Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_N10
(844 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 279 3e-76
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 272 5e-74
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 142 7e-35
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 123 4e-29
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 279 bits (685), Expect = 3e-76
Identities = 122/179 (68%), Positives = 147/179 (82%)
Frame = +2
Query: 128 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVF 307
HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++
Sbjct: 8 HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67
Query: 308 VDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLAD 487
VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD
Sbjct: 68 VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
Query: 488 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYN 664
C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYN
Sbjct: 128 NCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYN 186
Score = 48.4 bits (110), Expect = 1e-06
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = +1
Query: 688 LEHSDCAFMVDNEAIYDICRRXLDIER 768
L+ +DC FMVDNE+ YDICRR LDIER
Sbjct: 195 LDLADCTFMVDNESCYDICRRNLDIER 221
Score = 29.5 bits (63), Expect = 0.62
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +3
Query: 768 PTYTXLNRLIGQIVSSXTXS 827
P+Y LNRLI Q+VSS T S
Sbjct: 222 PSYENLNRLIAQVVSSITAS 241
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 272 bits (666), Expect = 5e-74
Identities = 122/184 (66%), Positives = 149/184 (80%), Gaps = 5/184 (2%)
Frame = +2
Query: 128 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHV 292
HVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSETG GK V
Sbjct: 8 HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66
Query: 293 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 472
PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 473 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 652
R++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAPQVST+VV
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV 186
Query: 653 EPYN 664
EPYN
Sbjct: 187 EPYN 190
Score = 52.8 bits (121), Expect = 6e-08
Identities = 24/30 (80%), Positives = 26/30 (86%)
Frame = +1
Query: 688 LEHSDCAFMVDNEAIYDICRRXLDIERQPT 777
L++SDC FMVDNEA YDICRR LDIER PT
Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIER-PT 227
Score = 31.1 bits (67), Expect = 0.20
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +3
Query: 768 PTYTXLNRLIGQIVSSXTXS 827
PTY LNRLI Q+VSS T S
Sbjct: 226 PTYENLNRLIAQVVSSITAS 245
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 142 bits (343), Expect = 7e-35
Identities = 70/177 (39%), Positives = 98/177 (55%)
Frame = +2
Query: 134 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 313
GQ G Q+G A W EHG+ G T + N +F+E GK+VPRAV VD
Sbjct: 10 GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67
Query: 314 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 493
LEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 494 TGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYN 664
LQGF + H LL+ ++ +Y + F++ PAP+ S VVEPYN
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYN 184
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 123 bits (296), Expect = 4e-29
Identities = 67/180 (37%), Positives = 102/180 (56%), Gaps = 3/180 (1%)
Frame = +2
Query: 134 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 313
GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +++PRA+ +D
Sbjct: 11 GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68
Query: 314 LEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLAD 487
LEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 488 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYN 664
L+GF + H L+ERL+ Y KK ++++P Q VS VV+PYN
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 186 STASSLMARCPQTRPSGVETILSTLSSARPELAST 290
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 205 WPDAHRQDHRGWRRFFQHFLQR 270
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,229,281
Number of Sequences: 5004
Number of extensions: 64905
Number of successful extensions: 178
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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