Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_M15
(851 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 256 4e-69
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 248 6e-67
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 126 4e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 111 9e-26
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 32 0.090
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 27 2.6
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 5.9
SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor |Schizosacc... 26 5.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 256 bits (626), Expect = 4e-69
Identities = 113/173 (65%), Positives = 136/173 (78%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 283
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 284 HVPRAVFVGLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
+VPR+++V LEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFXXLP 622
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F P
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYP 173
Score = 72.1 bits (169), Expect = 9e-14
Identities = 31/44 (70%), Positives = 35/44 (79%)
Frame = +3
Query: 618 YPAPQVSTAVVEPYNSIXTTXTTLEHSDCASMVDNEXIYDXCRR 749
YPAPQVST+VVEPYNS+ TT TL+ +DC MVDNE YD CRR
Sbjct: 172 YPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 248 bits (608), Expect = 6e-67
Identities = 114/178 (64%), Positives = 138/178 (77%), Gaps = 5/178 (2%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 268
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 269 TGAGKHVPRAVFVGLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 448
TG GK VPR+++V LEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 449 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFXXLP 622
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F P
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYP 177
Score = 74.9 bits (176), Expect = 1e-14
Identities = 32/44 (72%), Positives = 36/44 (81%)
Frame = +3
Query: 618 YPAPQVSTAVVEPYNSIXTTXTTLEHSDCASMVDNEXIYDXCRR 749
YPAPQVST+VVEPYNS+ TT TL++SDC MVDNE YD CRR
Sbjct: 176 YPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 126 bits (304), Expect = 4e-30
Identities = 63/173 (36%), Positives = 90/173 (52%)
Frame = +2
Query: 104 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 283
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 284 HVPRAVFVGLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 463
+VPRAV V LEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 464 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFXXLP 622
+R+ A+ C LQGF + H LL+ ++ +Y + F P
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAP 171
Score = 36.3 bits (80), Expect = 0.006
Identities = 16/37 (43%), Positives = 23/37 (62%)
Frame = +3
Query: 621 PAPQVSTAVVEPYNSIXTTXTTLEHSDCASMVDNEXI 731
PAP+ S VVEPYN+ + +E+SD +DNE +
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 111 bits (268), Expect = 9e-26
Identities = 61/176 (34%), Positives = 95/176 (53%), Gaps = 2/176 (1%)
Frame = +2
Query: 107 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 286
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 287 VPRAVFVGLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 460
+PRA+ + LEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 461 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFXXLPRA 628
D I + AD L+GF + H L+ERL+ Y KK + P +
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNS 175
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +3
Query: 618 YPAPQ-VSTAVVEPYNSIXTTXTTLEHSDCASMVDN 722
+P Q VS VV+PYNS+ ++D ++DN
Sbjct: 172 FPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDN 207
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 32.3 bits (70), Expect = 0.090
Identities = 22/81 (27%), Positives = 35/81 (43%)
Frame = +3
Query: 468 SASSLTSVPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSXXYPAPQVSTAV 647
S++ +S P+ S S T S +P G+ S +P+T SS +T+
Sbjct: 377 SSTGTSSSPLLSTSTSCTTSTSIPPTGN-----STTPVTPTVPPTSSSTPLTTTNCTTST 431
Query: 648 VEPYNSIXTTXTTLEHSDCAS 710
PY S T T L ++C +
Sbjct: 432 SVPYTSTPVTSTPLATTNCTT 452
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 27.5 bits (58), Expect = 2.6
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = -3
Query: 363 NNCLYVPVRTSSTTVGSRPTKTARGTCLPAPVSLKKVLKESSPP 232
N+ +Y PV +SSTT S T + T A S + +S P
Sbjct: 740 NDAVYYPVSSSSTTTTSTSTGSVTTTSTTATASCTLPISYTSTP 783
Score = 26.6 bits (56), Expect = 4.5
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = -3
Query: 369 G*NNCLYVPVRTSSTTVGSRPTKTARGTCLPAPVS 265
G +N PV++SSTT PT T + P P +
Sbjct: 832 GCDNGALGPVQSSSTTSSITPTPTTTSSITPTPTT 866
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/34 (35%), Positives = 16/34 (47%)
Frame = +2
Query: 575 SVDYGKKSKLEFXXLPRASGFHCRRRALQLYXHH 676
+ D S F +P+A GFH RR A + H
Sbjct: 1305 TTDVDSSSASFFLEIPKALGFHRRREAQKFRNQH 1338
>SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 539
Score = 26.2 bits (55), Expect = 5.9
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 4/47 (8%)
Frame = -3
Query: 318 GSRPTKTARGTCLPAPV----SLKKVLKESSPPPMVLSVGIWPSGWM 190
G +P K A+ + LP PV S K++ K P L+V ++ SG M
Sbjct: 35 GKKPVKRAKVSSLPKPVRVPGSAKRINKIPELPTERLNVYVFGSGSM 81
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 183 STASSLMARCPQTRPSGVETILSTLSSARPELAST 287
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 202 WPDAHRQDHRGWRRFFQHFLQR 267
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,052,931
Number of Sequences: 5004
Number of extensions: 62339
Number of successful extensions: 187
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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