BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_T7_A16 (819 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g09140.1 68417.m01509 DNA mismatch repair protein MLH1 (MLH1)... 83 6e-27 At2g41140.1 68415.m05081 calcium-dependent protein kinase, putat... 30 2.1 At5g65590.1 68418.m08252 Dof-type zinc finger domain-containing ... 29 2.8 At5g40320.1 68418.m04892 DC1 domain-containing protein contains ... 29 4.9 At3g21530.1 68416.m02716 endonuclease/exonuclease/phosphatase fa... 29 4.9 At3g07050.1 68416.m00837 GTP-binding family protein contains Pfa... 28 6.5 At3g24800.1 68416.m03112 PRT1 protein (PRT1) E3, N-end rule ubiq... 28 8.6 At2g44130.1 68415.m05489 kelch repeat-containing F-box family pr... 28 8.6 >At4g09140.1 68417.m01509 DNA mismatch repair protein MLH1 (MLH1) identical to MLH1 protein [Arabidopsis thaliana] GI:3893081 Length = 737 Score = 83.0 bits (196), Expect(2) = 6e-27 Identities = 52/183 (28%), Positives = 84/183 (45%), Gaps = 3/183 (1%) Frame = -2 Query: 734 NXXFIARIDCHKSLIQHXTKLYLCDXXXXXXXXXXXXXLYDFQNFGFIKLSNPLPLEELF 555 N ++ D +L+Q+ T LYL + L F +F I+LS+P PL EL Sbjct: 478 NCTYVGMADDVFALVQYNTHLYLANVVNLSKELMYQQTLRRFAHFNAIQLSDPAPLSELI 537 Query: 554 VLGLNGSTQNSEQDSELGDTGEMAQHMTQLLLSKRAMLYEYFSLEVNGEGEXXXXXXXLD 375 +L L + D++ +A+ T+LL K ML EYFS+ ++ LD Sbjct: 538 LLALKEEDLDPGNDTKDDLKERIAEMNTELLKEKAEMLEEYFSVHIDSSANLSRLPVILD 597 Query: 374 GHAPFVGALPTYLVRLVTEVNWDSEKECFETFCRQTAKFYAQPN---PDPNVESVSSEHR 204 + P + +P +L+ L +V W+ EK CF+ FYA P+P+ + + + Sbjct: 598 QYTPDMDRVPEFLLCLGNDVEWEDEKSCFQGVSAAIGNFYAMHPPLLPNPSGDGIQFYSK 657 Query: 203 RQE 195 R E Sbjct: 658 RGE 660 Score = 56.0 bits (129), Expect(2) = 6e-27 Identities = 21/40 (52%), Positives = 31/40 (77%) Frame = -2 Query: 197 EHVIFPAIRKYFLPPSSFISNGAILQIASLNDLYKVFERC 78 +HV+FP++R + PP+S SNG +++ASL LYK+FERC Sbjct: 698 QHVLFPSMRLFLKPPASMASNGTFVKVASLEKLYKIFERC 737 >At2g41140.1 68415.m05081 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium/calmodulin-dependent protein kinase CaMK3 [Nicotiana tabacum] gi|16904226|gb|AAL30820 Length = 576 Score = 29.9 bits (64), Expect = 2.1 Identities = 16/40 (40%), Positives = 25/40 (62%) Frame = +1 Query: 349 KAPTNGA*PSKSNGRANNSPSPFTSREKYS*SIARLLNNS 468 +APTN P+KS+G SPSP S K S S++ ++++ Sbjct: 24 EAPTNSQPPAKSSGFPFYSPSPVPSLFKSSPSVSSSVSST 63 >At5g65590.1 68418.m08252 Dof-type zinc finger domain-containing protein Length = 316 Score = 29.5 bits (63), Expect = 2.8 Identities = 20/60 (33%), Positives = 28/60 (46%) Frame = -2 Query: 278 CRQTAKFYAQPNPDPNVESVSSEHRRQEHVIFPAIRKYFLPPSSFISNGAILQIASLNDL 99 CR+T K +PN N SS +R I K+F PP++ A L + +NDL Sbjct: 90 CRKTKKSI-KPNSSMNTLPSSSSSQRFFSSIMEDSSKFFPPPTTMDFQLAGLSLNKMNDL 148 >At5g40320.1 68418.m04892 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 594 Score = 28.7 bits (61), Expect = 4.9 Identities = 14/39 (35%), Positives = 21/39 (53%) Frame = +2 Query: 365 ERDHPKVMVGPIILLLHLLPEKSIRRALRVCSTIVGSYV 481 E +HP P++LL H LP+++ R+C VG V Sbjct: 99 EINHPSHQRHPLMLLTHGLPQEAEDDKCRLCGEKVGKLV 137 >At3g21530.1 68416.m02716 endonuclease/exonuclease/phosphatase family protein contains Pfam profile PF03372: Endonuclease/Exonuclease/phosphatase family Length = 458 Score = 28.7 bits (61), Expect = 4.9 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = -3 Query: 334 SVSLPKSIGILKRSALRLSADRRPSFMLNL 245 +++ P GILK+S L SA R+P +NL Sbjct: 95 NITCPSPKGILKQSPLHSSAVRKPKVCINL 124 >At3g07050.1 68416.m00837 GTP-binding family protein contains Pfam domain, PF01926: GTPase of unknown function Length = 582 Score = 28.3 bits (60), Expect = 6.5 Identities = 13/43 (30%), Positives = 21/43 (48%) Frame = +2 Query: 422 PEKSIRRALRVCSTIVGSYVEPFLLYLRAQNPVRSFECSRSDQ 550 P K + L + E +L+YLR + P +F+CS +Q Sbjct: 166 PNKHLVLLLNKIDLVPREAAEKWLMYLREEFPAVAFKCSTQEQ 208 >At3g24800.1 68416.m03112 PRT1 protein (PRT1) E3, N-end rule ubiquitin ligase, contains two RING finger domain; identical to PRT1 [Arabidopsis thaliana] GI:3319884 Length = 410 Score = 27.9 bits (59), Expect = 8.6 Identities = 12/36 (33%), Positives = 19/36 (52%) Frame = -2 Query: 320 EVNWDSEKECFETFCRQTAKFYAQPNPDPNVESVSS 213 E+ +D K+C+ET + +F Q PD +E S Sbjct: 332 EIGYDLCKDCYETPSKVPGRFNQQHTPDHRLELARS 367 >At2g44130.1 68415.m05489 kelch repeat-containing F-box family protein very low similarity to SP|Q9ER30 Kelch-related protein 1 (Sarcosin) {Rattus norvegicus}; contains Pfam profiles PF01344: Kelch motif, PF00646: F-box domain Length = 409 Score = 27.9 bits (59), Expect = 8.6 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = -1 Query: 477 YDPTIVEQTRNALRILFSGSKWRR 406 +DP ++ TR+ + F+G KWRR Sbjct: 158 WDPETLQPTRDVYVLEFAGRKWRR 181 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,709,810 Number of Sequences: 28952 Number of extensions: 317813 Number of successful extensions: 805 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 788 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 805 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1872844800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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