Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_O09
(835 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 297 1e-81
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 291 8e-80
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 140 2e-34
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 119 4e-28
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 1.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.6
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 26 7.6
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 297 bits (729), Expect = 1e-81
Identities = 133/215 (61%), Positives = 159/215 (73%)
Frame = +1
Query: 109 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 288
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 289 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 468
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 469 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVXXX 648
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQV
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 649 XXXXXXXXXXXXXXXXXXDCAFMVDNEAIYDICRR 753
DC FMVDNE+ YDICRR
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Score = 39.5 bits (88), Expect = 6e-04
Identities = 18/25 (72%), Positives = 20/25 (80%)
Frame = +2
Query: 758 LDIERPTYTNLNRLIGXIVSSXTXS 832
LDIERP+Y NLNRLI +VSS T S
Sbjct: 217 LDIERPSYENLNRLIAQVVSSITAS 241
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 291 bits (714), Expect = 8e-80
Identities = 135/220 (61%), Positives = 161/220 (73%), Gaps = 5/220 (2%)
Frame = +1
Query: 109 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 273
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 274 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 453
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 454 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 633
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 634 QVXXXXXXXXXXXXXXXXXXXXXDCAFMVDNEAIYDICRR 753
QV DC FMVDNEA YDICRR
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Score = 41.1 bits (92), Expect = 2e-04
Identities = 19/25 (76%), Positives = 20/25 (80%)
Frame = +2
Query: 758 LDIERPTYTNLNRLIGXIVSSXTXS 832
LDIERPTY NLNRLI +VSS T S
Sbjct: 221 LDIERPTYENLNRLIAQVVSSITAS 245
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 140 bits (340), Expect = 2e-34
Identities = 73/212 (34%), Positives = 105/212 (49%)
Frame = +1
Query: 109 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 288
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 289 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 468
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 469 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVXXX 648
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 649 XXXXXXXXXXXXXXXXXXDCAFMVDNEAIYDI 744
D F +DNEA+ I
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSI 210
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 119 bits (287), Expect = 4e-28
Identities = 72/228 (31%), Positives = 111/228 (48%), Gaps = 3/228 (1%)
Frame = +1
Query: 112 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 291
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 292 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 465
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 466 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VX 642
D I + AD L+GF + H L+ERL+ Y KK ++++P Q V
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 643 XXXXXXXXXXXXXXXXXXXXDCAFMVDNEAIYDICRRXSRH*APNLHQ 786
D ++DN A+ I P HQ
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQ 227
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.9 bits (59), Expect = 1.9
Identities = 41/186 (22%), Positives = 70/186 (37%)
Frame = +2
Query: 188 STASSLMARCPQTRPSGVETILSTLSSARPELASTYPVXXXXXXXXXXXXXXXXAHTDSC 367
ST SSL + ++PS T ST SSA P S+ + + S
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS----SYLSSSSVVSSSSSPSSSSSS 228
Query: 368 FIQNNLLLVRKMRXXXXXXXXXXLERKS*IWFWTESASSLTSVPVCKDS*SSTPSVEVPA 547
+ ++ L + S T S+SS +S + S SS+ +
Sbjct: 229 TLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSS 288
Query: 548 LGSLPY*WSVSPLTTARSLNWSSPSTPRLRFHCRRRALQLYPHHPHNPXSTLTVLSWSTM 727
S S SP +T+ +++ SS S+ ++ +P S+ + +S S+
Sbjct: 289 TISSSSSSSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSS 348
Query: 728 KPSMTS 745
PS +S
Sbjct: 349 SPSSSS 354
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.6
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 207 WPDAHRQDHRGWRRFFQHFLQR 272
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 25.8 bits (54), Expect = 7.6
Identities = 26/95 (27%), Positives = 41/95 (43%), Gaps = 2/95 (2%)
Frame = +2
Query: 467 TESASSLTSVPVCKDS*--SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSTPRLRF 640
T S + TS P+ S SSTP L S +P+T++ +LN S+P T
Sbjct: 625 TSSTALNTSTPITSSSVLNSSTPITSSSVLNSS------TPITSSTALNTSTPITSSSVL 678
Query: 641 HCRRRALQLYPHHPHNPXSTLTVLSWSTMKPSMTS 745
+ + P ++ +VL+ ST S T+
Sbjct: 679 NSSTPITSSTALNTSTPITSSSVLNSSTAITSSTA 713
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,413,448
Number of Sequences: 5004
Number of extensions: 71292
Number of successful extensions: 228
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 212
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 410448950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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