Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_M08
         (819 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   292   3e-80
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   285   5e-78
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   150   3e-37
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   131   1e-31
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.4  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.4  
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom...    25   9.8  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  292 bits (717), Expect = 3e-80
 Identities = 128/188 (68%), Positives = 155/188 (82%)
 Frame = +2

Query: 98  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 277
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 278 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 457
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 458 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 637
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 638 VVEPYNSI 661
           VVEPYNS+
Sbjct: 181 VVEPYNSV 188



 Score = 66.5 bits (155), Expect = 4e-12
 Identities = 30/42 (71%), Positives = 33/42 (78%)
 Frame = +3

Query: 690 SDCAFMVDNEAIYDICRRNLDIERPTYXT*XXLIGQXVSSIT 815
           +DC FMVDNE+ YDICRRNLDIERP+Y     LI Q VSSIT
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSIT 239


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  285 bits (699), Expect = 5e-78
 Identities = 129/193 (66%), Positives = 157/193 (81%), Gaps = 5/193 (2%)
 Frame = +2

Query: 98  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 262
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 263 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 442
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 443 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 622
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 623 QVSTAVVEPYNSI 661
           QVST+VVEPYNS+
Sbjct: 180 QVSTSVVEPYNSV 192



 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 33/43 (76%), Positives = 34/43 (79%)
 Frame = +3

Query: 687 HSDCAFMVDNEAIYDICRRNLDIERPTYXT*XXLIGQXVSSIT 815
           +SDC FMVDNEA YDICRRNLDIERPTY     LI Q VSSIT
Sbjct: 201 NSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSIT 243


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  150 bits (363), Expect = 3e-37
 Identities = 73/187 (39%), Positives = 104/187 (55%)
 Frame = +2

Query: 98  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 277
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 278 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 457
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 458 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 637
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 638 VVEPYNS 658
           VVEPYN+
Sbjct: 179 VVEPYNA 185



 Score = 31.1 bits (67), Expect = 0.20
 Identities = 14/44 (31%), Positives = 24/44 (54%)
 Frame = +3

Query: 684 KHSDCAFMVDNEAIYDICRRNLDIERPTYXT*XXLIGQXVSSIT 815
           ++SD  F +DNEA+  I    L I+ P+Y     L+   ++ +T
Sbjct: 194 ENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVT 237


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  131 bits (317), Expect = 1e-31
 Identities = 71/190 (37%), Positives = 109/190 (57%), Gaps = 3/190 (1%)
 Frame = +2

Query: 101 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 280
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 281 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 454
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 455 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 631
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 632 TAVVEPYNSI 661
             VV+PYNS+
Sbjct: 180 DVVVQPYNSL 189


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 177 STASSLMARCPQTRPSGVETILSTLSSARPELAST 281
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 196 WPDAHRQDHRGWRRFFQHFLQR 261
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 534

 Score = 25.4 bits (53), Expect = 9.8
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = +3

Query: 87  LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 209
           L+S        +L+ P SR++ P  S +  STASSL    P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,261,588
Number of Sequences: 5004
Number of extensions: 66472
Number of successful extensions: 179
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)