Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_E22
(818 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 144 2e-35
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 143 2e-35
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 65 1e-11
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 35 0.012
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 33 0.064
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.4
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 2.4
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.6
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.6
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 9.8
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 144 bits (348), Expect = 2e-35
Identities = 66/80 (82%), Positives = 71/80 (88%)
Frame = -3
Query: 381 PPWCPEATWPKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFS 202
P P + KV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFS
Sbjct: 364 PQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFS 423
Query: 201 EAREDLAALEKDYEEVGMDS 142
EAREDLAALE+DYEEVG DS
Sbjct: 424 EAREDLAALERDYEEVGQDS 443
Score = 103 bits (248), Expect = 2e-23
Identities = 44/60 (73%), Positives = 50/60 (83%)
Frame = -1
Query: 683 VXXPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 504
V PRIHFPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 264 VPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLY 323
Score = 87.4 bits (207), Expect = 2e-18
Identities = 36/59 (61%), Positives = 48/59 (81%), Gaps = 1/59 (1%)
Frame = -2
Query: 523 WLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAQ 350
++A C RGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +A+
Sbjct: 316 YMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAK 374
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 143 bits (347), Expect = 2e-35
Identities = 68/92 (73%), Positives = 74/92 (80%), Gaps = 1/92 (1%)
Frame = -3
Query: 414 PVSRSVSTTSHPPWCPEATW-PKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHW 238
P + PP E + KV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHW
Sbjct: 348 PTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHW 407
Query: 237 YVGEGMEEGEFSEAREDLAALEKDYEEVGMDS 142
YVGEGMEEGEFSEAREDLAALE+DYEEVG DS
Sbjct: 408 YVGEGMEEGEFSEAREDLAALERDYEEVGQDS 439
Score = 105 bits (251), Expect = 1e-23
Identities = 45/60 (75%), Positives = 50/60 (83%)
Frame = -1
Query: 683 VXXPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 504
V PRIHFPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 260 VPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLY 319
Score = 84.2 bits (199), Expect = 2e-17
Identities = 36/62 (58%), Positives = 47/62 (75%), Gaps = 1/62 (1%)
Frame = -2
Query: 532 ASTWLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 356
A ++A C RGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++
Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368
Query: 355 AQ 350
A+
Sbjct: 369 AK 370
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 64.9 bits (151), Expect = 1e-11
Identities = 30/74 (40%), Positives = 48/74 (64%), Gaps = 4/74 (5%)
Frame = -3
Query: 354 PK-VQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---ED 187
PK ++ + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA D
Sbjct: 358 PKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMND 417
Query: 186 LAALEKDYEEVGMD 145
L + + Y+E G+D
Sbjct: 418 LVSEYQQYQEAGID 431
Score = 54.8 bits (126), Expect = 1e-08
Identities = 23/60 (38%), Positives = 36/60 (60%)
Frame = -1
Query: 683 VXXPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 504
V PR+HF +V +AP+ + + + +SV E+T F+ N MV DPRHG+Y+ L+
Sbjct: 258 VPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALF 317
Score = 32.3 bits (70), Expect = 0.085
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 502 RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 380
RG V K+V+ I +++TK + FV+W P + PP
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPP 358
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 35.1 bits (77), Expect = 0.012
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -3
Query: 510 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 367
A PVV P + RP++P P LS V PV+ V + PP P
Sbjct: 549 AAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 29.5 bits (63), Expect = 0.60
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -3
Query: 510 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 367
A PVV P + RP++P P S PV V + PP P
Sbjct: 639 AAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
Score = 27.1 bits (57), Expect = 3.2
Identities = 18/49 (36%), Positives = 21/49 (42%)
Frame = -3
Query: 507 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 361
VP V S P+ P +P PSV + V P SV P PEA
Sbjct: 643 VPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVPVVPEA 691
Score = 26.6 bits (56), Expect = 4.2
Identities = 16/47 (34%), Positives = 20/47 (42%)
Frame = -3
Query: 504 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 364
P V S P+ P +P PSV + V P + SV P PE
Sbjct: 539 PEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPE 585
Score = 26.2 bits (55), Expect = 5.6
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -3
Query: 507 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 364
VP S P+ P +P PSV + V P + SV P PE
Sbjct: 628 VPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 675
Score = 25.4 bits (53), Expect = 9.8
Identities = 16/48 (33%), Positives = 19/48 (39%)
Frame = -3
Query: 507 VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPE 364
VP S P+ P P PSV + V P + SV P PE
Sbjct: 598 VPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPE 645
Score = 25.4 bits (53), Expect = 9.8
Identities = 17/48 (35%), Positives = 20/48 (41%)
Frame = -3
Query: 504 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 361
P V S P+ P +P PSV + V P SV P PEA
Sbjct: 614 PEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEA 661
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 32.7 bits (71), Expect = 0.064
Identities = 14/66 (21%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = -2
Query: 568 PPTRW*NATPVMASTWLAVC-CTRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGIN 392
P + + P S ++++ +G+ P DV+ ++ I+ +R F+ W P +V ++
Sbjct: 302 PKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALS 361
Query: 391 YQPPTV 374
+ P +
Sbjct: 362 KKSPYI 367
Score = 27.9 bits (59), Expect = 1.8
Identities = 15/51 (29%), Positives = 23/51 (45%), Gaps = 3/51 (5%)
Frame = -1
Query: 683 VXXPRIHFPLVTYAPVIS---AEKAYHEQLSVAEITNACFEPANQMVKCDP 540
+ PR HF L +Y P + E + +V ++ P NQMV +P
Sbjct: 261 IPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNP 311
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.4
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -1
Query: 296 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 123
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 122 EPKSTK 105
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 2.4
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = -2
Query: 673 PVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 557
P S + T +SS+P P N+ PSP S + AS+ P R
Sbjct: 1242 PSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 27.5 bits (58), Expect = 2.4
Identities = 23/70 (32%), Positives = 31/70 (44%), Gaps = 1/70 (1%)
Frame = -3
Query: 504 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATWPK-VQRAVCM 328
PVV + P T+P S P+ S + PVS + T + PP P Q A +
Sbjct: 988 PVVQNKPAA-TKPV--SMPAAKSKPAPMANPVSTAQQTQNRPPAPAMQARPNTTQAAAPV 1044
Query: 327 LSNTTAIAEA 298
S TT I +A
Sbjct: 1045 TSTTTTIKQA 1054
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 640 QSSLPRRPTMNSFPSPRSQTHASSPPTR 557
+ SLPRRP+ +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.6
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -3
Query: 465 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 379
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 9.8
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -3
Query: 468 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 373
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,914,980
Number of Sequences: 5004
Number of extensions: 61407
Number of successful extensions: 243
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 215
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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