Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_T7_D06
(843 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 225 5e-60
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 222 4e-59
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 82 8e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 8e-08
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 35 0.013
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.4
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 27 4.4
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.8
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 5.8
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.7
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 225 bits (551), Expect = 5e-60
Identities = 100/128 (78%), Positives = 115/128 (89%), Gaps = 1/128 (0%)
Frame = -2
Query: 521 WLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKV 345
++A C RGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV
Sbjct: 316 YMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKV 375
Query: 344 QRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKD 165
RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+D
Sbjct: 376 NRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERD 435
Query: 164 YEEVGMDS 141
YEEVG DS
Sbjct: 436 YEEVGQDS 443
Score = 86.2 bits (204), Expect = 5e-18
Identities = 37/48 (77%), Positives = 41/48 (85%)
Frame = -3
Query: 709 DLTEFQTNLVPXPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEP 566
DL EFQTNLVP PRIHFPLVTY+P++SA KA+HE SV EITN CFEP
Sbjct: 255 DLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEP 302
Score = 44.0 bits (99), Expect = 3e-05
Identities = 17/22 (77%), Positives = 19/22 (86%)
Frame = -1
Query: 567 PPNQMVKCDPRHGKYMACCMLY 502
P NQMVKCDPR G+YMA C+LY
Sbjct: 302 PYNQMVKCDPRTGRYMATCLLY 323
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 222 bits (543), Expect = 4e-59
Identities = 100/131 (76%), Positives = 114/131 (87%), Gaps = 1/131 (0%)
Frame = -2
Query: 530 ASTWLAVCCT-RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDL 354
A ++A C RGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++
Sbjct: 309 AGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEI 368
Query: 353 AKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAAL 174
AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAAL
Sbjct: 369 AKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAAL 428
Query: 173 EKDYEEVGMDS 141
E+DYEEVG DS
Sbjct: 429 ERDYEEVGQDS 439
Score = 87.4 bits (207), Expect = 2e-18
Identities = 38/48 (79%), Positives = 41/48 (85%)
Frame = -3
Query: 709 DLTEFQTNLVPXPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEP 566
DL EFQTNLVP PRIHFPLVTYAP++SA KA+HE SV EITN CFEP
Sbjct: 251 DLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEP 298
Score = 44.0 bits (99), Expect = 3e-05
Identities = 17/22 (77%), Positives = 19/22 (86%)
Frame = -1
Query: 567 PPNQMVKCDPRHGKYMACCMLY 502
P NQMVKCDPR G+YMA C+LY
Sbjct: 298 PYNQMVKCDPRAGRYMATCLLY 319
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 82.2 bits (194), Expect = 8e-17
Identities = 44/122 (36%), Positives = 69/122 (56%), Gaps = 3/122 (2%)
Frame = -2
Query: 500 RGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLS 321
RG V K+V+ I +++TK + FV+W P + PP DL + + +
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK-----DL---KMSATFIG 369
Query: 320 NTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVG 150
N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA DL + + Y+E G
Sbjct: 370 NSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAG 429
Query: 149 MD 144
+D
Sbjct: 430 ID 431
Score = 41.1 bits (92), Expect = 2e-04
Identities = 17/47 (36%), Positives = 29/47 (61%)
Frame = -3
Query: 709 DLTEFQTNLVPXPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFE 569
DL + N+VP PR+HF +V +AP+ + + + +SV E+T F+
Sbjct: 249 DLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFD 295
Score = 29.1 bits (62), Expect = 0.82
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 561 NQMVKCDPRHGKYMACCMLY 502
N MV DPRHG+Y+ L+
Sbjct: 298 NMMVAADPRHGRYLTVAALF 317
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.4 bits (120), Expect = 8e-08
Identities = 36/148 (24%), Positives = 72/148 (48%), Gaps = 4/148 (2%)
Frame = -2
Query: 590 DHKRMLRARQTRW*NATPVMASTWLAVC-CTRGDVVPKDVNAAIATIKTKRTIQFVDWCP 414
D R L + + + P S ++++ +G+ P DV+ ++ I+ +R F+ W P
Sbjct: 294 DVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGP 353
Query: 413 TGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWY 234
+V ++ + P + ++ + ML+N T+IA + R ++D + + AF+ Y
Sbjct: 354 ASIQVALSKKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFLEQY 408
