Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_H23
(806 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 118 6e-27
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 92 3e-19
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 92 3e-19
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 90 2e-18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 72 4e-13
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 40 0.001
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 40 0.001
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 36 0.042
At5g13650.2 68418.m01585 elongation factor family protein contai... 34 0.097
At5g13650.1 68418.m01584 elongation factor family protein contai... 34 0.097
At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634... 31 1.2
At3g63180.1 68416.m07097 expressed protein 28 8.4
At1g09410.1 68414.m01052 pentatricopeptide (PPR) repeat-containi... 28 8.4
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 118 bits (283), Expect = 6e-27
Identities = 53/94 (56%), Positives = 67/94 (71%)
Frame = -2
Query: 541 PVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFT 362
P L V+ PE A+GGIY VLN++RGHVFEE Q GTP++ +KAYLPV ESFGF+
Sbjct: 723 PRLLEPVYMVEIQAPEGALGGIYSVLNQKRGHVFEEMQRPGTPLYNIKAYLPVVESFGFS 782
Query: 361 ADLRSNTGGQAFPQCVFDHWQVLPWRPVRTSEQA 260
+ LR+ T GQAFPQCVFDHW+++ P+ QA
Sbjct: 783 SQLRAATSGQAFPQCVFDHWEMMSSDPLEPGTQA 816
Score = 87.8 bits (208), Expect = 7e-18
Identities = 37/61 (60%), Positives = 48/61 (78%)
Frame = -1
Query: 665 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCP*SSC 486
+RG+ F + DV LH+DAIHRGGGQ+IPT RR +YA +TA+PRL+EPVY+ EIQ P +
Sbjct: 682 MRGICFEVCDVVLHSDAIHRGGGQVIPTARRVIYASQITAKPRLLEPVYMVEIQAPEGAL 741
Query: 485 G 483
G
Sbjct: 742 G 742
Score = 30.3 bits (65), Expect = 1.6
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -3
Query: 285 DPCEPQSKPYNVVQETRKRKGLKEGL 208
DP EP ++ +V + RKRKGLKE +
Sbjct: 808 DPLEPGTQASVLVADIRKRKGLKEAM 833
>At1g06220.2 68414.m00656 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 92.3 bits (219), Expect = 3e-19
Identities = 45/91 (49%), Positives = 57/91 (62%)
Frame = -2
Query: 550 LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 371
+ +P L +V+ P V IY VL+RRRGHV + GTP +IVKA+LPV ESF
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 370 GFTADLRSNTGGQAFPQCVFDHWQVLPWRPV 278
GF DLR +T GQAF VFDHW ++P P+
Sbjct: 896 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPL 926
Score = 68.5 bits (160), Expect = 5e-12
Identities = 30/56 (53%), Positives = 37/56 (66%)
Frame = -1
Query: 665 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCP 498
+R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P
Sbjct: 798 IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTP 853
>At1g06220.1 68414.m00655 elongation factor Tu family protein similar
to Cryptosporidium parvum elongation factor-2 GB:U21667
GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 92.3 bits (219), Expect = 3e-19
Identities = 45/91 (49%), Positives = 57/91 (62%)
Frame = -2
Query: 550 LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 371
+ +P L +V+ P V IY VL+RRRGHV + GTP +IVKA+LPV ESF
Sbjct: 836 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESF 895
Query: 370 GFTADLRSNTGGQAFPQCVFDHWQVLPWRPV 278
GF DLR +T GQAF VFDHW ++P P+
Sbjct: 896 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPL 926
Score = 68.5 bits (160), Expect = 5e-12
Identities = 30/56 (53%), Positives = 37/56 (66%)
Frame = -1
Query: 665 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCP 498
+R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P
Sbjct: 798 IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTP 853
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 89.8 bits (213), Expect = 2e-18
Identities = 44/91 (48%), Positives = 57/91 (62%)
Frame = -2
Query: 550 LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 371
+ +P L +V+ P V IY VL+RRRG+V + GTP +IVKA+LPV ESF
Sbjct: 822 MATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGYVTSDVPQPGTPAYIVKAFLPVIESF 881
Query: 370 GFTADLRSNTGGQAFPQCVFDHWQVLPWRPV 278
GF DLR +T GQAF VFDHW ++P P+
Sbjct: 882 GFETDLRYHTQGQAFCLSVFDHWAIVPGDPL 912
Score = 68.5 bits (160), Expect = 5e-12
Identities = 30/56 (53%), Positives = 37/56 (66%)
Frame = -1
Query: 665 LRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCP 498
+R V+F I D + + +HRG GQ+IPT RR Y+ L A PRLMEPVY EIQ P
