BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_I06 (858 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 107 1e-23 At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 104 7e-23 At1g02930.1 68414.m00260 glutathione S-transferase, putative sim... 46 2e-05 At4g02520.1 68417.m00345 glutathione S-transferase, putative 45 7e-05 At2g02930.1 68415.m00241 glutathione S-transferase, putative 44 1e-04 At1g02920.1 68414.m00259 glutathione S-transferase, putative sim... 42 5e-04 At2g02380.1 68415.m00176 glutathione S-transferase, putative sim... 40 0.003 At1g02950.2 68414.m00263 glutathione S-transferase, putative sim... 38 0.006 At1g02950.1 68414.m00262 glutathione S-transferase, putative sim... 38 0.006 At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 38 0.006 At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 38 0.009 At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 38 0.009 At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 35 0.080 At1g16840.2 68414.m02028 expressed protein 32 0.56 At5g46550.1 68418.m05731 DNA-binding bromodomain-containing prot... 29 3.0 At3g14270.1 68416.m01806 phosphatidylinositol-4-phosphate 5-kina... 29 3.0 At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-... 29 3.0 At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 29 3.0 At3g49210.1 68416.m05378 expressed protein 29 4.0 At4g22130.1 68417.m03199 protein kinase family protein contains ... 29 5.2 At5g20930.1 68418.m02486 protein kinase, putative nearly identic... 28 9.1 At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 28 9.1 >At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / eEF-1B gamma, putative Similar to elongation factor 1-gamma (gb|EF1G_XENLA). ESTs gb|T20564,gb|T45940,gb|T04527 come from this gene Length = 414 Score = 107 bits (256), Expect = 1e-23 Identities = 63/150 (42%), Positives = 83/150 (55%), Gaps = 4/150 (2%) Frame = +2 Query: 38 VLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGK 217 VL+TY N A KALIAA+Y G + V +F G TNK+ FLK P GKVP E+ +G Sbjct: 4 VLHTYKGNKSAEKALIAAEYVGVQIDVPSDFQMGVTNKTPAFLKMNPIGKVPVLETPEGS 63 Query: 218 VLLTESNAIAYYVA----NESLRGGDLATQARVWQWASWSDSELLPASCAWVFPYLGIMQ 385 V ESNAIA YV+ + SL G L A++ QW +S E+ + W P +G M Sbjct: 64 VF--ESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDFSSLEIYASILRWFGPRMGFMP 121 Query: 386 FNKQNVERAKSDLLAALKVLDGHLLTRTFL 475 ++ E A S L AL L+ HL + T+L Sbjct: 122 YSAPAEEGAISTLKRALDALNTHLTSNTYL 151 Score = 43.2 bits (97), Expect = 2e-04 Identities = 25/69 (36%), Positives = 34/69 (49%) Frame = +3 Query: 474 LVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCA 653 LV ITLAD+I L F V+ S +V+R+F TV +QP + V+G + Sbjct: 151 LVGHSITLADIITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLGDVKQTE 210 Query: 654 APPTYDPKK 680 A P KK Sbjct: 211 AVPPIASKK 219 >At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / eEF-1B gamma, putative similar to elongation factor 1B gamma GI:3868758 from [Oryza sativa] Length = 413 Score = 104 bits (250), Expect = 7e-23 Identities = 61/150 (40%), Positives = 83/150 (55%), Gaps = 4/150 (2%) Frame = +2 Query: 38 VLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGK 217 V++TY N A KALIAA+Y+G ++ + +F G TNKS +FLK P GKVP E+ +G Sbjct: 4 VMHTYKGNKGANKALIAAEYAGVKIEESADFQMGVTNKSPEFLKMNPIGKVPVLETPEGP 63 Query: 218 VLLTESNAIAYYVA----NESLRGGDLATQARVWQWASWSDSELLPASCAWVFPYLGIMQ 385 + ESNAIA YV+ + SL G L A + QW +S E+ W P +G Sbjct: 64 IF--ESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDFSSLEIDANMLKWFAPRMGYAP 121 Query: 386 