Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_L24
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 280 2e-76
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 275 4e-75
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 141 1e-34
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 113 2e-26
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 27 4.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 280 bits (687), Expect = 2e-76
Identities = 127/204 (62%), Positives = 156/204 (76%)
Frame = +1
Query: 178 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 357
CLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR
Sbjct: 25 CLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYR 84
Query: 358 QLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXX 537
LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 85 DLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGG 144
Query: 538 XXXXXXXLLMERLSVDYGKKSELEFAIYPAPQVSTAVVEPYNSIXXXXXXXXXXXXCFSW 717
LL+ERL+++Y KKS+L+F++YPAPQVST+VVEPYNS+ C
Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSV-LTTHATLDLADCTFM 203
Query: 718 VDNEAIYDICRRNLDIERPTYTNL 789
VDNE+ YDICRRNLDIERP+Y NL
Sbjct: 204 VDNESCYDICRRNLDIERPSYENL 227
Score = 52.0 bits (119), Expect = 1e-07
Identities = 21/24 (87%), Positives = 22/24 (91%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACWELY 176
MRE IS+HVGQAG QIGNACWELY
Sbjct: 1 MREIISIHVGQAGTQIGNACWELY 24
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 275 bits (675), Expect = 4e-75
Identities = 129/209 (61%), Positives = 157/209 (75%), Gaps = 5/209 (2%)
Frame = +1
Query: 178 CLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVR 342
CLEHGI PDG PT+ K +D F TFFSETG GK VPR+++VDLEP V+D+VR
Sbjct: 25 CLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVR 83
Query: 343 TGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 522
TG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 84 TGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFH 143
Query: 523 XXXXXXXXXXXXLLMERLSVDYGKKSELEFAIYPAPQVSTAVVEPYNSIXXXXXXXXXXX 702
LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+
Sbjct: 144 SFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSV-LTTHATLDNS 202
Query: 703 XCFSWVDNEAIYDICRRNLDIERPTYTNL 789
C VDNEA YDICRRNLDIERPTY NL
Sbjct: 203 DCTFMVDNEACYDICRRNLDIERPTYENL 231
Score = 54.4 bits (125), Expect = 2e-08
Identities = 27/44 (61%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACWELY-LPGARHPA*WPDAHRQDHR 233
MRE ISVHVGQAGVQIGNACWELY L P +P + + H+
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHK 44
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 141 bits (341), Expect = 1e-34
Identities = 74/202 (36%), Positives = 107/202 (52%)
Frame = +1
Query: 184 EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQL 363
EHG+ G T + N +F+E GK+VPRAV VDLEP +D V++G + L
Sbjct: 27 EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84
Query: 364 FHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 543
F P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144
Query: 544 XXXXXLLMERLSVDYGKKSELEFAIYPAPQVSTAVVEPYNSIXXXXXXXXXXXXCFSWVD 723
LL+ ++ +Y + F++ PAP+ S VVEPYN+ F +D
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFC-ID 203
Query: 724 NEAIYDICRRNLDIERPTYTNL 789
NEA+ I L I+ P+Y +L
Sbjct: 204 NEALSSIFANTLKIKSPSYDDL 225
Score = 27.5 bits (58), Expect = 2.5
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACW 167
MRE + + GQ G Q+G A W
Sbjct: 1 MREIVHIQAGQCGNQVGAAFW 21
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 113 bits (273), Expect = 2e-26
Identities = 68/204 (33%), Positives = 106/204 (51%), Gaps = 3/204 (1%)
Frame = +1
Query: 178 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 357
CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP VV+ + + TY
Sbjct: 26 CLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83
Query: 358 QLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 531
L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 532 XXXXXXXXXLLMERLSVDYGKKSELEFAIYPAPQ-VSTAVVEPYNSIXXXXXXXXXXXXC 708
L+ERL+ Y KK ++++P Q VS VV+PYNS+
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSV 202
Query: 709 FSWVDNEAIYDICRRNLDIERPTY 780
+DN A+ I L + PT+
Sbjct: 203 VV-LDNAALAHIAADRLHTQNPTF 225
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 26.6 bits (56), Expect = 4.4
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +1
Query: 649 VEPYNSIXXXXXXXXXXXXCFSWVDNEAIYDICRRNLDIERP 774
V+P N+I +WV N + D C R+L+I P
Sbjct: 598 VDPVNTIEDTSQEIGFFNASLTWVSNPSPGDFCLRDLNIVFP 639
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 5.9
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +2
Query: 176 LAWSTASSLMARCPQTRPSGVETILSTLSSARPELAST 289
L ST SSL + ++PS T ST SSA P S+
Sbjct: 170 LTSSTFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,377,662
Number of Sequences: 5004
Number of extensions: 70118
Number of successful extensions: 199
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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