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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_T7_A16
         (819 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g09140.1 68417.m01509 DNA mismatch repair protein MLH1 (MLH1)...    83   6e-27
At2g41140.1 68415.m05081 calcium-dependent protein kinase, putat...    30   2.1  
At5g65590.1 68418.m08252 Dof-type zinc finger domain-containing ...    29   2.8  
At5g40320.1 68418.m04892 DC1 domain-containing protein contains ...    29   4.9  
At3g21530.1 68416.m02716 endonuclease/exonuclease/phosphatase fa...    29   4.9  
At3g07050.1 68416.m00837 GTP-binding family protein contains Pfa...    28   6.5  
At3g24800.1 68416.m03112 PRT1 protein (PRT1) E3, N-end rule ubiq...    28   8.6  
At2g44130.1 68415.m05489 kelch repeat-containing F-box family pr...    28   8.6  

>At4g09140.1 68417.m01509 DNA mismatch repair protein MLH1 (MLH1)
            identical to MLH1 protein [Arabidopsis thaliana]
            GI:3893081
          Length = 737

 Score = 83.0 bits (196), Expect(2) = 6e-27
 Identities = 52/183 (28%), Positives = 84/183 (45%), Gaps = 3/183 (1%)
 Frame = -2

Query: 734  NXXFIARIDCHKSLIQHXTKLYLCDXXXXXXXXXXXXXLYDFQNFGFIKLSNPLPLEELF 555
            N  ++   D   +L+Q+ T LYL +             L  F +F  I+LS+P PL EL 
Sbjct: 478  NCTYVGMADDVFALVQYNTHLYLANVVNLSKELMYQQTLRRFAHFNAIQLSDPAPLSELI 537

Query: 554  VLGLNGSTQNSEQDSELGDTGEMAQHMTQLLLSKRAMLYEYFSLEVNGEGEXXXXXXXLD 375
            +L L     +   D++      +A+  T+LL  K  ML EYFS+ ++           LD
Sbjct: 538  LLALKEEDLDPGNDTKDDLKERIAEMNTELLKEKAEMLEEYFSVHIDSSANLSRLPVILD 597

Query: 374  GHAPFVGALPTYLVRLVTEVNWDSEKECFETFCRQTAKFYAQPN---PDPNVESVSSEHR 204
             + P +  +P +L+ L  +V W+ EK CF+        FYA      P+P+ + +    +
Sbjct: 598  QYTPDMDRVPEFLLCLGNDVEWEDEKSCFQGVSAAIGNFYAMHPPLLPNPSGDGIQFYSK 657

Query: 203  RQE 195
            R E
Sbjct: 658  RGE 660



 Score = 56.0 bits (129), Expect(2) = 6e-27
 Identities = 21/40 (52%), Positives = 31/40 (77%)
 Frame = -2

Query: 197 EHVIFPAIRKYFLPPSSFISNGAILQIASLNDLYKVFERC 78
           +HV+FP++R +  PP+S  SNG  +++ASL  LYK+FERC
Sbjct: 698 QHVLFPSMRLFLKPPASMASNGTFVKVASLEKLYKIFERC 737


>At2g41140.1 68415.m05081 calcium-dependent protein kinase, putative
           / CDPK, putative similar to calcium/calmodulin-dependent
           protein kinase CaMK3 [Nicotiana tabacum]
           gi|16904226|gb|AAL30820
          Length = 576

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 16/40 (40%), Positives = 25/40 (62%)
 Frame = +1

Query: 349 KAPTNGA*PSKSNGRANNSPSPFTSREKYS*SIARLLNNS 468
           +APTN   P+KS+G    SPSP  S  K S S++  ++++
Sbjct: 24  EAPTNSQPPAKSSGFPFYSPSPVPSLFKSSPSVSSSVSST 63


>At5g65590.1 68418.m08252 Dof-type zinc finger domain-containing
           protein 
          Length = 316

 Score = 29.5 bits (63), Expect = 2.8
 Identities = 20/60 (33%), Positives = 28/60 (46%)
 Frame = -2

Query: 278 CRQTAKFYAQPNPDPNVESVSSEHRRQEHVIFPAIRKYFLPPSSFISNGAILQIASLNDL 99
           CR+T K   +PN   N    SS  +R    I     K+F PP++     A L +  +NDL
Sbjct: 90  CRKTKKSI-KPNSSMNTLPSSSSSQRFFSSIMEDSSKFFPPPTTMDFQLAGLSLNKMNDL 148


>At5g40320.1 68418.m04892 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 594

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +2

Query: 365 ERDHPKVMVGPIILLLHLLPEKSIRRALRVCSTIVGSYV 481
           E +HP     P++LL H LP+++     R+C   VG  V
Sbjct: 99  EINHPSHQRHPLMLLTHGLPQEAEDDKCRLCGEKVGKLV 137


>At3g21530.1 68416.m02716 endonuclease/exonuclease/phosphatase
           family protein contains Pfam profile PF03372:
           Endonuclease/Exonuclease/phosphatase family
          Length = 458

 Score = 28.7 bits (61), Expect = 4.9
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = -3

Query: 334 SVSLPKSIGILKRSALRLSADRRPSFMLNL 245
           +++ P   GILK+S L  SA R+P   +NL
Sbjct: 95  NITCPSPKGILKQSPLHSSAVRKPKVCINL 124


>At3g07050.1 68416.m00837 GTP-binding family protein contains Pfam
           domain, PF01926: GTPase of unknown function
          Length = 582

 Score = 28.3 bits (60), Expect = 6.5
 Identities = 13/43 (30%), Positives = 21/43 (48%)
 Frame = +2

Query: 422 PEKSIRRALRVCSTIVGSYVEPFLLYLRAQNPVRSFECSRSDQ 550
           P K +   L     +     E +L+YLR + P  +F+CS  +Q
Sbjct: 166 PNKHLVLLLNKIDLVPREAAEKWLMYLREEFPAVAFKCSTQEQ 208


>At3g24800.1 68416.m03112 PRT1 protein (PRT1) E3, N-end rule
           ubiquitin ligase, contains two RING finger domain;
           identical to PRT1 [Arabidopsis thaliana] GI:3319884
          Length = 410

 Score = 27.9 bits (59), Expect = 8.6
 Identities = 12/36 (33%), Positives = 19/36 (52%)
 Frame = -2

Query: 320 EVNWDSEKECFETFCRQTAKFYAQPNPDPNVESVSS 213
           E+ +D  K+C+ET  +   +F  Q  PD  +E   S
Sbjct: 332 EIGYDLCKDCYETPSKVPGRFNQQHTPDHRLELARS 367


>At2g44130.1 68415.m05489 kelch repeat-containing F-box family
           protein very low similarity to SP|Q9ER30 Kelch-related
           protein 1 (Sarcosin) {Rattus norvegicus}; contains Pfam
           profiles PF01344: Kelch motif, PF00646: F-box domain
          Length = 409

 Score = 27.9 bits (59), Expect = 8.6
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = -1

Query: 477 YDPTIVEQTRNALRILFSGSKWRR 406
           +DP  ++ TR+   + F+G KWRR
Sbjct: 158 WDPETLQPTRDVYVLEFAGRKWRR 181


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,709,810
Number of Sequences: 28952
Number of extensions: 317813
Number of successful extensions: 805
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 788
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 805
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1872844800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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