Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_N14
(873 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 100 2e-22
SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 71 2e-13
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 4.6
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 26 6.1
SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p... 26 6.1
SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|... 26 6.1
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 8.1
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 8.1
>SPBC4C3.07 |||translation initiation factor
eIF3f|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 100 bits (240), Expect = 2e-22
Identities = 58/176 (32%), Positives = 95/176 (53%), Gaps = 15/176 (8%)
Frame = +2
Query: 23 ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVE 196
+++ + P VLF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE
Sbjct: 21 LNIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVE 80
Query: 197 AELNYAMDVYELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREP---------- 346
E+ Y +Y L+ + N E +VGW+AT ++ S++I Y+ EP
Sbjct: 81 VEMEYHRAMYHLHLKANPREVVVGWYATSPDLDAFSALIQNLYASPA-EPGTAPLGTYPH 139
Query: 347 --VHVTLDTSLAGGRMGLRAYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 505
VH+T++T ++ + ++ YV P+G+ C F P T+ E GL+
Sbjct: 140 PCVHLTVNTDVS-SPLAIKTYVSSPVGITERLADSCAFVPTPFTIRDDEAVRSGLK 194
>SPCC1682.10 |rpn8||19S proteasome regulatory subunit
Rpn8|Schizosaccharomyces pombe|chr 3|||Manual
Length = 324
Score = 71.3 bits (167), Expect = 2e-13
Identities = 44/164 (26%), Positives = 77/164 (46%), Gaps = 5/164 (3%)
Frame = +2
Query: 29 VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA-- 199
V VHP+VL VD+Y R RV+G LLG ++ VV V N + +P +E
Sbjct: 17 VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWF 76
Query: 200 -ELNYAMDVYELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREPVHVTLDTSLA 376
+ N+ + E+ +++N++E +VGW+ TG ++ I+ + PV V +D
Sbjct: 77 LDHNFMESMNEMFKKINANEKLVGWYHTGPQLRPSDLEINNLLKKYIPNPVLVIIDVKPK 136
Query: 377 GGRMGLRAYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 505
+ AY + +G K F + ++ E E +G++
Sbjct: 137 SVGLPTNAYFAIDEIEDDGSKSSRTFVHLPSSIEAEEAEEIGVE 180
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 26.6 bits (56), Expect = 4.6
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = +2
Query: 71 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 196
Y+ R DSHR I L GT + + + + +K H ++E
Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = +1
Query: 7 KNGSQY*CEGPSCCFISNCGRIRTSKCRF 93
K Q+ + P C IS R SKCR+
Sbjct: 1951 KQAPQFQSKSPECVIISTMNRSCDSKCRY 1979
>SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 485
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/62 (25%), Positives = 31/62 (50%), Gaps = 5/62 (8%)
Frame = +2
Query: 17 LNISVKVHPVVLFQIVDAYERRNADSHRVI-----GTLLGTSDKGVVEVTNCFCVPHKEH 181
LNI ++ +V+ ++++ ++RN D H I + TSD ++ + N F + H
Sbjct: 253 LNIIIQEVDIVIGELIEGLKKRNIDKHVNIIFLSDHGMAPTSDNRLIWLDNMFNLSAVAH 312
Query: 182 AD 187
D
Sbjct: 313 RD 314
>SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 402
Score = 26.2 bits (55), Expect = 6.1
Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = -3
Query: 532 STRHSLLAHL*TYNLRFIACESDI--YRSEHATLLSIWHSQWYTNICT*THSTSSQRSVQ 359
ST HSLL+++ + + IACE YR + + W + + C ++ +Q Q
Sbjct: 307 STIHSLLSYVFSRQTQNIACEPMYIDYRKDETEAIIRWKTPLHAETC--INAFRTQERKQ 364
Query: 358 SNMDRLTA 335
++ D + A
Sbjct: 365 NSHDDIRA 372
>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = -3
Query: 454 SEHATLLSIWHSQWYTNICT*THSTSSQRSVQSNMDRLTAFTGI 323
S A +L + W+ N+ T S+RS+ +N D +T+F+ +
Sbjct: 410 SAKARILDSFCIDWHLNV-----QTMSERSILTNRDFITSFSAV 448
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 25.8 bits (54), Expect = 8.1
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +1
Query: 82 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 225
+ R S SY I ++ SGG + + L A RTC S + T L + CL
Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,939,343
Number of Sequences: 5004
Number of extensions: 58286
Number of successful extensions: 161
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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