Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_L23
(852 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 240 2e-64
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 231 1e-61
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 123 4e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 100 3e-22
SPAC1805.05 |cki3||serine/threonine protein kinase Cki3|Schizosa... 28 1.9
SPBC215.08c |arg4||carbamoyl-phosphate synthase Arg4|Schizosacch... 28 1.9
SPBC32F12.02 |rec14||recombination protein Rec14|Schizosaccharom... 27 4.5
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 26 7.8
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 240 bits (587), Expect = 2e-64
Identities = 106/163 (65%), Positives = 131/163 (80%)
Frame = +2
Query: 167 ELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTDVDEVRTG 346
ELYCLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP +D+VRTG
Sbjct: 22 ELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTG 81
Query: 347 TYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXX 526
YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 82 PYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSF 141
Query: 527 XXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEP 655
LL+ERL+++Y KKSKL+F++YPAPQVST+VVEP
Sbjct: 142 GGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEP 184
Score = 62.9 bits (146), Expect = 6e-11
Identities = 30/63 (47%), Positives = 37/63 (58%)
Frame = +3
Query: 657 YNSILTTHTXLXXSDCASMVDXEAIYXXCRRXSRX*APTYTNLNXSSXXIVSXIXXSLXF 836
YNS+LTTH L +DC MVD E+ Y CRR P+Y NLN +VS I SL F
Sbjct: 185 YNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRF 244
Query: 837 DGA 845
+G+
Sbjct: 245 EGS 247
Score = 45.6 bits (103), Expect = 9e-06
Identities = 18/21 (85%), Positives = 19/21 (90%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACW 167
MRE IS+HVGQAG QIGNACW
Sbjct: 1 MREIISIHVGQAGTQIGNACW 21
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 231 bits (564), Expect = 1e-61
Identities = 105/168 (62%), Positives = 132/168 (78%), Gaps = 5/168 (2%)
Frame = +2
Query: 167 ELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTDVD 331
ELYCLEHGI PDG PT+ K +D F TFFSETG GK VPR+++VDLEP +D
Sbjct: 22 ELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVID 80
Query: 332 EVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFL 511
+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL
Sbjct: 81 QVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFL 140
Query: 512 IFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEP 655
+FH LL+ERL+++YGKKS L+F++YPAPQVST+VVEP
Sbjct: 141 VFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEP 188
Score = 65.3 bits (152), Expect = 1e-11
Identities = 33/63 (52%), Positives = 36/63 (57%)
Frame = +3
Query: 657 YNSILTTHTXLXXSDCASMVDXEAIYXXCRRXSRX*APTYTNLNXSSXXIVSXIXXSLXF 836
YNS+LTTH L SDC MVD EA Y CRR PTY NLN +VS I SL F
Sbjct: 189 YNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRF 248
Query: 837 DGA 845
G+
Sbjct: 249 AGS 251
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/21 (95%), Positives = 20/21 (95%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACW 167
MRE ISVHVGQAGVQIGNACW
Sbjct: 1 MREVISVHVGQAGVQIGNACW 21
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 123 bits (296), Expect = 4e-29
Identities = 61/158 (38%), Positives = 88/158 (55%)
Frame = +2
Query: 182 EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTDVDEVRTGTYRQL 361
EHG+ G T + N +F+E GK+VPRAV VDLEP +D V++G + L
Sbjct: 27 EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84
Query: 362 FHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 541
F P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144
Query: 542 XXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEP 655
LL+ ++ +Y + F++ PAP+ S VVEP
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEP 182
Score = 31.9 bits (69), Expect = 0.12
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +3
Query: 657 YNSILTTHTXLXXSDCASMVDXEAIYXXCRRXSRX*APTYTNLNXSSXXIVSXIXXSLXF 836
YN+ L+ H + SD +D EA+ + +P+Y +LN +++ + S F
Sbjct: 183 YNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRF 242
Query: 837 DG 842
G
Sbjct: 243 PG 244
Score = 29.5 bits (63), Expect = 0.63
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACWSFTA 179
MRE + + GQ G Q+G A WS A
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIA 25
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 100 bits (239), Expect = 3e-22
Identities = 57/163 (34%), Positives = 90/163 (55%), Gaps = 3/163 (1%)
Frame = +2
Query: 176 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTDVDEVRTGTYR 355
CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP V+ + + TY
Sbjct: 26 CLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83
Query: 356 QLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 529
L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 530 XXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEP 655
L+ERL+ Y KK ++++P Q VS VV+P
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQP 185
>SPAC1805.05 |cki3||serine/threonine protein kinase
Cki3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 439
Score = 27.9 bits (59), Expect = 1.9
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = -2
Query: 182 PGSKAPAGITDLDSGLANVYRDALTH 105
PGSK I +D G+A YRD TH
Sbjct: 145 PGSKTENVIYAVDFGMAKQYRDPKTH 170
>SPBC215.08c |arg4||carbamoyl-phosphate synthase
Arg4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1160
Score = 27.9 bits (59), Expect = 1.9
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 117 ISVHVGQAGVQIGNACWSFTAWSTASSLMARCPQ 218
+S HV AGV G+A + +S + +++RC +
Sbjct: 847 VSEHVENAGVHSGDATIALPPYSLSEDILSRCKE 880
>SPBC32F12.02 |rec14||recombination protein
Rec14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 26.6 bits (56), Expect = 4.5
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = -3
Query: 277 SSGLAEESVERIVSTPDGLVCGHLAIRLDA-VLQAVKLQQALPIWTP 140
S G AE S+ + TP L HLA LD V+ + Q + +W P
Sbjct: 42 SIGDAEHSLVEEIDTPHKLGVHHLATSLDENVVVSCGFGQDVYVWNP 88
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 25.8 bits (54), Expect = 7.8
Identities = 8/28 (28%), Positives = 20/28 (71%)
Frame = -3
Query: 397 IFLTSNKLFWMKQLSVCASADLINISRF 314
+F+T +K+FW + L + S+ ++++ +F
Sbjct: 284 LFITIHKIFWRQILGMGVSSFMVSVCQF 311
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 183 STASSLMARCPQTRPSGVETILSTLSSARPELAST 287
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 202 WPDAHRQDHRGWRRFFQHFLQR 267
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,135,485
Number of Sequences: 5004
Number of extensions: 61792
Number of successful extensions: 156
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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