Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_L16
(842 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g04780.1 68417.m00700 expressed protein very low similarity t... 35 0.059
At1g33450.1 68414.m04140 hypothetical protein low similarity to ... 35 0.078
At5g15690.1 68418.m01835 hypothetical protein very low similarit... 33 0.18
At4g24020.1 68417.m03452 RWP-RK domain-containing protein simila... 33 0.24
At1g44780.1 68414.m05130 expressed protein ; expression supporte... 30 1.7
At1g18520.1 68414.m02311 senescence-associated family protein si... 30 2.2
At2g41360.1 68415.m05105 F-box family protein similar to SKP1 in... 29 5.1
At4g14310.2 68417.m02205 peroxisomal membrane protein-related co... 28 6.8
At4g14310.1 68417.m02204 peroxisomal membrane protein-related co... 28 6.8
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil... 28 6.8
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot... 28 8.9
At2g04330.1 68415.m00429 hypothetical protein contains Pfam prof... 28 8.9
>At4g04780.1 68417.m00700 expressed protein very low similarity to
SP|Q13503 RNA polymerase II holoenzyme component SRB7
(RNAPII complex component SRB7) {Homo sapiens}
Length = 381
Score = 35.1 bits (77), Expect = 0.059
Identities = 21/78 (26%), Positives = 40/78 (51%), Gaps = 2/78 (2%)
Frame = +2
Query: 263 RHETENHALKCPFSRLIEDIFHIHNKTDKITQLKDREKTRLFLNTMAN--ITNSNSDKKQ 436
+ E E H K L+++I ++ K ++ + + L +N MAN + N N+ + +
Sbjct: 55 KRENEEHLFKWVDEALLDEIKMVNEKCKRVVE----NISDLRMNVMANMELLNKNAKQME 110
Query: 437 EELINDIIASINRIKENI 490
EELI + + +KEN+
Sbjct: 111 EELIKKMEGELLTMKENV 128
>At1g33450.1 68414.m04140 hypothetical protein low similarity to
MtN20 [Medicago truncatula] GI:2598591
Length = 160
Score = 34.7 bits (76), Expect = 0.078
Identities = 21/78 (26%), Positives = 39/78 (50%), Gaps = 2/78 (2%)
Frame = +2
Query: 263 RHETENHALKCPFSRLIEDIFHIHNKTDKITQLKDREKTRLFLNTMAN--ITNSNSDKKQ 436
+ E E H K L+++I ++ K ++ + + L +N MAN + N N + +
Sbjct: 55 KRENEEHLFKWVDEALLDEIKMVNEKCKRVAE----NISDLRMNVMANMELLNKNGKQME 110
Query: 437 EELINDIIASINRIKENI 490
EELI + + +KEN+
Sbjct: 111 EELIKKMEGELLTMKENV 128
>At5g15690.1 68418.m01835 hypothetical protein very low similarity
to MtN20 [Medicago truncatula] GI:2598591
Length = 169
Score = 33.5 bits (73), Expect = 0.18
Identities = 20/78 (25%), Positives = 39/78 (50%), Gaps = 2/78 (2%)
Frame = +2
Query: 263 RHETENHALKCPFSRLIEDIFHIHNKTDKITQLKDREKTRLFLNTMAN--ITNSNSDKKQ 436
+ E E H K L+++I ++ K ++ + + L +N M N + N N+ + +
Sbjct: 55 KRENEEHLFKWVDEALLDEIKMVNEKCKRVAE----NISDLRMNVMVNMELLNKNAKQME 110
Query: 437 EELINDIIASINRIKENI 490
EELI + + +KEN+
Sbjct: 111 EELIKKMEGELLTMKENV 128
>At4g24020.1 68417.m03452 RWP-RK domain-containing protein similar
to nodule inception protein [Lotus japonicus]
GI:6448579; contains Pfam profile: PF02042 RWP-RK domain
Length = 959
Score = 33.1 bits (72), Expect = 0.24
Identities = 25/78 (32%), Positives = 34/78 (43%)
Frame = +2
Query: 425 DKKQEELINDIIASINRIKENITKGNTGVINEAILLDKPIKVNQIKSGIEEKIDVQTKNQ 604
DKK I I + K N T E +L+ +P+ Q + EKI+V T N
Sbjct: 524 DKKVHSKIESIRVPFSGFKSNAT--------ETMLIPQPVV--QSSDPVNEKINVATVNG 573
Query: 605 TNKIKSSNERDKGKTTTT 658
K K E+ +GKT T
Sbjct: 574 VVKEKKKTEKKRGKTEKT 591
>At1g44780.1 68414.m05130 expressed protein ; expression supported
by MPSS
Length = 471
Score = 30.3 bits (65), Expect = 1.7
Identities = 26/117 (22%), Positives = 50/117 (42%), Gaps = 3/117 (2%)
Frame = +2
Query: 329 IHNKTDKITQLKDREKTR---LFLNTMANITNSNSDKKQEELINDIIASINRIKENITKG 499
I+ + D++ QL D K + N + ++ S E +D N E +
Sbjct: 191 INKELDEVLQLPDAPKCSTESIVKNVKKKVKSTPSKMVSSEYNSDSDTEGNVDNEEVAVK 250
Query: 500 NTGVINEAILLDKPIKVNQIKSGIEEKIDVQTKNQTNKIKSSNERDKGKTTTTERTK 670
