BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_I16 (840 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 281 9e-77 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 159 3e-40 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 155 1e-38 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 128 1e-30 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 84 3e-17 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 81 1e-16 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 73 7e-14 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 54 3e-08 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.12 SPBPB2B2.06c |||phosphoprotein phosphatase |Schizosaccharomyces ... 28 1.9 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 5.8 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 281 bits (689), Expect = 9e-77 Identities = 134/157 (85%), Positives = 138/157 (87%) Frame = +2 Query: 236 PAIRXVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 415 P +MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97 Query: 416 PEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGD 595 PEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQAVLSLYASGRTTGIVLDSGD Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157 Query: 596 GVSHTXPIYEGYALPHAHPASWT*PXRDLTDYLMXIL 706 GV+HT PIYEGYALPHA RDLTDYLM IL Sbjct: 158 GVTHTVPIYEGYALPHA-IMRLDLAGRDLTDYLMKIL 193 Score = 60.9 bits (141), Expect = 2e-10 Identities = 26/35 (74%), Positives = 31/35 (88%) Frame = +3 Query: 129 KKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 233 +++ LV+DNGSGMCKAGFAGDDAPRAVFPS G+ Sbjct: 3 EEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGR 37 Score = 35.9 bits (79), Expect = 0.007 Identities = 17/27 (62%), Positives = 18/27 (66%) Frame = +1 Query: 754 VRDXKEXLCYVALXFXQEMAXXXSSSS 834 VRD KE LCYVAL F QE+ SSS Sbjct: 209 VRDIKEKLCYVALDFEQELQTAAQSSS 235 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 159 bits (387), Expect = 3e-40 Identities = 76/156 (48%), Positives = 107/156 (68%), Gaps = 1/156 (0%) Frame = +2 Query: 251 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEH 427 VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEH Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106 Query: 428 PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 607 P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166 Query: 608 TXPIYEGYALPHAHPASWT*PXRDLTDYLMXILTRA 715 + PIY+G+++P A RD+TDYL L ++ Sbjct: 167 SVPIYDGFSIPSAIQQEEI-GGRDVTDYLQLQLRKS 201 Score = 44.0 bits (99), Expect = 3e-05 Identities = 17/30 (56%), Positives = 23/30 (76%) Frame = +3 Query: 144 LVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 233 + +DNGSG KAGFAGDD P+ +FP+ G+ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGR 41 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 155 bits (375), Expect = 1e-38 Identities = 71/147 (48%), Positives = 102/147 (69%) Frame = +2 Query: 272 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 451 KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109 Query: 452 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTXPIYEGY 631 +NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H P+YE Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169 Query: 632 ALPHAHPASWT*PXRDLTDYLMXILTR 712 L H RD T YL+ +L R Sbjct: 170 VLNHL-VGRLDVAGRDATRYLISLLLR 195 Score = 35.9 bits (79), Expect = 0.007 Identities = 15/30 (50%), Positives = 21/30 (70%) Frame = +3 Query: 144 LVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 233 +V+DNG+G K G+A D+ PR FPS G+ Sbjct: 6 IVLDNGTGFVKVGYAKDNFPRFQFPSIVGR 35 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 128 bits (308), Expect = 1e-30 Identities = 61/130 (46%), Positives = 82/130 (63%), Gaps = 9/130 (6%) Frame = +2 Query: 275 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 451 D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132 Query: 452 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSH 607 LNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192 Query: 608 TXPIYEGYAL 637 P+ EGY + Sbjct: 193 IIPVAEGYVI 202 Score = 38.3 bits (85), Expect = 0.001 Identities = 14/26 (53%), Positives = 21/26 (80%) Frame = +3 Query: 144 LVVDNGSGMCKAGFAGDDAPRAVFPS 221 +++DNG+G K G+AG+DAP VFP+ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPT 33 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 83.8 bits (198), Expect = 3e-17 Identities = 40/107 (37%), Positives = 59/107 (55%) Frame = +2 Query: 317 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 496 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 497 ETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTXPIYEGYAL 637 E+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169 Score = 33.1 bits (72), Expect = 0.050 Identities = 13/30 (43%), Positives = 20/30 (66%) Frame = +3 Query: 144 LVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 233 +V+D GS + GF+G+D P+ V PS G+ Sbjct: 14 IVIDPGSKWTRIGFSGEDIPKCVLPSYCGE 43 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 81.4 bits (192), Expect = 1e-16 Identities = 43/137 (31%), Positives = 76/137 (55%), Gaps = 4/137 (2%) Frame = +2 Query: 263 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 439 + + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ + Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120 Query: 440 TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTX 613 TE NP R MT+++FE +N P++ I + S Y + + ++GIVL+ G+ SH Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180 Query: 614 PIYEG-YALPHAHPASW 661 P+ G L A SW Sbjct: 181 PVLNGERILSEAKRISW 197 Score = 30.7 bits (66), Expect = 0.27 Identities = 12/24 (50%), Positives = 17/24 (70%) Frame = +3 Query: 144 LVVDNGSGMCKAGFAGDDAPRAVF 215 LV+DNGS +AG+ G+ P+ VF Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVF 50 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 72.5 bits (170), Expect = 7e-14 Identities = 36/133 (27%), Positives = 70/133 (52%), Gaps = 2/133 (1%) Frame = +2 Query: 236 PAIRXVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 409 P+ V + ++ YV DE Q I + +K +GI+ +W+ W +L+ Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96 Query: 410 VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDS 589 V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDI 156 Query: 590 GDGVSHTXPIYEG 628 G + P+ +G Sbjct: 157 GSDNASVTPVVDG 169 Score = 34.7 bits (76), Expect = 0.017 Identities = 13/34 (38%), Positives = 23/34 (67%) Frame = +3 Query: 129 KKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSNRG 230 +++P LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 54.0 bits (124), Expect = 3e-08 Identities = 32/103 (31%), Positives = 49/103 (47%), Gaps = 4/103 (3%) Frame = +2 Query: 332 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 499 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 500 TFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTXPIYEG 628 P +A + ++ LYA G G+V+D G + PI +G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.12 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +2 Query: 392 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 535 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPBPB2B2.06c |||phosphoprotein phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 601 Score = 27.9 bits (59), Expect = 1.9 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = +3 Query: 108 TNSRRATKKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSN 224 TN R+T +PR+++ NG G+ + + G P +F N Sbjct: 418 TNRNRST--VPRIIISNGGGIRGSMYQGKFGPDEMFQLN 454 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 5.8 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 434 LLTEAPLNPKANREKMTQIMFETFN 508 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,072,808 Number of Sequences: 5004 Number of extensions: 60213 Number of successful extensions: 164 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 414453330 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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