Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_L13
         (856 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   266   4e-72
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   258   5e-70
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   132   5e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   118   1e-27
SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual      26   5.9  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.8  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.8  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  266 bits (651), Expect = 4e-72
 Identities = 116/174 (66%), Positives = 141/174 (81%)
 Frame = +1

Query: 115 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 294
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 295 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 474
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 475 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 636
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPA
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174



 Score = 72.9 bits (171), Expect = 5e-14
 Identities = 32/52 (61%), Positives = 36/52 (69%)
 Frame = +3

Query: 630 PRASGXTAVVEPYNSILTTHTTXEHSDCAFMVDNEXIYDICRRXPXXERPXY 785
           P     T+VVEPYNS+LTTH T + +DC FMVDNE  YDICRR    ERP Y
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  258 bits (633), Expect = 5e-70
 Identities = 117/179 (65%), Positives = 143/179 (79%), Gaps = 5/179 (2%)
 Frame = +1

Query: 115 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 279
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 280 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 459
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 460 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 636
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPA
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178



 Score = 75.8 bits (178), Expect = 7e-15
 Identities = 33/52 (63%), Positives = 37/52 (71%)
 Frame = +3

Query: 630 PRASGXTAVVEPYNSILTTHTTXEHSDCAFMVDNEXIYDICRRXPXXERPXY 785
           P     T+VVEPYNS+LTTH T ++SDC FMVDNE  YDICRR    ERP Y
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  132 bits (320), Expect = 5e-32
 Identities = 65/174 (37%), Positives = 94/174 (54%)
 Frame = +1

Query: 115 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 294
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 295 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 474
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 475 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 636
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PA
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172



 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 19/52 (36%), Positives = 25/52 (48%)
 Frame = +3

Query: 630 PRASGXTAVVEPYNSILTTHTTXEHSDCAFMVDNEXIYDICRRXPXXERPXY 785
           P       VVEPYN+ L+ H   E+SD  F +DNE +  I       + P Y
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  118 bits (283), Expect = 1e-27
 Identities = 62/174 (35%), Positives = 98/174 (56%), Gaps = 2/174 (1%)
 Frame = +1

Query: 118 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 297
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 298 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 471
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 472 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 633
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173



 Score = 26.2 bits (55), Expect = 5.9
 Identities = 12/44 (27%), Positives = 21/44 (47%)
 Frame = +3

Query: 654 VVEPYNSILTTHTTXEHSDCAFMVDNEXIYDICRRXPXXERPXY 785
           VV+PYNS+L       ++D   ++DN  +  I       + P +
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTF 225


>SPBC19G7.08c |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 483

 Score = 26.2 bits (55), Expect = 5.9
 Identities = 11/29 (37%), Positives = 14/29 (48%)
 Frame = +3

Query: 624 HLPRASGXTAVVEPYNSILTTHTTXEHSD 710
           HLP ASG +    PY    +  TT   +D
Sbjct: 155 HLPNASGSSVYQPPYTPFRSVSTTSSSND 183


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +2

Query: 194 STASSLMARCPQTRPSGVETILSTLSSARPELAST 298
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +3

Query: 213 WPDAHRQDHRGWRRFFQHFLQR 278
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,217,175
Number of Sequences: 5004
Number of extensions: 66263
Number of successful extensions: 210
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)