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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_I06
         (858 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit...    49   1e-06
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po...    37   0.004
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta...    30   0.48 
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    27   3.4  
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch...    27   3.4  
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ...    27   3.4  
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces...    27   4.5  
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ...    26   6.0  
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo...    26   6.0  
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c...    26   7.9  

>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 48.8 bits (111), Expect = 1e-06
 Identities = 34/114 (29%), Positives = 59/114 (51%), Gaps = 4/114 (3%)
 Frame = +2

Query: 146 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 313
           N   D   KFP  K+P F   DG   L+E+ AIA+Y+A+     +L G     +A+V Q+
Sbjct: 38  NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96

Query: 314 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 475
            S+++SEL  A    + P +    +++Q  + A++ +       D  L ++T+L
Sbjct: 97  CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYL 150



 Score = 35.1 bits (77), Expect = 0.013
 Identities = 21/77 (27%), Positives = 36/77 (46%)
 Frame = +3

Query: 474 LVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCA 653
           LV  R+TLAD+     L     +VL  S  +   ++ R++ T+ HQ ++ A+   L    
Sbjct: 150 LVGSRLTLADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAITEPLKFID 209

Query: 654 APPTYDPKKYQELAGAQ 704
            P      + +E A A+
Sbjct: 210 QPLPIIKAENKEAAPAK 226


>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 220

 Score = 36.7 bits (81), Expect = 0.004
 Identities = 17/49 (34%), Positives = 29/49 (59%)
 Frame = +3

Query: 474 LVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQV 620
           LV +R TLAD+   S L   F  ++D   R  L ++ R+++T+ HQ ++
Sbjct: 155 LVGDRFTLADLFFGSLLRIFFNSIIDEKTRKELPHLTRYYITMFHQAKL 203



 Score = 35.5 bits (78), Expect = 0.010
 Identities = 35/158 (22%), Positives = 67/158 (42%), Gaps = 8/158 (5%)
 Frame = +2

Query: 26  MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 205
           M  G LY++  N R    L  A+     V +   +       S D   KFP  K+P F  
Sbjct: 1   MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57

Query: 206 ADGKVLLTESNAIAYYVA------NESLRGGDLATQARVWQWASWSDSELL-PASC-AWV 361
           ADG  L      + Y+         E L   +   +A + +W  + + +++ P +   WV
Sbjct: 58  ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117

Query: 362 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 475
             + G + + ++  + + +  + +LK+ +  +  RT+L
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYL 155


>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 450

 Score = 29.9 bits (64), Expect = 0.48
 Identities = 19/66 (28%), Positives = 32/66 (48%)
 Frame = +3

Query: 489 ITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTY 668
           I++AD+ V++ + H++   L       L NV RWF  + HQ  V     S+++  A    
Sbjct: 144 ISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESVMEAANSMSMKLANIDL 202

Query: 669 DPKKYQ 686
           +  K Q
Sbjct: 203 NAPKIQ 208


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 11/37 (29%), Positives = 21/37 (56%)
 Frame = -3

Query: 547 STCWKACSSVLKTMTSASVILSVTKEGACEKMSVQYF 437
           ST W A ++ ++   + + I     + AC++ S+QYF
Sbjct: 317 STAWYAMANRMRGKLNVAHINCAVSKRACKQYSIQYF 353


>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
           Eme1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 16/62 (25%), Positives = 30/62 (48%)
 Frame = +2

Query: 53  PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 232
           P  F++ KA +  QY+          +FG  +K ++F K+    ++  F S +  +L+  
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611

Query: 233 SN 238
           SN
Sbjct: 612 SN 613


>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 267

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
 Frame = +3

Query: 282 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 377
           +W+P PV  SG    T   C+ LP  G  +T V
Sbjct: 23  LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55


>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 442

 Score = 26.6 bits (56), Expect = 4.5
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = +3

Query: 420 TYWPP*KYWTDIFSHAPSLVT 482
           TY PP  Y  D+FS APS+ +
Sbjct: 257 TYLPPFNYDHDVFSFAPSVAS 277


>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 369

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -1

Query: 222 STFPSALSNAGTFPAGNFFKKSSDL 148
           +TFP A +  GTFP G  F   S L
Sbjct: 52  ATFPWAQAPVGTFPIGQLFDPVSGL 76


>SPBC16A3.11 |eso1||sister chromatid cohesion protein
           Eso1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 872

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = +2

Query: 233 SNAIAYYVANESLRGGDLATQARVWQWAS 319
           S + A Y+ NE+LR G +   +R+W  AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808


>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 339

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
 Frame = +3

Query: 312 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 419
           GH  L  + C  P++GSS  L+  +C   N R+  V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,475,433
Number of Sequences: 5004
Number of extensions: 72078
Number of successful extensions: 230
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 222
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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