Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_O13
(825 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 183 1e-46
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 180 8e-46
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 179 2e-45
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 177 6e-45
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 115 4e-26
At1g54860.1 68414.m06263 expressed protein 28 6.5
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 183 bits (446), Expect = 1e-46
Identities = 87/184 (47%), Positives = 123/184 (66%)
Frame = +1
Query: 196 AFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIG 375
A G+ +G++IWRI+NF+P+ V Q+ GKF+ GDSYI+L+TT+ +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 376 RESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFN 555
++S+QDE+GA A++TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SGFN
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFN 130
Query: 556 HVVTNAGAQKRMFQVKGKRDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVGXKAKNVEX 735
HV Q R++ KGK VRV++V +++N DVF+LD + I+ F G K+ E
Sbjct: 131 HVKPEE-HQTRLYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQER 189
Query: 736 LKAI 747
KA+
Sbjct: 190 AKAL 193
Score = 49.6 bits (113), Expect = 2e-06
Identities = 45/216 (20%), Positives = 90/216 (41%), Gaps = 10/216 (4%)
Frame = +1
Query: 94 GSVWARTATKPQEISGPITSLSDKNARDKAKVHPAFANVGRTAGVQIWRIQNFEPIPVAQ 273
G V A + + G + S +++D+ K + + T +Q+WRI E I +
Sbjct: 358 GKVAALLQRQGVNVQG-LVKTSSSSSKDEPKPY-----IDGTGNLQVWRINCEEKILLEA 411
Query: 274 KDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAA 453
+ KFY GD Y IL+ + + + W G++S +++ +A L + +
Sbjct: 412 AEQSKFYSGDCY-ILQYSYPGEDREEHLVGTWFGKQSVEEDRASAISLANKMVESMKFVP 470
Query: 454 VQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVV--------TNAGAQKRMFQVKGK 609
Q R G E F Q+ + + +GG F + T +F+V+G
Sbjct: 471 AQARINEGKEPIQFFVIMQSFITF-KGGVSDAFKKYIAENDIPDTTYEAEGVALFRVQGS 529
Query: 610 --RDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVG 711
+++ Q++ A +N ++L + ++ + G
Sbjct: 530 GPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCG 565
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 180 bits (439), Expect = 8e-46
Identities = 88/185 (47%), Positives = 119/185 (64%)
Frame = +1
Query: 193 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 372
PAF G+ G +IWRI+NFE +PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 373 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 552
G++++QDE+G AA+ TV LD GG AVQHRE GHES FLSYF+ + LEGG SGF
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 553 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVGXKAKNVE 732
V + R++ KGKR +R++QV +S+N DVF+LD IY F G + E
Sbjct: 128 K-TVEEEVFETRLYTCKGKRAIRLKQVPFARSSLNHDDVFILDTEEKIYQFNGANSNIQE 186
Query: 733 XLKAI 747
KA+
Sbjct: 187 RAKAL 191
Score = 65.3 bits (152), Expect = 5e-11
Identities = 42/175 (24%), Positives = 80/175 (45%), Gaps = 10/175 (5%)
Frame = +1
Query: 226 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGA 405
+++W + P+ ++DIGK Y GD Y++L T + + W G++S ++
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDT 451
Query: 406 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGAQK 585
A L + + G VQ R G E F++ FQ P+ L+GG SG+ + + +
Sbjct: 452 AIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQ-PMVVLKGGLSSGYKSSMGESESTD 510
Query: 586 RMF--------QVKGK--RDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVGXKA 720
+ QV G + + QV+ S+N + F+L ++++ G ++
Sbjct: 511 ETYTPESIALVQVSGTGVHNNKAVQVETVATSLNSYECFLLQSGTSMFLWHGNQS 565
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 179 bits (436), Expect = 2e-45
Identities = 88/185 (47%), Positives = 119/185 (64%)
Frame = +1
Query: 193 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 372
PAF VG+ G +IWRI+NFEP+PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 373 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 552
G++++QDE+G AA+ TV LD GG AVQ+RE GHES FLSYF+ + LEGG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Query: 553 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVGXKAKNVE 732
+ R++ KGKR V ++QV +S+N DVF+LD IY F G + E
Sbjct: 130 KK-PEEEEFETRLYTCKGKRAVHLKQVPFARSSLNHDDVFILDTKEKIYQFNGANSNIQE 188
Query: 733 XLKAI 747
KA+
Sbjct: 189 RAKAL 193
Score = 67.7 bits (158), Expect = 9e-12