FNKQNVERAKSDLLAALKVLDGHLLTRTFL 475 F+ E A S L L+ L+ HL + TFL Sbjct: 122 FSAPAEEAAISALKRGLEALNTHLASNTFL 151 Score = 41.9 bits (94), Expect = 5e-04 Identities = 22/69 (31%), Positives = 34/69 (49%) Frame = +3 Query: 474 LVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCA 653 LV +TLAD++ L F V+ S+ +V+R+F T+ +QP+ V+G Sbjct: 151 LVGHSVTLADIVTICNLNLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTE 210 Query: 654 APPTYDPKK 680 A P KK Sbjct: 211 AVPPVPTKK 219 >At1g02930.1 68414.m00260 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 208 Score = 46.4 bits (105), Expect = 2e-05 Identities = 30/93 (32%), Positives = 47/93 (50%), Gaps = 1/93 (1%) Frame = +2 Query: 26 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 205 MA ++ +P + + LIA D + + +K E F+ + P GKVPAFE Sbjct: 1 MAGIKVFGHPASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFED 60 Query: 206 ADGKVLLTESNAIAYYVANE-SLRGGDLATQAR 301 D K+ ES AI Y+A+E S +G +L + + Sbjct: 61 GDFKIF--ESRAITQYIAHEFSDKGNNLLSTGK 91 >At4g02520.1 68417.m00345 glutathione S-transferase, putative Length = 212 Score = 44.8 bits (101), Expect = 7e-05 Identities = 27/79 (34%), Positives = 41/79 (51%) Frame = +2 Query: 26 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 205 MA ++ +P + + LIA D ++ + +K E FL + P G+VPAFE Sbjct: 1 MAGIKVFGHPASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE- 59 Query: 206 ADGKVLLTESNAIAYYVAN 262 DG + L ES AI Y+A+ Sbjct: 60 -DGDLKLFESRAITQYIAH 77 >At2g02930.1 68415.m00241 glutathione S-transferase, putative Length = 212 Score = 44.0 bits (99), Expect = 1e-04 Identities = 27/79 (34%), Positives = 41/79 (51%) Frame = +2 Query: 26 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 205 MA ++ +P + + LIA D ++ + +K E FL + P G+VPAFE Sbjct: 1 MAGIKVFGHPASTSTRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFE- 59 Query: 206 ADGKVLLTESNAIAYYVAN 262 DG + L ES AI Y+A+ Sbjct: 60 -DGDLKLFESRAITQYIAH 77 >At1g02920.1 68414.m00259 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus]; supported by cDNA GI:443697. Length = 209 Score = 41.9 bits (94), Expect = 5e-04 Identities = 27/79 (34%), Positives = 39/79 (49%) Frame = +2 Query: 26 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 205 MA ++ +P + + LIA D + + +K E F+ + P GKVPAFE Sbjct: 1 MAGIKVFGHPASTATRRVLIALHEKNLDFEFVHIELKDGEHKKEPFIFRNPFGKVPAFE- 59 Query: 206 ADGKVLLTESNAIAYYVAN 262 DG L ES AI Y+A+ Sbjct: 60 -DGDFKLFESRAITQYIAH 77 >At2g02380.1 68415.m00176 glutathione S-transferase, putative similar to gi:167970 gb:AAA72320 gb:AY052332 Length = 223 Score = 39.5 bits (88), Expect = 0.003 Identities = 27/73 (36%), Positives = 39/73 (53%), Gaps = 1/73 (1%) Frame = +2 Query: 41 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGK 217 LY+Y + A++ IA G D + P N + G+ + S DF K P G VPA DG Sbjct: 14 LYSYWRSSCAHRVRIALTLKGLDYEYIPVNLLKGDQSDS-DFKKINPMGTVPAL--VDGD 70 Query: 218 VLLTESNAIAYYV 256 V++ +S AI Y+ Sbjct: 71 VVINDSFAIIMYL 83 >At1g02950.2 68414.m00263 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 245 Score = 38.3 bits (85), Expect = 0.006 Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%) Frame = +2 Query: 146 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 286 +K+E FL P G+VP FE DG V L ES AI Y+A S RG L Sbjct: 64 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 109 >At1g02950.1 68414.m00262 glutathione S-transferase, putative similar to glutathione-S-transferase GI:169887 from [Silene vulgaris] Length = 243 Score = 38.3 bits (85), Expect = 0.006 Identities = 24/48 (50%), Positives = 28/48 (58%), Gaps = 