Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_I21
(778 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 248 7e-67
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 241 1e-64
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 133 2e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 113 2e-26
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 28 1.3
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 248 bits (607), Expect = 7e-67
Identities = 107/148 (72%), Positives = 125/148 (84%)
Frame = +3
Query: 102 MRECISVHVGLAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 281
MRE IS+HVG AG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 282 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 461
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 462 RIRKLADQCTGLQGFLIFHSFGGGTALG 545
+IR++AD C+GLQGFL+FHSFGGGT G
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSG 148
Score = 74.9 bits (176), Expect = 1e-14
Identities = 40/78 (51%), Positives = 52/78 (66%), Gaps = 1/78 (1%)
Frame = +2
Query: 536 GSGFTSLLMERLSVDYGKKSKLEFAIYPXPQVSTAVVEPXXSILTXTXP*AL*LC-FXGR 712
GSGF +LL+ERL+++Y KKSKL+F++YP PQVST+VVEP S+LT L C F
Sbjct: 146 GSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVD 205
Query: 713 Q*XXFXICRRYLXXXRPT 766
+ ICRR L RP+
Sbjct: 206 NESCYDICRRNLDIERPS 223
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 241 bits (589), Expect = 1e-64
Identities = 119/212 (56%), Positives = 141/212 (66%), Gaps = 6/212 (2%)
Frame = +3
Query: 102 MRECISVHVGLAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 266
MRE ISVHVG AGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 267 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 446
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 447 DLVLDRIRKLADQCTGLQGFLIFHSFGGGTALGSLPY*WSVSPLTTARSLNWXXXXXXXX 626
D VL+RIR++AD C+GLQGFL+FHSFGGGT G + + N
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 627 XXXXXXXXXXXXXXXPH-XLEPSDCAFXVDNE 719
H L+ SDC F VDNE
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNE 211
Score = 72.5 bits (170), Expect = 6e-14
Identities = 39/78 (50%), Positives = 50/78 (64%), Gaps = 1/78 (1%)
Frame = +2
Query: 536 GSGFTSLLMERLSVDYGKKSKLEFAIYPXPQVSTAVVEPXXSILTXTXP*AL*LC-FXGR 712
GSG +LL+ERL+++YGKKS L+F++YP PQVST+VVEP S+LT C F
Sbjct: 150 GSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVD 209
Query: 713 Q*XXFXICRRYLXXXRPT 766
+ ICRR L RPT
Sbjct: 210 NEACYDICRRNLDIERPT 227
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 133 bits (322), Expect = 2e-32
Identities = 64/148 (43%), Positives = 86/148 (58%)
Frame = +3
Query: 102 MRECISVHVGLAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 281
MRE + + G G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 282 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 461
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 462 RIRKLADQCTGLQGFLIFHSFGGGTALG 545
+R+ A+ C LQGF + HS GGGT G
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSG 146
Score = 34.7 bits (76), Expect = 0.015
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +2
Query: 536 GSGFTSLLMERLSVDYGKKSKLEFAIYPXPQVSTAVVEPXXSILT 670
GSG +LL+ ++ +Y + F++ P P+ S VVEP + L+
Sbjct: 144 GSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 113 bits (273), Expect = 2e-26
Identities = 58/149 (38%), Positives = 88/149 (59%), Gaps = 2/149 (1%)
Frame = +3
Query: 105 RECISVHVGLAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 284
RE I++ G G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 285 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 458
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 459 DRIRKLADQCTGLQGFLIFHSFGGGTALG 545
D I + AD L+GF + HS GGT G
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSG 148
Score = 37.1 bits (82), Expect = 0.003
Identities = 20/45 (44%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = +2
Query: 536 GSGFTSLLMERLSVDYGKKSKLEFAIYPXPQ-VSTAVVEPXXSIL 667
GSG S L+ERL+ Y KK ++++P Q VS VV+P S+L
Sbjct: 146 GSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLL 190
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 28.3 bits (60), Expect = 1.3
Identities = 14/46 (30%), Positives = 26/46 (56%)
Frame = -3
Query: 590 SCRSQRRDAPSIRK*TQSRTSTEGVEDQESLQTGTLVSELADSVQN 453
S + SIR ++SR+S+ G + Q S+Q+GT ++D ++
Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 181 STASSLMARCPQTRPSGVETILSTLSSARPELAST 285
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 200 WPDAHRQDHRGWRRFFQHFLQR 265
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,052,029
Number of Sequences: 5004
Number of extensions: 63254
Number of successful extensions: 169
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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