BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_J07 (840 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 323 2e-89 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 320 1e-88 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 158 1e-39 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 129 4e-31 SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 7.6 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.6 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.6 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 323 bits (794), Expect = 2e-89 Identities = 145/224 (64%), Positives = 174/224 (77%) Frame = +1 Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465 +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645 +IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+ Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180 Query: 646 VVXPXQLYPHHPHNLEHSDCAFMVDNEXIYDICRRNLXXERPTY 777 VV P L+ +DC FMVDNE YDICRRNL ERP+Y Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 320 bits (787), Expect = 1e-88 Identities = 148/229 (64%), Positives = 177/229 (77%), Gaps = 5/229 (2%) Frame = +1 Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450 TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++ Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119 Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 630 D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179 Query: 631 QVSTAVVXPXQLYPHHPHNLEHSDCAFMVDNEXIYDICRRNLXXERPTY 777 QVST+VV P L++SDC FMVDNE YDICRRNL ERPTY Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 158 bits (383), Expect = 1e-39 Identities = 81/224 (36%), Positives = 117/224 (52%) Frame = +1 Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285 MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465 +VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118 Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 645 +R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178 Query: 646 VVXPXQLYPHHPHNLEHSDCAFMVDNEXIYDICRRNLXXERPTY 777 VV P +E+SD F +DNE + I L + P+Y Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 129 bits (312), Expect = 4e-31 Identities = 75/226 (33%), Positives = 118/226 (52%), Gaps = 3/226 (1%) Frame = +1 Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60 Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462 +PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++ Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119 Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 639 D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179 Query: 640 TAVVXPXQLYPHHPHNLEHSDCAFMVDNEXIYDICRRNLXXERPTY 777 VV P ++D ++DN + I L + PT+ Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTF 225 >SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 25.8 bits (54), Expect = 7.6 Identities = 13/28 (46%), Positives = 16/28 (57%) Frame = +2 Query: 731 SMTSAAVISTLSAQPTQPESSHRTXXSS 814 S +SA + S+ SA PT SSH SS Sbjct: 100 SSSSATLTSSSSASPTSSSSSHALSSSS 127 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.8 bits (54), Expect = 7.6 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = +2 Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289 ST SSL + ++PS T ST SSA P S+ Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 7.6 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +3 Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,187,733 Number of Sequences: 5004 Number of extensions: 64389 Number of successful extensions: 178 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 170 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 414453330 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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