834
Query: 514 EIQCP 500
EIQ P
Sbjct: 835 EIQTP 839
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 69.3 bits (162), Expect = 3e-12
Identities = 35/99 (35%), Positives = 52/99 (52%)
Frame = -2
Query: 579 EDACTHVC*LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 400
+DAC +P + F + + +G +Y VL+RRR + +E G+ +F V
Sbjct: 860 KDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTVH 919
Query: 399 AYLPVNESFGFTADLRSNTGGQAFPQCVFDH*QVLPGDP 283
AY+PV+ESFGF +LR T G A V H ++L DP
Sbjct: 920 AYVPVSESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958
Score = 28.7 bits (61), Expect = 5.3
Identities = 20/72 (27%), Positives = 32/72 (44%), Gaps = 8/72 (11%)
Frame = -1
Query: 700 AXKEGVMAEENLXGVRFNIYD-VTP----HTDAIHRGG---GQIIPTXRRCLYACLLTAQ 545
A G + +E + G+ F I + P TD G GQ++ + A +L
Sbjct: 812 ATASGPLCDEPMWGLAFTIESHLAPAEDVETDKPENFGIFTGQVMTAVKDACRAAVLQTN 871
Query: 544 PRXMEPVYLCEI 509
PR +E +Y CE+
Sbjct: 872 PRIVEAMYFCEL 883
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 40.3 bits (90), Expect = 0.002
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = -2
Query: 543 PVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFT 364
PV+ + V+ VP G + G +N+R+G + Q ++ A +P+N FG++
Sbjct: 655 PVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYS 712
Query: 363 ADLRSNTGGQ 334
LRS T G+
Sbjct: 713 TSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 40.3 bits (90), Expect = 0.002
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = -2
Query: 543 PVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFT 364
PV+ + V+ VP G + G +N+R+G + Q ++ A +P+N FG++
Sbjct: 655 PVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYS 712
Query: 363 ADLRSNTGGQ 334
LRS T G+
Sbjct: 713 TSLRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 35.1 bits (77), Expect = 0.061
Identities = 20/62 (32%), Positives = 32/62 (51%)
Frame = -2
Query: 516 VKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 337
V+ PE +G + G LN RRG + G + +V + +P+ E F + + LR T G
Sbjct: 694 VEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQYVSTLRGMTKG 752
Query: 336 QA 331
+A
Sbjct: 753 RA 754
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 32.7 bits (71), Expect = 0.32
Identities = 18/72 (25%), Positives = 30/72 (41%)
Frame = -2
Query: 525 YIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSN 346
Y VPE +G + +L +RRG +F+ V ++ +P G + +
Sbjct: 478 YEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTA 537
Query: 345 TGGQAFPQCVFD 310
+ G A VFD
Sbjct: 538 SRGTAILNTVFD 549
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 32.7 bits (71), Expect = 0.32
Identities = 18/72 (25%), Positives = 30/72 (41%)
Frame = -2
Query: 525 YIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSN 346
Y VPE +G + +L +RRG +F+ V ++ +P G + +
Sbjct: 477 YEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTA 536
Query: 345 TGGQAFPQCVFD 310
+ G A VFD
Sbjct: 537 SRGTAILNTVFD 548
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 29.1 bits (62), Expect = 4.0
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +3
Query: 246 PVQRCRACSEVRTGLQG 296
PV RCR C+EV G QG
Sbjct: 62 PVHRCRLCAEVHIGKQG 78
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,263,045
Number of Sequences: 28952
Number of extensions: 331798
Number of successful extensions: 788
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 757
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 783
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2019160800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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