1/48 (2%) Frame = +2 Query: 146 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN-ESLRGGDL 286 +K+E FL P G+VP FE DG V L ES AI Y+A S RG L Sbjct: 62 HKTEPFLSLNPFGQVPVFE--DGSVKLYESRAITQYIAYVHSSRGTQL 107 >At1g02940.1 68414.m00261 glutathione S-transferase, putative similar to glutathione S-transferase GI:860955 from [Hyoscyamus muticus] Length = 256 Score = 38.3 bits (85), Expect = 0.006 Identities = 25/74 (33%), Positives = 35/74 (47%), Gaps = 1/74 (1%) Frame = +2 Query: 41 LYTYPENFRAYKALIAAQYSGTDVK-VAPNFVFGETNKSEDFLKKFPAGKVPAFESADGK 217 +Y YP + + L G + N + G+ K FL P G+VP F DG Sbjct: 41 IYGYPYSTNTRRVLAVLHEKGLSYDPITVNLIAGD-QKKPSFLAINPFGQVPVF--LDGG 97 Query: 218 VLLTESNAIAYYVA 259 + LTES AI+ Y+A Sbjct: 98 LKLTESRAISEYIA 111 >At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 191 Score = 37.9 bits (84), Expect = 0.009 Identities = 27/73 (36%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Frame = +2 Query: 41 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGK 217 LY+Y + A++ IA G D + P N + G+ S DF K P G VPA DG Sbjct: 11 LYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGD 67 Query: 218 VLLTESNAIAYYV 256 V++ +S AI Y+ Sbjct: 68 VVINDSFAIIMYL 80 >At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 221 Score = 37.9 bits (84), Expect = 0.009 Identities = 27/73 (36%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Frame = +2 Query: 41 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKSEDFLKKFPAGKVPAFESADGK 217 LY+Y + A++ IA G D + P N + G+ S DF K P G VPA DG Sbjct: 11 LYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDS-DFKKINPMGTVPAL--VDGD 67 Query: 218 VLLTESNAIAYYV 256 V++ +S AI Y+ Sbjct: 68 VVINDSFAIIMYL 80 >At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.-) (MAI) {Arabidopsis thaliana}; contains Pfam profiles PF02798: Glutathione S-transferase, N-terminal domain and PF00043:Glutathione S-transferase, C-terminal domain Length = 228 Score = 34.7 bits (76), Expect = 0.080 Identities = 27/79 (34%), Positives = 39/79 (49%), Gaps = 7/79 (8%) Frame = +2 Query: 41 LYTYPENFRAYKALIAAQYSGTDVKVAP-NFVFGETNKS------EDFLKKFPAGKVPAF 199 LY+Y + A++ IA G D + P N + G+ S +DF K P G VPA Sbjct: 11 LYSYWRSSCAHRVRIALALKGLDYEYIPVNLLKGDQFDSVYRFDLQDFKKINPMGTVPAL 70 Query: 200 ESADGKVLLTESNAIAYYV 256 DG V++ +S AI Y+ Sbjct: 71 --VDGDVVINDSFAIIMYL 87 >At1g16840.2 68414.m02028 expressed protein Length = 161 Score = 31.9 bits (69), Expect = 0.56 Identities = 26/80 (32%), Positives = 34/80 (42%) Frame = +3 Query: 210 MERCF*LKAMPSLTTLPMKVSAEEIWLPKPVSGSGHHGLTANYCLLPALGSSLTLVSCNS 389 + R F +A P LP V W+P P SGS HG+ L+ L + LT S Sbjct: 67 VHRIFVRRAQPDW--LPF-VPGASYWVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLS 120 Query: 390 TNRMLNVQSLTYWPP*KYWT 449 TN S Y+ YW+ Sbjct: 121 TNSSHGWPSSDYFLKGSYWS 140 >At5g46550.1 68418.m05731 DNA-binding bromodomain-containing protein contains bromodomain, INTERPRO:IPR001487 Length = 494 Score = 29.5 bits (63), Expect = 3.0 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = +2 Query: 77 ALIAAQYSGTDVKVAPNFVFGETNKSE 157 A++ AQY+GT +K V G+ NK++ Sbjct: 342 AILKAQYAGTIIKAKHRIVLGQNNKAD 368 >At3g14270.1 68416.m01806 phosphatidylinositol-4-phosphate 5-kinase family protein similar to SP|Q9Z1T6 FYVE finger-containing phosphoinositide kinase (EC 2.7.1.68) (1- phosphatidylinositol-4-phosphate kinase) (PIP5K) (PtdIns(4)P-5-kinase) {Mus musculus}; contains Pfam profiles PF01504: Phosphatidylinositol-4-phosphate 5-Kinase, PF01363: FYVE zinc finger, PF00118: TCP-1/cpn60 chaperonin family Length = 1791 Score = 29.5 bits (63), Expect = 3.0 Identities = 31/109 (28%), Positives = 45/109 (41%) Frame = +2 Query: 14 KTPTMAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVP 193 K+PT A +L Y A K L + + + DV + N +FG T K LK + Sbjct: 1581 KSPTCLAKILGIYQV---ATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRARYN 1637 Query: 194 AFESADGKVLLTESNAIAYYVANESLRGGDLATQARVWQWASWSDSELL 340 S KVLL + N I + G R+ + A W+D+ L Sbjct: 1638 PDSSGSNKVLL-DQNLIEAMPTSPIFVGN---KAKRLLERAVWNDTAFL 1682 >At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-containing protein-related contains weak hit to Pfam F00627: UBA/TS-N domain; supported by tandem duplication of ubiquitin-associated (UBA)/TS-N domain protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis thaliana] Length = 508 Score = 29.5 bits (63), Expect = 3.0 Identities = 12/38 (31%), Positives = 18/38 (47%) Frame = +3 Query: 372 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLVTE 485 L++ + N N +S + WPP K W D H + E Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICHETKVSRE 232 >At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / long-chain acyl-CoA synthetase nearly identical to acyl CoA synthetase (MF45P) GI:1617268 from [Brassica napus] Length = 665 Score = 29.5 bits (63), Expect = 3.0 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = -3 Query: 577 FISDERTLGSSTCWKACSSVLKTMTSASVILSVTKEGA 464 F+ ++ +C K CSS LKT+ S + S KE A Sbjct: 156 FVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEA 193 >At3g49210.1 68416.m05378 expressed protein Length = 518 Score = 29.1 bits (62), Expect = 4.0 Identities = 16/64 (25%), Positives = 30/64 (46%) Frame = +2 Query: 281 DLATQARVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLL 460 D+ + +W +W + P S L ++ K+ ++R K+ L AAL + G + Sbjct: 352 DMMAKGSTCRWGNWIGYIVFPFSIGLRDDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFI 411 Query: 461 TRTF 472 +TF Sbjct: 412 LKTF 415 >At4g22130.1 68417.m03199 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 338 Score = 28.7 bits (61), Expect = 5.2 Identities = 21/74 (28%), Positives = 35/74 (47%), Gaps = 6/74 (8%) Frame = +2 Query: 65 RAYKALIAAQYSGTDVKVAPNF-----VFGETNKSEDFLKKFPAGKVPAFESADGKVL-L 226 R + A+QY+ + ++VA N + GE + + +FP GK+ A + D L L Sbjct: 8 RIRSPITASQYTVSSLQVATNSFSQENIIGEGSLGRVYRAEFPNGKIMAIKKIDNAALSL 67 Query: 227 TESNAIAYYVANES 268 E + V+N S Sbjct: 68 QEEDNFLEAVSNMS 81 >At5g20930.1 68418.m02486 protein kinase, putative nearly identical to protein kinase tousled gi|433052|gb|AAA32874 Length = 688 Score = 27.9 bits (59), Expect = 9.1 Identities = 9/37 (24%), Positives = 24/37 (64%) Frame = -2 Query: 116 LSHQSRNIVRRSTLYKRGSFPDKCKVPRPPWLEFSER 6 +S+++++++RR Y + PD + + P+L +S++ Sbjct: 652 ISNEAKDLIRRCLTYNQEDRPDVLTMAQDPYLAYSKK 688 >At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like transposon protein GB:AAB95292 GI:2088658 from [Arabidopsis thaliana] Length = 1148 Score = 27.9 bits (59), Expect = 9.1 Identities = 14/28 (50%), Positives = 18/28 (64%) Frame = -1 Query: 639 ASRRRPTLAAGGRRSGTNAERLSATNGR 556 +S R P+ A GRRSGT R+S T G+ Sbjct: 171 SSARHPS-PASGRRSGTPVRRISPTPGK 197 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,906,067 Number of Sequences: 28952 Number of extensions: 413445 Number of successful extensions: 1183 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 1142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1183 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1999652000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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