Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_J21
(807 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 225 4e-60
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 222 4e-59
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 82 8e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 7e-08
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.8
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.5
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.2
SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|c... 26 7.2
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 225 bits (551), Expect = 4e-60
Identities = 100/128 (78%), Positives = 115/128 (89%), Gaps = 1/128 (0%)
Frame = -3
Query: 511 WLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKV 335
++A C RGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV
Sbjct: 316 YMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKV 375
Query: 334 QRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKD 155
RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+D
Sbjct: 376 NRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERD 435
Query: 154 YEEVGMDS 131
YEEVG DS
Sbjct: 436 YEEVGQDS 443
Score = 132 bits (320), Expect = 4e-32
Identities = 57/73 (78%), Positives = 64/73 (87%)
Frame = -2
Query: 710 ALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCD 531
+LNVDL EFQTNLVPYPRIHFPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCD
Sbjct: 251 SLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCD 310
Query: 530 PRHGKYMACCMLY 492
PR G+YMA C+LY
Sbjct: 311 PRTGRYMATCLLY 323
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 222 bits (543), Expect = 4e-59
Identities = 100/131 (76%), Positives = 114/131 (87%), Gaps = 1/131 (0%)
Frame = -3
Query: 520 ASTWLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 344
A ++A C RGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++
Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368
Query: 343 AKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAL 164
AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAAL
Sbjct: 369 AKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 428
Query: 163 EKDYEEVGMDS 131
E+DYEEVG DS
Sbjct: 429 ERDYEEVGQDS 439
Score = 134 bits (323), Expect = 2e-32
Identities = 58/73 (79%), Positives = 64/73 (87%)
Frame = -2
Query: 710 ALNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCD 531
+LNVDL EFQTNLVPYPRIHFPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCD
Sbjct: 247 SLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCD 306
Query: 530 PRHGKYMACCMLY 492
PR G+YMA C+LY
Sbjct: 307 PRAGRYMATCLLY 319
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 82.2 bits (194), Expect = 8e-17
Identities = 44/122 (36%), Positives = 69/122 (56%), Gaps = 3/122 (2%)
Frame = -3
Query: 490 RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLS 311
RG V K+V+ I +++TK + FV+W P + PP DL + + +
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATFIG 369
Query: 310 NTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVG 140
N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA DL + + Y+E G
Sbjct: 370 NSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAG 429
Query: 139 MD 134
+D
Sbjct: 430 ID 431
Score = 69.7 bits (163), Expect = 4e-13
Identities = 29/72 (40%), Positives = 45/72 (62%)
Frame = -2
Query: 707 LNVDLTEFQTNLVPYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDP 528
LN DL + N+VP+PR+HF +V +AP+ + + + +SV E+T F+ N MV DP
Sbjct: 246 LNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADP 305
Query: 527 RHGKYMACCMLY 492
RHG+Y+ L+
Sbjct: 306 RHGRYLTVAALF 317
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.4 bits (120), Expect = 7e-08
Identities = 34/140 (24%), Positives = 69/140 (49%), Gaps = 4/140 (2%)
Frame = -3
Query: 556 PPTRW*NATPVMASTWLAVC-CTRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGIN 380
P + + P S ++++ +G+ P DV+ ++ I+ +R F+ W P +V ++
Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361
Query: 379 YQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE- 203
+ P + ++ + ML+N T+IA + R ++D + + AF+ Y E + E
Sbjct: 362 KKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFED 416
Query: 202 --GEFSEAREDLAALEKDYE 149
EF +R+ +A L +YE
Sbjct: 417 DLNEFDSSRDVVADLINEYE 436
Score = 37.5 bits (83), Expect = 0.002
Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = -2
Query: 707 LNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---AEKAYHEQLSVAEITNACFEPANQMVK 537
+N DL +L+P PR HF L +Y P + E + +V ++ P NQMV
Sbjct: 249 MNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVS 308
Query: 536 CDP 528
+P
Sbjct: 309 VNP 311
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.4
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -1
Query: 285 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 112
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 111 EPKSTK 94
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.9 bits (59), Expect = 1.8
Identities = 17/49 (34%), Positives = 26/49 (53%)
Frame = -3
Query: 691 PSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 545
P+++ P S + T +SS+P P N+ PSP S + AS+ P R
Sbjct: 1232 PTAKAPPVPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 26.2 bits (55), Expect = 5.5
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 628 QSSLPRRPTMNSFPSPRSQTHASSPPTR 545
+ SLPRRP+ +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765
>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 751
Score = 25.8 bits (54), Expect = 7.2
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = -3
Query: 694 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 554
SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664
>SPAC890.02c |alp7|mia1|TACC homolog |Schizosaccharomyces pombe|chr
1|||Manual
Length = 474
Score = 25.8 bits (54), Expect = 7.2
Identities = 16/51 (31%), Positives = 31/51 (60%)
Frame = -3
Query: 667 LTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTRW*NATPVMAS 515
LTP + S +S + +S +R ++N+ PS + +H+SS T + PV+++
Sbjct: 130 LTPKNPSLFSSSNAAS--QRGSLNTAPSNFAYSHSSSLQTSASSRPPVLSN 178
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,122,752
Number of Sequences: 5004
Number of extensions: 66771
Number of successful extensions: 220
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 392429240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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