BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_I10 (822 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 270 1e-73 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 263 2e-71 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 134 1e-32 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 117 2e-27 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.4 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.4 SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.8 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 270 bits (663), Expect = 1e-73 Identities = 119/177 (67%), Positives = 143/177 (80%) Frame = +1 Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285 MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465 +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLKERLSVDYGKKSKLEFAIYPAPQV 636 +IR++AD C+GLQGFL+FH LL ERL+++Y KKSKL+F++YPAPQV Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177 Score = 48.4 bits (110), Expect = 1e-06 Identities = 20/27 (74%), Positives = 23/27 (85%) Frame = +2 Query: 686 LEHSDCAFMVDNEAIYDICRRXLDIER 766 L+ +DC FMVDNE+ YDICRR LDIER Sbjct: 195 LDLADCTFMVDNESCYDICRRNLDIER 221 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 263 bits (645), Expect = 2e-71 Identities = 120/182 (65%), Positives = 145/182 (79%), Gaps = 5/182 (2%) Frame = +1 Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270 MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59 Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450 TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++ Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119 Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLKERLSVDYGKKSKLEFAIYPAP 630 D VL+RIR++AD C+GLQGFL+FH LL ERL+++YGKKS L+F++YPAP Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179 Query: 631 QV 636 QV Sbjct: 180 QV 181 Score = 52.8 bits (121), Expect = 6e-08 Identities = 24/30 (80%), Positives = 26/30 (86%) Frame = +2 Query: 686 LEHSDCAFMVDNEAIYDICRRXLDIERXPT 775 L++SDC FMVDNEA YDICRR LDIER PT Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIER-PT 227 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 134 bits (324), Expect = 1e-32 Identities = 66/176 (37%), Positives = 95/176 (53%) Frame = +1 Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285 MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58 Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465 +VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118 Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLKERLSVDYGKKSKLEFAIYPAPQ 633 +R+ A+ C LQGF + H LL ++ +Y + F++ PAP+ Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 117 bits (281), Expect = 2e-27 Identities = 63/177 (35%), Positives = 98/177 (55%), Gaps = 2/177 (1%) Frame = +1 Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288 RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60 Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462 +PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++ Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119 Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLKERLSVDYGKKSKLEFAIYPAPQ 633 D I + AD L+GF + H L ERL+ Y KK ++++P Q Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.8 bits (54), Expect = 7.4 Identities = 15/35 (42%), Positives = 19/35 (54%) Frame = +2 Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELAST 289 ST SSL + ++PS T ST SSA P S+ Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 7.4 Identities = 13/22 (59%), Positives = 13/22 (59%) Frame = +3 Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269 W A R D R R FQHFLQR Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611 >SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pombe|chr 3|||Manual Length = 534 Score = 25.4 bits (53), Expect = 9.8 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +2 Query: 95 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 217 L+S +L+ P SR++ P S + STASSL P Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,262,677 Number of Sequences: 5004 Number of extensions: 68085 Number of successful extensions: 242 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 238 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 402440190 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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