Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_I15
(817 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 34 0.13
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 32 0.40
At1g16840.2 68414.m02028 expressed protein 32 0.52
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 31 1.2
At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 30 1.6
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 30 2.1
At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 30 2.1
At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 30 2.1
At4g02520.1 68417.m00345 glutathione S-transferase, putative 29 2.8
At2g02930.1 68415.m00241 glutathione S-transferase, putative 29 2.8
At3g49210.1 68416.m05378 expressed protein 29 3.7
At2g06200.1 68415.m00682 expressed protein 29 4.9
At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 28 8.5
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 28 8.5
>At1g02930.1 68414.m00260 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 208
Score = 33.9 bits (74), Expect = 0.13
Identities = 19/41 (46%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
Frame = +1
Query: 25 GKVPAFESADGKVLLTESNAIAYYVANE-SLRGGDLATQAR 144
GKVPAFE D K+ ES AI Y+A+E S +G +L + +
Sbjct: 53 GKVPAFEDGDFKIF--ESRAITQYIAHEFSDKGNNLLSTGK 91
>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
domain-containing protein-related contains weak hit to
Pfam F00627: UBA/TS-N domain; supported by tandem
duplication of ubiquitin-associated (UBA)/TS-N domain
protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
thaliana]
Length = 508
Score = 32.3 bits (70), Expect = 0.40
Identities = 21/95 (22%), Positives = 37/95 (38%), Gaps = 1/95 (1%)
Frame = +2
Query: 215 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLVTER-ITLADVIVFXYTAACFPARA 391
L++ + N N +S + WPP K W D H + E + + ++ A R+
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSREEAFYYLEGSKWNFSRAVEACRS 254
Query: 392 RPERPFVADKRSALVPDRRPPAASVGRRRLAHALC 496
+ A + SA P P S+ + + C
Sbjct: 255 KTLPVVSASQESAEAPPNSPEQPSLLKNEWISSFC 289
>At1g16840.2 68414.m02028 expressed protein
Length = 161
Score = 31.9 bits (69), Expect = 0.52
Identities = 26/80 (32%), Positives = 34/80 (42%)
Frame = +2
Query: 53 MERCF*LKAMPSLTTLPMKVSAEEIWLPKPVSGSGHHGLTANYCLLPALGSSLTLVSCNS 232
+ R F +A P LP V W+P P SGS HG+ L+ L + LT S
Sbjct: 67 VHRIFVRRAQPDW--LPF-VPGASYWVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLS 120
Query: 233 TNRMLNVQSLTYWPP*KYWT 292
TN S Y+ YW+
Sbjct: 121 TNSSHGWPSSDYFLKGSYWS 140
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 30.7 bits (66), Expect = 1.2
Identities = 16/49 (32%), Positives = 24/49 (48%)
Frame = +3
Query: 378 FQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTYDPKK 524
F V+ S +V+R+F TV +QP + V+G + A P KK
Sbjct: 171 FATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTEAVPPIASKK 219
>At1g02920.1 68414.m00259 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]; supported by cDNA GI:443697.
Length = 209
Score = 30.3 bits (65), Expect = 1.6
Identities = 16/27 (59%), Positives = 18/27 (66%)
Frame = +1
Query: 25 GKVPAFESADGKVLLTESNAIAYYVAN 105
GKVPAFE DG L ES AI Y+A+
Sbjct: 53 GKVPAFE--DGDFKLFESRAITQYIAH 77
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 29.9 bits (64), Expect = 2.1
Identities = 15/49 (30%), Positives = 24/49 (48%)
Frame = +3
Query: 378 FQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTYDPKK 524
F V+ S+ +V+R+F T+ +QP+ V+G A P KK
Sbjct: 171 FATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPVPTKK 219
>At1g02950.2 68414.m00263 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 245
Score = 29.9 bits (64), Expect = 2.1
Identities = 19/36 (52%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +1
Query: 25 GKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 129
G+VP FE DG V L ES AI Y+A S RG L
Sbjct: 76 GQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 109
>At1g02950.1 68414.m00262 glutathione S-transferase, putative
similar to glutathione-S-transferase GI:169887 from
[Silene vulgaris]
Length = 243
Score = 29.9 bits (64), Expect = 2.1
Identities = 19/36 (52%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +1
Query: 25 GKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 129
G+VP FE DG V L ES AI Y+A S RG L
Sbjct: 74 GQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 107
>At4g02520.1 68417.m00345 glutathione S-transferase, putative
Length = 212
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/27 (55%), Positives = 19/27 (70%)
Frame = +1
Query: 25 GKVPAFESADGKVLLTESNAIAYYVAN 105
G+VPAFE DG + L ES AI Y+A+
Sbjct: 53 GQVPAFE--DGDLKLFESRAITQYIAH 77
>At2g02930.1 68415.m00241 glutathione S-transferase, putative
Length = 212
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/27 (55%), Positives = 19/27 (70%)
Frame = +1
Query: 25 GKVPAFESADGKVLLTESNAIAYYVAN 105
G+VPAFE DG + L ES AI Y+A+
Sbjct: 53 GQVPAFE--DGDLKLFESRAITQYIAH 77
>At3g49210.1 68416.m05378 expressed protein
Length = 518
Score = 29.1 bits (62), Expect = 3.7
Identities = 16/64 (25%), Positives = 30/64 (46%)
Frame = +1
Query: 124 DLATQARVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLL 303
D+ + +W +W + P S L ++ K+ ++R K+ L AAL + G +
Sbjct: 352 DMMAKGSTCRWGNWIGYIVFPFSIGLRDDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFI 411
Query: 304 TRTF 315
+TF
Sbjct: 412 LKTF 415
>At2g06200.1 68415.m00682 expressed protein
Length = 244
Score = 28.7 bits (61), Expect = 4.9
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = -1
Query: 439 RNQR*TFISDERTLGSSTCWKACS-SVXEDNDIGKCDSLGNQ 317
R +R ++ D ++ S K+CS S EDND G C + N+
Sbjct: 139 RRRRSGYMDDFFSIEPSGSIKSCSGSAMEDNDDGSCRGINNE 180
>At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like
transposon protein GB:AAB95292 GI:2088658 from
[Arabidopsis thaliana]
Length = 1148
Score = 27.9 bits (59), Expect = 8.5
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -2
Query: 483 ASRRRPTLAAGGRRSGTNAERLSATNGR 400
+S R P+ A GRRSGT R+S T G+
Sbjct: 171 SSARHPS-PASGRRSGTPVRRISPTPGK 197
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 27.9 bits (59), Expect = 8.5
Identities = 18/34 (52%), Positives = 22/34 (64%), Gaps = 4/34 (11%)
Frame = +1
Query: 13 SFLA----GKVPAFESADGKVLLTESNAIAYYVA 102
SFLA G+VP F DG + LTES AI+ Y+A
Sbjct: 80 SFLAINPFGQVPVF--LDGGLKLTESRAISEYIA 111
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,594,315
Number of Sequences: 28952
Number of extensions: 310635
Number of successful extensions: 814
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 801
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 814
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1863090400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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