T + + L KP + + KS +++ + K + +I S N+ D G + + +TK
Sbjct: 251 KT--MARKVKLSKPEMMGKRKSENGKQVSGRKKAKHTEIDSENDSDSGDSEKSLKTK 305
>At1g18520.1 68414.m02311 senescence-associated family protein
similar to senescence-associated protein 5 [Hemerocallis
hybrid cultivar] gi|3551954|gb|AAC34855
Length = 271
Score = 29.9 bits (64), Expect = 2.2
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = -3
Query: 498 PLVIFSLILFIEAIMSLISSSCFLSELLFVMLAIVLRNNLVFSLSFN 358
PL+ LILF+ +++ +I SCF L V I+L +V + F+
Sbjct: 46 PLLTTGLILFLVSLLGVI-GSCFKENLAMVSYLIILFGGIVALMIFS 91
>At2g41360.1 68415.m05105 F-box family protein similar to SKP1
interacting partner 6 [Arabidopsis thaliana]
GI:10716957; contains Pfam profile PF00646: F-box domain
Length = 373
Score = 28.7 bits (61), Expect = 5.1
Identities = 13/37 (35%), Positives = 25/37 (67%), Gaps = 2/37 (5%)
Frame = +2
Query: 386 FLNTMANIT--NSNSDKKQEELINDIIASINRIKENI 490
+++T N T N+N++KK+EE I + ++NR+ + I
Sbjct: 310 YISTSINTTKNNNNNNKKKEEKIWSALIALNRVGDGI 346
>At4g14310.2 68417.m02205 peroxisomal membrane protein-related
contains weak similarity to Peroxisomal membrane protein
2 (22 kDa peroxisomal membrane protein)
(Swiss-Prot:P42925) [Mus musculus]
Length = 965
Score = 28.3 bits (60), Expect = 6.8
Identities = 18/63 (28%), Positives = 30/63 (47%)
Frame = +2
Query: 437 EELINDIIASINRIKENITKGNTGVINEAILLDKPIKVNQIKSGIEEKIDVQTKNQTNKI 616
E + I + I + K+ + N ++ I+ D K+ I+ + ID KN+T K
Sbjct: 307 EGKVKKIASDIKKTKDMLDLNNPDS-SKVIISDIHQKITGIEKSMSHVIDGPEKNKTTKA 365
Query: 617 KSS 625
KSS
Sbjct: 366 KSS 368
>At4g14310.1 68417.m02204 peroxisomal membrane protein-related
contains weak similarity to Peroxisomal membrane protein
2 (22 kDa peroxisomal membrane protein)
(Swiss-Prot:P42925) [Mus musculus]
Length = 1087
Score = 28.3 bits (60), Expect = 6.8
Identities = 18/63 (28%), Positives = 30/63 (47%)
Frame = +2
Query: 437 EELINDIIASINRIKENITKGNTGVINEAILLDKPIKVNQIKSGIEEKIDVQTKNQTNKI 616
E + I + I + K+ + N ++ I+ D K+ I+ + ID KN+T K
Sbjct: 307 EGKVKKIASDIKKTKDMLDLNNPDS-SKVIISDIHQKITGIEKSMSHVIDGPEKNKTTKA 365
Query: 617 KSS 625
KSS
Sbjct: 366 KSS 368
>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
HMM hit: AMP-binding enzymes PF00501
Length = 691
Score = 28.3 bits (60), Expect = 6.8
Identities = 11/41 (26%), Positives = 25/41 (60%)
Frame = +2
Query: 470 NRIKENITKGNTGVINEAILLDKPIKVNQIKSGIEEKIDVQ 592
N+IK+ TKG+ + I+ P +++++ G+ +K+D +
Sbjct: 328 NKIKKG-TKGDVTALKPTIMTAVPAILDRVRDGVRKKVDAK 367
>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor [Homo
sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1706
Score = 27.9 bits (59), Expect = 8.9
Identities = 19/53 (35%), Positives = 25/53 (47%)
Frame = -1
Query: 206 IHV*ILESPFQISTS*NMEKIIHTVKGRPLINDE*TTRELCLLLKTAVYLPAA 48
I V ++SPFQ ST + ++H G E RE+ L LK A L A
Sbjct: 1400 IIVGFIKSPFQGSTGSGISVLLHLADGLARSASEDEWREIFLALKEAASLTFA 1452
>At2g04330.1 68415.m00429 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 564
Score = 27.9 bits (59), Expect = 8.9
Identities = 16/58 (27%), Positives = 29/58 (50%)
Frame = +2
Query: 491 TKGNTGVINEAILLDKPIKVNQIKSGIEEKIDVQTKNQTNKIKSSNERDKGKTTTTER 664
T T E+ +LD + +K E + Q QT++++ NE+++GK T T +
Sbjct: 94 TAPTTAPTTESPMLDDSTFYDALKHIPAE--ETQENMQTDEVEDENEKEEGKETETNK 149
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,391,620
Number of Sequences: 28952
Number of extensions: 267412
Number of successful extensions: 771
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 756
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 771
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1950880000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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