BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_J24 (851 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 195 6e-51 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 195 6e-51 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 149 7e-37 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 89 7e-19 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 77 2e-15 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 75 1e-14 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 69 8e-13 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 41 2e-04 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.029 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.029 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.029 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 30 0.48 SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 28 1.5 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 28 1.5 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 28 1.9 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 28 1.9 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 27 3.4 SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 27 3.4 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 5.9 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 7.8 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 195 bits (476), Expect = 6e-51 Identities = 99/156 (63%), Positives = 120/156 (76%), Gaps = 1/156 (0%) Frame = +3 Query: 159 MMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIK 338 +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR DEQ+R +TIK Sbjct: 12 LMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIK 71 Query: 339 STAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDGAL 515 STAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDGAL Sbjct: 72 STAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGAL 126 Query: 516 XXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 623 QTETVLRQA+ ERI+P++ +NK+DR Sbjct: 127 VVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDR 162 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 195 bits (476), Expect = 6e-51 Identities = 99/156 (63%), Positives = 120/156 (76%), Gaps = 1/156 (0%) Frame = +3 Query: 159 MMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIK 338 +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR DEQ+R +TIK Sbjct: 12 LMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIK 71 Query: 339 STAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDGAL 515 STAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDGAL Sbjct: 72 STAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGAL 126 Query: 516 XXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 623 QTETVLRQA+ ERI+P++ +NK+DR Sbjct: 127 VVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDR 162 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 149 bits (360), Expect = 7e-37 Identities = 81/155 (52%), Positives = 106/155 (68%) Frame = +3 Query: 159 MMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIK 338 + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R IT+K Sbjct: 12 LQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRGITMK 71 Query: 339 STAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALX 518 S+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ DGA Sbjct: 72 SSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLCDGAFV 123 Query: 519 XXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 623 QT TVLRQA +RIK IL +NKMDR Sbjct: 124 LVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDR 158 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 89.0 bits (211), Expect = 7e-19 Identities = 60/160 (37%), Positives = 84/160 (52%), Gaps = 7/160 (4%) Frame = +3 Query: 165 DKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA--GETRF---TDTRKDEQDR 323 DKKR IRN+ + AH+D GK+T T+ ++ G I G+ D + E+++ Sbjct: 52 DKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAKMDFMELEREK 111 Query: 324 CITIKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVT 503 ITI+S A +E + N Q+ EK + IN+ID+PGH+DF+ EV ALRV Sbjct: 112 GITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVL 169 Query: 504 DGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 623 DGA+ QT TV RQ + I F+NKMDR Sbjct: 170 DGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDR 209 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 77.4 bits (182), Expect = 2e-15 Identities = 55/151 (36%), Positives = 78/151 (51%), Gaps = 2/151 (1%) Frame = +3 Query: 177 NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFT--DTRKDEQDRCITIKSTAI 350 +IRN+ +IAH+D GK+TLT+ ++ G + +T T D E+ R ITI S AI Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAI 86 Query: 351 SMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXX 530 S + +QR INLID+PGH DF+ EV ++ V DGA+ Sbjct: 87 SFTWR------------NQR--------INLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126 Query: 531 XXXXXXQTETVLRQAIAERIKPILFMNKMDR 623 QT+ V +QA I ++F+NKMDR Sbjct: 127 SAGVEAQTKVVWKQATKRGIPKVIFVNKMDR 157 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 75.4 bits (177), Expect = 1e-14 Identities = 47/150 (31%), Positives = 75/150 (50%) Frame = +3 Query: 171 KRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAI 350 + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R IT+K+ Sbjct: 55 QNRVRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGITVKAQTC 113 Query: 351 SMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXX 530 SM + + +L+NLID+PGHVDF +EV +L +G + Sbjct: 114 SMIYYYH----------------GQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDA 157 Query: 531 XXXXXXQTETVLRQAIAERIKPILFMNKMD 620 QT + A ++ + I +NK+D Sbjct: 158 SQGIQAQTLSNFYMAFSQNLVIIPVLNKVD 187 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 68.9 bits (161), Expect = 8e-13 Identities = 46/157 (29%), Positives = 75/157 (47%), Gaps = 1/157 (0%) Frame = +3 Query: 156 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 