Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_O02
(821 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 4e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 8e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 2e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 2e-29
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.4
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.2
SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 27 3.2
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.3
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.6
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.4
SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr ... 25 9.8
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 9.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 235 bits (576), Expect = 4e-63
Identities = 101/132 (76%), Positives = 117/132 (88%)
Frame = +2
Query: 62 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 241
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 242 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 421
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 422 RKEAESCDCLQG 457
R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132
Score = 161 bits (392), Expect = 8e-41
Identities = 74/100 (74%), Positives = 86/100 (86%)
Frame = +3
Query: 522 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 701
KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFAN 213
Query: 702 TLKLSTPTYGDLNHLVSLTMSGVTTCXRFPGQLNADLRKL 821
TLK+ +P+Y DLNHLVS M+GVTT RFPG+LN+DLRKL
Sbjct: 214 TLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKL 253
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 8e-33
Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%)
Frame = +2
Query: 62 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 235
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 236 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 415
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 416 VVRKEAESCDCLQG 457
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 93.9 bits (223), Expect = 2e-20
Identities = 41/97 (42%), Positives = 62/97 (63%)
Frame = +3
Query: 522 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 701
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
Query: 702 TLKLSTPTYGDLNHLVSLTMSGVTTCXRFPGQLNADL 812
L + P+Y +LN L++ +S +T RF G LN DL
Sbjct: 216 NLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDL 252
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (314), Expect = 2e-31
Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%)
Frame = +2
Query: 62 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 223
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 224 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 403
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 404 SVLDVVRKEAESCDCLQG 457
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 100 bits (240), Expect = 2e-22
Identities = 44/99 (44%), Positives = 65/99 (65%)
Frame = +3
Query: 522 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFR 701
++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
Query: 702 TLKLSTPTYGDLNHLVSLTMSGVTTCXRFPGQLNADLRK 818
L + PTY +LN L++ +S +T RF G LN DL +
Sbjct: 220 NLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNE 258
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 124 bits (299), Expect = 2e-29
Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%)
Frame = +2
Query: 65 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 244
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 245 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 418
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 419 VRKEAESCDCLQG 457
+ +EA+ D L+G
Sbjct: 122 IDREADGSDSLEG 134
Score = 85.8 bits (203), Expect = 7e-18
Identities = 43/98 (43%), Positives = 57/98 (58%), Gaps = 1/98 (1%)
Frame = +3
Query: 522 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICF 698
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215
Query: 699 RTLKLSTPTYGDLNHLVSLTMSGVTTCXRFPGQLNADL 812
L PT+ N LVS MS TT R+PG +N DL
Sbjct: 216 DRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDL 253
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.4
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 365 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 463
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.2
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 101 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 202
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPBC56F2.07c |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 809
Score = 27.1 bits (57), Expect = 3.2
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -2
Query: 820 SLRRSAFSCPGNRXHVVTPDMVSDTRWFRSPYVGVDSFSVRKQI 689
+LR NR ++ P ++ R R YVG +F RKQI
Sbjct: 679 ALRNVLVLAATNRPDMIDPALMRPGRLDRLLYVGPPNFEARKQI 722
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 296 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 397
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 5.6
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 438 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 301
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 7.4
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 317 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 448
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 391
Score = 25.4 bits (53), Expect = 9.8
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +3
Query: 735 LNHLVSLTMSGVTTCXRFPGQLN 803
L HLV + G++ C +PG+ N
Sbjct: 331 LTHLVDAPLLGMSVCPLYPGEEN 353
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 9.8
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -3
Query: 354 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 259
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,137,843
Number of Sequences: 5004
Number of extensions: 61412
Number of successful extensions: 200
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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