Identities = 44/172 (25%), Positives = 78/172 (45%), Gaps = 10/172 (5%)
Frame = +1
Query: 226 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGA 405
+++W I +++ +GK Y GD Y++L T + + W G+ S Q++
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 406 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGAQK 585
A L + + G VQ R G E F++ FQ + L+GG SG+ + +T G+
Sbjct: 454 AVRLASTMTNSLKGRPVQARIFEGKEPPQFVALFQ-HMVVLKGGLSSGYKNSMTEKGSSG 512
Query: 586 R--------MFQVKGK--RDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVG 711
+ QV G + + QV+ S+N D F+L ++++VG
Sbjct: 513 ETYTPESIALIQVSGTGVHNNKALQVEAVATSLNSYDCFLLQSGTSMFLWVG 564
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 177 bits (432), Expect = 6e-45
Identities = 85/185 (45%), Positives = 119/185 (64%)
Frame = +1
Query: 193 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 372
PAF G+ AG++IWRI+NF P P+ + IGKF+ GDSYI+L+TT+ L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 373 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 552
G++++QDE+G AA+ TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGF 129
Query: 553 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVGXKAKNVE 732
HVV R+F +GK V V++V +S+N D+++LD + I+ F G + E
Sbjct: 130 KHVVAEEHI-TRLFVCRGKHVVHVKEVPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQE 188
Query: 733 XLKAI 747
KA+
Sbjct: 189 RAKAL 193
Score = 55.6 bits (128), Expect = 4e-08
Identities = 40/175 (22%), Positives = 71/175 (40%), Gaps = 10/175 (5%)
Frame = +1
Query: 217 TAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDE 396
T +Q+WR+ + D KFY GD Y + + + I W G++S ++E
Sbjct: 391 TGNLQVWRVNGQAKTLLQAADHSKFYSGDCY-VFQYSYPGEEKEEVLIGTWFGKQSVEEE 449
Query: 397 SGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNA- 573
G+A + + + Q R G E F Q+ + + +GG SG+ +
Sbjct: 450 RGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGISSGYKKYIAEKE 508
Query: 574 ---------GAQKRMFQVKGKRDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVG 711
G Q G +++ QVDP AS+N ++L + ++ + G
Sbjct: 509 VDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAG 563
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 115 bits (276), Expect = 4e-26
Identities = 60/187 (32%), Positives = 101/187 (54%)
Frame = +1
Query: 187 VHPAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHY 366
+ AF VG +G++IW + N + I + + GKF+ G++Y++LRT + +DIHY
Sbjct: 8 IDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESPQYDIHY 67
Query: 367 WIGRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPS 546
W+G ++ + +S A+ + LD G VQ+RE G E+ FLSYF+ + +EG
Sbjct: 68 WLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEGKYSP 127
Query: 547 GFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVGXKAKN 726
+ Q + + KG VRV++V +S+N DVF+LD + +++F G +
Sbjct: 128 KTG--IAGETYQVTLLRCKGDHVVRVKEVPFLRSSLNHDDVFILDTASKVFLFAGCNSST 185
Query: 727 VEXLKAI 747
E KA+
Sbjct: 186 QEKAKAM 192
Score = 34.7 bits (76), Expect = 0.075
Identities = 36/174 (20%), Positives = 73/174 (41%), Gaps = 12/174 (6%)
Frame = +1
Query: 226 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILR--TTSDNRNNLSWDIHYWIGRESTQDES 399
+++WR+ + ++ D K + GD Y++ T + +L ++ WIG ES Q +
Sbjct: 391 LKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHL---LYVWIGCESIQQDR 447
Query: 400 GAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGA 579
A + G +V G+E + F FQ+ L +GG + ++
Sbjct: 448 ADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQS-LVVFKGGLSRRYKVLLAEKEK 506
Query: 580 --------QKRMFQVKG--KRDVRVRQVDPXIASMNKGDVFVLDLXNDIYVFVG 711
+ +F+V G R+++ QV+ S+N ++L + ++G
Sbjct: 507 IGEEYNENKASLFRVVGTSPRNMQAIQVNLVATSLNSSYSYILQYGASAFTWIG 560
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 28.3 bits (60), Expect = 6.5
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -2
Query: 242 ILHICTPAVLPTLAKAGWTLALSRAFLSDSDVIG 141
IL +C P +PTLA +T +L+DS +G
Sbjct: 113 ILPVCIPNRIPTLALTNYTQTGYARYLNDSRYVG 146
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,774,915
Number of Sequences: 28952
Number of extensions: 360961
Number of successful extensions: 965
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 958
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1892353600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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