332 G++ ++R+ V H+ HGKS L D LV + R+TDT E++R ++ Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191 Query: 333 IKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 512 IKST +++ D + K+ F ID+PGHVDF EV A + ++DG Sbjct: 192 IKSTPLTLAVS------------DMKGKT---FAFQCIDTPGHVDFVDEVAAPMAISDGV 236 Query: 513 LXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 623 + T +++ AI + +L +NK+DR Sbjct: 237 VLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKVDR 273 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 41.1 bits (92), Expect = 2e-04 Identities = 39/152 (25%), Positives = 61/152 (40%), Gaps = 1/152 (0%) Frame = +3 Query: 168 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTA 347 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R ITI S Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAH 108 Query: 348 ISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXX 527 + +E + +D PGH D+ + DGA+ Sbjct: 109 VE--YETANRHYAH------------------VDCPGHADYIKNMITGAATMDGAIIVVS 148 Query: 528 XXXXXXXQTETVLRQAIAERIKPI-LFMNKMD 620 QT L A +K I +++NK+D Sbjct: 149 ATDGQMPQTREHLLLARQVGVKQIVVYINKVD 180 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.029 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 162 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 257 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.029 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 162 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 257 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.029 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 162 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 257 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 29.9 bits (64), Expect = 0.48 Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 4/67 (5%) Frame = +3 Query: 189 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR----FTDTRKDEQDRCITIKSTAISM 356 ++++ HVDHGK+TL D+ K+ I + G T+ FT D+ + IT T M Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQKIGAFT-VPFDKGSKFITFLDTPGHM 231 Query: 357 FFELEEK 377 FE K Sbjct: 232 AFEAMRK 238 >SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 28.3 bits (60), Expect = 1.5 Identities = 22/90 (24%), Positives = 43/90 (47%) Frame = +3 Query: 219 KSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITN 398 K+ +T L S+ ++A A R+ D + ++ I +ST +S F D++FI Sbjct: 462 KAGITTILNSRTSVLAAANPIFGRYDDMKTPGEN--IDFQSTILSRF------DMIFIVK 513 Query: 399 PDQREKSEKGFLINLIDSPGHVDFSSEVTA 488 + E ++ ++I+ ++ SSE A Sbjct: 514 DEHDETKDRNIARHVINLHTNLQESSETLA 543 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 28.3 bits (60), Expect = 1.5 Identities = 29/111 (26%), Positives = 47/111 (42%), Gaps = 1/111 (0%) Frame = +3 Query: 186 NMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFE 365 N+ I HVD GKSTL +++ G++ R E + E + S A+ E Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV-DKRTMEK--IEREAKEAGKESWYLSWALDSTSE 296 Query: 366 LEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDF-SSEVTAALRVTDGAL 515 EK E + F +L+D+PGH + ++ + A + G L Sbjct: 297 EREKGKTVEVGRAYFETEHRRF--SLLDAPGHKGYVTNMINGASQADIGVL 345 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 27.9 bits (59), Expect = 1.9 Identities = 19/75 (25%), Positives = 38/75 (50%), Gaps = 1/75 (1%) Frame = -2 Query: 562 TVSVCTHTPD-TQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVMN 386 T + C+ P+ T ST + +V+ S + ++ ST P ++ + S S V + Sbjct: 575 TTTTCSSRPEETISTVSTTSTVSESGSSSASITSTYPSSTLSMTTSHLS-----SSSVHS 629 Query: 385 TKSFSSSSKNIEMAV 341 + + SSSS++ M++ Sbjct: 630 SSAHSSSSRSSSMSL 644 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 27.9 bits (59), Expect = 1.9 Identities = 21/76 (27%), Positives = 36/76 (47%), Gaps = 1/76 (1%) Frame = -2 Query: 565 STVSVCTHTPDTQSTT-TRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 389 +T S + +P + STT T +PS + S++ +S S+ S S S S Sbjct: 135 TTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 194 Query: 388 NTKSFSSSSKNIEMAV 341 ++ S SSSS + + + Sbjct: 195 SSSSSSSSSSSSSVPI 210 Score = 27.1 bits (57), Expect = 3.4 Identities = 19/71 (26%), Positives = 33/71 (46%) Frame = -2 Query: 568 RSTVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 389 ++TVS + + T S+++ +PS + + TS S+ S S S S Sbjct: 124 QTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSS 183 Query: 388 NTKSFSSSSKN 356 ++ S SSSS + Sbjct: 184 SSSSSSSSSSS 194 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 27.1 bits (57), Expect = 3.4 Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Frame = -2 Query: 475 EEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 299 EE + P + K KK S F+ T +S S++NI+ A+DL+ + S S ++ Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125 >SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 971 Score = 27.1 bits (57), Expect = 3.4 Identities = 17/45 (37%), Positives = 22/45 (48%) Frame = +1 Query: 523 LTVCLVCVYKLKQYCVRLLPSASSLFCS*TKWTVLFLSSNLKLXN 657 L V + YKL+ RL +A S+ C WT LF SN+ N Sbjct: 348 LNVIGIAAYKLEDPVHRLFVTAFSVCCECLAWTSLF--SNISPEN 390 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 26.2 bits (55), Expect = 5.9 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = +1 Query: 625 LFLSSNLKLXNYTRRPAYCRKCXRHYSPYNDDGGP 729 +F N+KL NYT P+ +K PY D P Sbjct: 495 IFGVDNIKLINYTSSPSGSKKFVMWNPPYVDPKHP 529 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.8 bits (54), Expect = 7.8 Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = -2 Query: 427 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLMVMQR 320 P S +S W+ V+NTK SFS ++M +L+ Q+ Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,210,298 Number of Sequences: 5004 Number of extensions: 61912 Number of successful extensions: 211 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 192 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 207 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 422462090 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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