Sbjct: 784 IRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTP 839
>At3g22980.1 68416.m02898 elongation factor Tu family protein similar
to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 72.1 bits (169), Expect = 4e-13
Identities = 35/100 (35%), Positives = 53/100 (53%)
Frame = -2
Query: 580 LEDACTHVC*LLSPVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIV 401
++DAC +P + F + + +G +Y VL+RRR + +E G+ +F V
Sbjct: 859 VKDACRAAVLQTNPRIVEAMYFCELNTAPEYLGPMYAVLSRRRARILKEEMQEGSSLFTV 918
Query: 400 KAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPWRP 281
AY+PV+ESFGF +LR T G A V HW++L P
Sbjct: 919 HAYVPVSESFGFADELRKGTSGGASALMVLSHWEMLEEDP 958
Score = 29.1 bits (62), Expect = 3.6
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = -1
Query: 599 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEI 507
GQ++ + A +L PR++E +Y CE+
Sbjct: 853 GQVMTAVKDACRAAVLQTNPRIVEAMYFCEL 883
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 40.3 bits (90), Expect = 0.001
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = -2
Query: 541 PVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFT 362
PV+ + V+ VP G + G +N+R+G + Q ++ A +P+N FG++
Sbjct: 655 PVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYS 712
Query: 361 ADLRSNTGGQ 332
LRS T G+
Sbjct: 713 TSLRSMTQGK 722
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 40.3 bits (90), Expect = 0.001
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = -2
Query: 541 PVLWSLYIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFT 362
PV+ + V+ VP G + G +N+R+G + Q ++ A +P+N FG++
Sbjct: 655 PVILEPVMLVELKVPTEFQGTVAGDINKRKGIIVGNDQEGDDS--VITANVPLNNMFGYS 712
Query: 361 ADLRSNTGGQ 332
LRS T G+
Sbjct: 713 TSLRSMTQGK 722
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 35.5 bits (78), Expect = 0.042
Identities = 22/75 (29%), Positives = 36/75 (48%)
Frame = -2
Query: 514 VKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSNTGG 335
V+ PE +G + G LN RRG + G + +V + +P+ E F + + LR T G
Sbjct: 694 VEVVTPEEHLGDVIGDLNSRRGQINSFGDKPG-GLKVVDSLVPLAEMFQYVSTLRGMTKG 752
Query: 334 QAFPQCVFDHWQVLP 290
+A + V+P
Sbjct: 753 RASYTMQLAKFDVVP 767
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 34.3 bits (75), Expect = 0.097
Identities = 20/79 (25%), Positives = 33/79 (41%)
Frame = -2
Query: 523 YIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSN 344
Y VPE +G + +L +RRG +F+ V ++ +P G + +
Sbjct: 478 YEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTA 537
Query: 343 TGGQAFPQCVFDHWQVLPW 287
+ G A VFD + PW
Sbjct: 538 SRGTAILNTVFDSYG--PW 554
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 34.3 bits (75), Expect = 0.097
Identities = 20/79 (25%), Positives = 33/79 (41%)
Frame = -2
Query: 523 YIFVKFSVPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRSN 344
Y VPE +G + +L +RRG +F+ V ++ +P G + +
Sbjct: 477 YEIATVEVPEAHMGPVVELLGKRRGQMFDMQGVGSEGTTFLRYKIPTRGLLGLRNAILTA 536
Query: 343 TGGQAFPQCVFDHWQVLPW 287
+ G A VFD + PW
Sbjct: 537 SRGTAILNTVFDSYG--PW 553
>At5g61930.1 68418.m07774 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 340
Score = 30.7 bits (66), Expect = 1.2
Identities = 14/23 (60%), Positives = 15/23 (65%), Gaps = 3/23 (13%)
Frame = +3
Query: 243 PVQRCRACSEVRTGLQG---RTC 302
PV RCR C+EV G QG RTC
Sbjct: 62 PVHRCRLCAEVHIGKQGHEIRTC 84
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 27.9 bits (59), Expect = 8.4
Identities = 15/55 (27%), Positives = 25/55 (45%), Gaps = 5/55 (9%)
Frame = -3
Query: 396 PTYLSMSRSVLLPICVPTPADRPSRSAYSTIGR-----SXPGDPCEPQSKPYNVV 247
P ++ + L P+ VP+ + RP++S + +G S P S PY V
Sbjct: 539 PAFIYPANHHLQPVMVPSKSSRPTKSPHLAVGLASANFSHPSSSASEVSSPYLTV 593
>At1g09410.1 68414.m01052 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 705
Score = 27.9 bits (59), Expect = 8.4
Identities = 15/48 (31%), Positives = 24/48 (50%)
Frame = +3
Query: 453 RRLFSTP*IPPTATSGTLNFTKIYRLHKTGLSS*QTCVQASSSCWNDL 596
RR +ST PPTA + ++I ++H+ +C S S WN +
Sbjct: 8 RRTYSTTIPPPTANVRITHLSRIGKIHE-ARKLFDSCDSKSISSWNSM 54
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,988,379
Number of Sequences: 28952
Number of extensions: 333905
Number of successful extensions: 767
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 735
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 765
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1833827200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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