Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_K10
(844 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 143 1e-34
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 142 3e-34
At2g47550.1 68415.m05934 pectinesterase family protein contains ... 30 1.7
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 30 1.7
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 30 1.7
At4g22120.1 68417.m03198 early-responsive to dehydration protein... 29 5.1
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein 29 5.1
At1g18670.1 68414.m02330 protein kinase family protein contains ... 29 5.1
At5g14650.1 68418.m01716 polygalacturonase, putative / pectinase... 28 6.8
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 28 6.8
At1g21326.1 68414.m02666 VQ motif-containing protein contains PF... 28 6.8
At4g12470.1 68417.m01972 protease inhibitor/seed storage/lipid t... 28 8.9
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 143 bits (347), Expect = 1e-34
Identities = 69/143 (48%), Positives = 87/143 (60%), Gaps = 1/143 (0%)
Frame = +3
Query: 369 AFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIIEKIPEL 548
AFGNMCRGGRMFAPTK WRRWH ARGH IE +PE+
Sbjct: 97 AFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENVPEM 156
Query: 549 PLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLII 728
PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKGPL++
Sbjct: 157 PLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVV 216
Query: 729 FNKDQG-LTRAFRNIPGVELLNV 794
F + + +AFRN+PGVEL +V
Sbjct: 217 FGTEGAKIVKAFRNLPGVELCHV 239
Score = 111 bits (267), Expect = 6e-25
Identities = 49/63 (77%), Positives = 55/63 (87%)
Frame = +2
Query: 176 VFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHR 355
V AP+RPD+VN VH +S NSRQPY VSK+AGHQTSAESWGTGRAV+RIPRV GGGTHR
Sbjct: 33 VMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHR 92
Query: 356 SGQ 364
+GQ
Sbjct: 93 AGQ 95
Score = 32.7 bits (71), Expect = 0.31
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +2
Query: 797 KLNLLKLAPGGHFGR 841
+LNLLKLAPGGH GR
Sbjct: 241 RLNLLKLAPGGHLGR 255
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 142 bits (344), Expect = 3e-34
Identities = 68/143 (47%), Positives = 87/143 (60%), Gaps = 1/143 (0%)
Frame = +3
Query: 369 AFGNMCRGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXXXXXXXXQARGHIIEKIPEL 548
AFGNMCRGGRMFAPTK WRRWH ARGH IE +PE+
Sbjct: 96 AFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENVPEM 155
Query: 549 PLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKGPLII 728
PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKGPL++
Sbjct: 156 PLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVV 215
Query: 729 FNKD-QGLTRAFRNIPGVELLNV 794
+ + + +AFRN+PGVEL +V
Sbjct: 216 YGTEGSKIVKAFRNLPGVELCHV 238
Score = 111 bits (267), Expect = 6e-25
Identities = 49/63 (77%), Positives = 55/63 (87%)
Frame = +2
Query: 176 VFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHR 355
V AP+RPD+VN VH +S NSRQPY VSK+AGHQTSAESWGTGRAV+RIPRV GGGTHR
Sbjct: 32 VMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHR 91
Query: 356 SGQ 364
+GQ
Sbjct: 92 AGQ 94
Score = 33.1 bits (72), Expect = 0.24
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +2
Query: 734 QGSGSDSRFPQHPRCGAPECXKLNLLKLAPGGHFGR 841
+GS F P +LNLLKLAPGGH GR
Sbjct: 219 EGSKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGR 254
>At2g47550.1 68415.m05934 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 560
Score = 30.3 bits (65), Expect = 1.7
Identities = 24/80 (30%), Positives = 38/80 (47%), Gaps = 2/80 (2%)
Frame = -3
Query: 758 SASQTLILVED--YEGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGL 585
+A T+ +ED + SLT+D + T STKTL L +D+ L A ++
Sbjct: 92 AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQT--C 149
Query: 584 VDLLDFVGYNQGKLGNLFNN 525
++ L G G+LFN+
Sbjct: 150 LEGLKSTASENGLSGDLFND 169
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 30.3 bits (65), Expect = 1.7
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +2
Query: 389 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCCYWRPSARSG*RTHY*KDSRASLGCSRQ 568