Query: 233 VGEGMEE---GEFSEAREDLAALEKDYE 159
E + E EF +R+ +A L +YE
Sbjct: 409 KKEAIFEDDLNEFDSSRDVVADLINEYE 436
Score = 29.1 bits (62), Expect = 0.82
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -3
Query: 709 DLTEFQTNLVPXPRIHFPLVTYAP 638
DL +L+P PR HF L +Y P
Sbjct: 252 DLVSIIASLIPSPRCHFLLTSYTP 275
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 35.1 bits (77), Expect = 0.013
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = -3
Query: 508 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 365
A PVV P + RP++P P LS V PV+ V + PP P
Sbjct: 549 AAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 29.5 bits (63), Expect = 0.62
Identities = 16/48 (33%), Positives = 20/48 (41%)
Frame = -3
Query: 508 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 365
A PVV P + RP++P P S PV V + PP P
Sbjct: 639 AAPVVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
Score = 28.3 bits (60), Expect = 1.4
Identities = 31/110 (28%), Positives = 41/110 (37%), Gaps = 2/110 (1%)
Frame = -3
Query: 682 VPXPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPAKPDGEM--RPPSWQVHGLLY 509
VP P + P+V AP + V + P P+ +PP+ V
Sbjct: 589 VPQPPVA-PVVPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPV----- 642
Query: 508 AVPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 359
VP V S P+ P +P PSV + V P SV P PEA
Sbjct: 643 -VPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVPVVPEA 691
Score = 27.9 bits (59), Expect = 1.9
Identities = 22/71 (30%), Positives = 28/71 (39%), Gaps = 2/71 (2%)
Frame = -3
Query: 568 PAKPDGEMRPPSWQVHGLLYA--VPVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSV 395
P P + P + VH A P V S P+ P +P PSV + V P + SV
Sbjct: 515 PQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSV 574
Query: 394 STTSHPPWCPE 362
P PE
Sbjct: 575 PQPPVAPVAPE 585
Score = 27.5 bits (58), Expect = 2.5
Identities = 25/100 (25%), Positives = 38/100 (38%), Gaps = 2/100 (2%)
Frame = -3
Query: 658 PLVTYAPVISAEKAYHEQLSVAEITNACFEPAKPDGEMRP--PSWQVHGLLYAVPVVTSY 485
P APV + ++ +V + A P P + P PS + VP S
Sbjct: 605 PQPPVAPVAPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQRPAVPVVPEAPSV 664
Query: 484 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 365
P+ P +P PSV + V P + ++ PP P
Sbjct: 665 PQPPAAPVVPEVPSVPQPPAVPVVPEAGQLNEPVVPPLPP 704
Score = 26.6 bits (56), Expect = 4.4
Identities = 29/107 (27%), Positives = 40/107 (37%), Gaps = 2/107 (1%)
Frame = -3
Query: 673 PRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPAKPDGEMRPPSWQVHGLLYA--VP 500
P +H P APV + ++ + + A P +P + P + V A P
Sbjct: 527 PSVHQPPA--APVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAP 584
Query: 499 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEA 359
V S P+ P +P PSV V P SV P PEA
Sbjct: 585 EVPSVPQPPVAPVVPEAPSVPQPPVAPVAPEVPSVPQRPAVPVVPEA 631
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.4
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -3
Query: 295 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 122
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 121 EPKSTK 104
EP+ TK
Sbjct: 208 EPRFTK 213
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 26.6 bits (56), Expect = 4.4
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -1
Query: 609 NSFPSPRSQTHASSPPNQMVKCDPRH 532
N+ PS + ++ S+ PNQ +K P+H
Sbjct: 234 NAPPSIPANSNNSASPNQRIKASPKH 259
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.8
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -3
Query: 463 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 377
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.2 bits (55), Expect = 5.8
Identities = 26/101 (25%), Positives = 40/101 (39%)
Frame = -3
Query: 502 PVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCPEATWPRFNVXXXXX 323
PVV + P T+P S P+ S + PVS + T + PP A R N
Sbjct: 988 PVVQNKPAA-TKPV--SMPAAKSKPAPMANPVSTAQQTQNRPP--APAMQARPNTTQAAA 1042
Query: 322 XXXXXXPKLGLALTTSSTSCTPSVLSCTGTSVRVWRRESSP 200
+ A T S++ PS ++ +S + S+P
Sbjct: 1043 PVTSTTTTIKQATTVSASKPAPSTVTSAASSPSNISKPSAP 1083
>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 751
Score = 25.8 bits (54), Expect = 7.7
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = -1
Query: 705 SPSSRLTWCLTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSP 565
SPS LT L S++ S + + + R+ T +S SP+ Q+ S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,086,551
Number of Sequences: 5004
Number of extensions: 66765
Number of successful extensions: 260
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 229
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 255
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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