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLCP-SPLSQSSKQHHIRLDNHFDTSTPRS 523
Query: 569 S 571
S
Sbjct: 524 S 524
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 30.3 bits (65), Expect = 1.7
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +2
Query: 389 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCCYWRPSARSG*RTHY*KDSRASLGCSRQ 568
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLCP-SPLSQSSKQHHIRLDNHFDTSTPRS 522
Query: 569 S 571
S
Sbjct: 523 S 523
>At4g22120.1 68417.m03198 early-responsive to dehydration
protein-related / ERD protein-related similar to ERD4
protein (early-responsive to dehydration stress)
[Arabidopsis thaliana] GI:15375406; contains Pfam
profile PF02714: Domain of unknown function DUF221
Length = 771
Score = 28.7 bits (61), Expect = 5.1
Identities = 12/42 (28%), Positives = 18/42 (42%)
Frame = -3
Query: 419 GLRGGEHTSTTTHVTEGXPDRTYGYHHHGHAEFGQQHVRYPM 294
GL G +H + P T G+HH + +RYP+
Sbjct: 644 GLLGTKHAALAAPFLIALPVLTIGFHHFCKGRYEPAFIRYPL 685
>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
Length = 320
Score = 28.7 bits (61), Expect = 5.1
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = -3
Query: 722 EGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKL 543
E SL +D A F ++ +K + L+ KD+R ++ ++ D++ G + K+
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301
Query: 542 GNL 534
G +
Sbjct: 302 GEI 304
>At1g18670.1 68414.m02330 protein kinase family protein contains
Protein kinases ATP-binding region signature,
PROSITE:PS00107 and Serine/Threonine protein kinases
active-site signature, PROSITE:PS00108
Length = 662
Score = 28.7 bits (61), Expect = 5.1
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +2
Query: 506 RSG*RTHY*KDSRASLGCSRQSPGDQQDQTGCHLPEAPQGMV*YP*GVQVSASS 667
R+G H DS ++L Q P + + H+ A QG V + +QVS SS
Sbjct: 491 RNGHSVHNSIDSDSTLFEKMQKPSNHEKDEASHVKNASQGDVPFSGPLQVSVSS 544
>At5g14650.1 68418.m01716 polygalacturonase, putative / pectinase,
putative similar to polygalacturonase PG1 GP|5669846,
PG2 GI:5669848 from [Glycine max]; contains PF00295:
Glycosyl hydrolases family 28 (polygalacturonases)
Length = 435
Score = 28.3 bits (60), Expect = 6.8
Identities = 32/121 (26%), Positives = 51/121 (42%), Gaps = 4/121 (3%)
Frame = +2
Query: 281 TSAESWGTGRAVARIPRVRGGGTHRSGQGCLR*HVS-WWTYVRPHEALAALAPSRQPPTA 457
TSA +WG+G + I G G+G + S WW ++ + P R PTA
Sbjct: 121 TSASAWGSG-LLQWIEFKALQGITIKGKGIIDGRGSVWW-----NDMMGTKMP-RTKPTA 173
Query: 458 ESGLGSSRCCYWRPSARSG*RTHY*KDSRASLGCS---RQSPGDQQDQTGCHLPEAPQGM 628
GS+ + ++ +TH D+ S+ S SPGD + G HL + +
Sbjct: 174 LRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQDAV 233
Query: 629 V 631
+
Sbjct: 234 I 234
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 28.3 bits (60), Expect = 6.8
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -1
Query: 595 CLVLLISWTLSATTKGSSGIFS 530
CLVLL+S +A TK SGIF+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFT 30
>At1g21326.1 68414.m02666 VQ motif-containing protein contains
PF05678: VQ motif
Length = 239
Score = 28.3 bits (60), Expect = 6.8
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -3
Query: 374 EGXPDRTYGYHHHGHAEFGQQH 309
EG D+ Y YHHH H + QQH
Sbjct: 144 EGTMDQYYHYHHHHHHQ-EQQH 164
>At4g12470.1 68417.m01972 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to pEARLI
1 (Accession No. L43080): an Arabidopsis member of a
conserved gene family (PGF95-099), Plant Physiol. 109
(4), 1497 (1995); contains Pfam protease inhibitor/seed
storage/LTP family domain PF00234
Length = 161
Score = 27.9 bits (59), Expect = 8.9
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = -3
Query: 548 KLGNLFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVPAPPGLR 411
KLG N +SS LN + SS CC L +VDA + LR
Sbjct: 84 KLGVCANVLSSLLNIQLGQPSSQQCCSLIQGLVDVDAAICLCTALR 129
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,020,061
Number of Sequences: 28952
Number of extensions: 420750
Number of successful extensions: 1275
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1268
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1950880000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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