BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_I05 (847 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|c... 236 3e-63 SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||M... 175 7e-45 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 69 1e-12 SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 59 9e-10 SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 44 2e-05 SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosacch... 40 3e-04 SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 35 0.017 SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 34 0.029 SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 31 0.27 SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 31 0.27 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 28 1.9 SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12 |Schizos... 27 3.3 SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 27 3.3 SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 27 4.4 SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces po... 27 4.4 SPBC1778.02 |rap1||telomere binding protein Rap1|Schizosaccharom... 27 4.4 SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein... 26 5.8 SPCC188.12 |spn6|SPCC584.09|septin Spn6|Schizosaccharomyces pomb... 26 7.7 SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|c... 26 7.7 SPBC2D10.15c |||peptidyl-tRNA hydrolase Pth1 |Schizosaccharomyce... 26 7.7 >SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 236 bits (577), Expect = 3e-63 Identities = 121/246 (49%), Positives = 165/246 (67%), Gaps = 7/246 (2%) Frame = +2 Query: 122 GRVGTNLKVGIVGVPNVGKSTFFNVLTKSQAA-AENFPFCTIDPNESRVPVPDERYDYLC 298 GR G NLK GIVG+PNVGKSTFF +TKS N+P+ TIDP E++V VPDER+D+LC Sbjct: 15 GRPGNNLKTGIVGMPNVGKSTFFRAITKSVLGNPANYPYATIDPEEAKVAVPDERFDWLC 74 Query: 299 EYHKPASKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIFNLCRAFDDEDVIHV 478 E +KP S+VPAFL V DIAGL +GA+ G GLGNAFLSH++A DAI+ + RAFDD ++IHV Sbjct: 75 EAYKPKSRVPAFLTVFDIAGLTKGASTGVGLGNAFLSHVRAVDAIYQVVRAFDDAEIIHV 134 Query: 479 DGDVNPVRDLETIAEELRLKDEEQLLQHIEKLDRVVNRGG---EKKLKPEYDS-LAKVKT 646 +GDV+P+RDL I +EL +KD E + +H+E L ++ +RG E K K E + + KV Sbjct: 135 EGDVDPIRDLSIIVDELLIKDAEFVEKHLEGLRKITSRGANTLEMKAKKEEQAIIEKVYQ 194 Query: 647 ILVDEKKHIXFGDWSAADIEV*INTCS*L--QNPLYTSXIYLKRTTLXKRTXGXKAQEWI 820 L + K+ I GDWS ++E+ IN+ L + +Y + + K K ++WI Sbjct: 195 YLTETKQPIRKGDWSNREVEI-INSLYLLTAKPVIYLVNMSERDFLRQKNKYLPKIKKWI 253 Query: 821 DKNDPG 838 D+N PG Sbjct: 254 DENSPG 259 >SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 407 Score = 175 bits (426), Expect = 7e-45 Identities = 93/243 (38%), Positives = 145/243 (59%), Gaps = 3/243 (1%) Frame = +2 Query: 119 IGRVGTNLKVGIVGVPNVGKSTFFNVLTKSQAA-AENFPFCTIDPNESRVPVPDERYDYL 295 +GR G +LK+GIVG+PN+GKST F +LTK+ N+PF TIDP ++ PV D +Y+ L Sbjct: 39 LGRPGNHLKIGIVGMPNIGKSTLFQILTKTNLGNPANYPFATIDPVHAKAPVLDSQYELL 98 Query: 296 CEYHKPASKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIFNLCRAFDDEDVIH 475 CE ++P +++PA L + D AGL R +++G+GLGNAFLS+I++ DA+F L RAF + V H Sbjct: 99 CEIYQPKTRIPAQLTIYDTAGLTRNSSKGEGLGNAFLSNIRSVDALFQLVRAFPEAQVPH 158 Query: 476 VDGDVNPVRDLETIAEELRLKDEEQLLQHIEKLDRVVNRGGEKKLKPEYDSLAKVKTILV 655 V+ V+PVRDL+ I EEL LKD E L +++K R K D+ + +++ Sbjct: 159 VEKSVDPVRDLQIIQEELLLKDAEFLESYLKKEGR-----SPKTCAKALDTARRALEVVL 213 Query: 656 DEKKHIXFGDWSAADIEV*INTCS*LQNPLYTSXIYLKRTT-LXKRTXGXKA-QEWIDKN 829 + + I +W+ I + +N+ + L + + + L K +EW++KN Sbjct: 214 GKGRQISKAEWNDEQIPI-LNSLNLLTAKPVVYLVNMDQDDYLSDEQEALKGIKEWVEKN 272 Query: 830 DPG 838 G Sbjct: 273 SFG 275 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 68.5 bits (160), Expect = 1e-12 Identities = 40/116 (34%), Positives = 61/116 (52%), Gaps = 11/116 (9%) Frame = +2 Query: 128 VGTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTIDPNE-----------SRVPVP 274 +G ++ +G VG P+ GKST N LT + A NFPF TI+PN SR + Sbjct: 1 MGRDILIGFVGKPSSGKSTMLNALTDATAKTGNFPFTTIEPNRAIGYAQIECACSRFGLQ 60 Query: 275 DERYDYLCEYHKPASKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIFNL 442 D+ +P + ++D+AGL+ GA G+GLGN FL ++ DA+ ++ Sbjct: 61 DKCKPIYGGCKNGVRSIP--IQLLDVAGLIPGAHAGKGLGNKFLDDLRHADALVHV 114 >SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 58.8 bits (136), Expect = 9e-10 Identities = 33/97 (34%), Positives = 54/97 (55%), Gaps = 2/97 (2%) Frame = +2 Query: 134 TNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTIDP--NESRVPVPDERYDYLCEYH 307 T ++G+VG+PN GKST N LT S++ + F TI P + +PD+ + +Y Sbjct: 235 TICEIGLVGLPNAGKSTLLNCLTASKSKVGEYEFTTIYPKIGTIKTTMPDDHSSF--QY- 291 Query: 308 KPASKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIK 418 + DI G+++GA++G+GLG FL H++ Sbjct: 292 ----------RLADIPGIIKGASDGKGLGYDFLRHVE 318 >SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 366 Score = 44.4 bits (100), Expect = 2e-05 Identities = 30/106 (28%), Positives = 53/106 (50%) Frame = +2 Query: 119 IGRVGTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTIDPNESRVPVPDERYDYLC 298 + R G VG +G P+VGKST LT +++ A + F T+ VP + Sbjct: 58 VARTGIGT-VGFIGFPSVGKSTLMTQLTGTRSEAAAYEFTTL------TTVPG-----VL 105 Query: 299 EYHKPASKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIF 436 +Y+ A + ++D+ G++ GA +G+G G ++ + C+ IF Sbjct: 106 QYN------GAKIQILDLPGIIEGAKDGRGRGKQVITVARTCNLIF 145 >SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 40.3 bits (90), Expect = 3e-04 Identities = 28/97 (28%), Positives = 52/97 (53%) Frame = +2 Query: 143 KVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTIDPNESRVPVPDERYDYLCEYHKPASK 322 +V +G P+VGKST + +TK+++A ++ F T+ + +P E YD Sbjct: 64 RVAFIGFPSVGKSTLLSAITKTKSATASYEFTTL----TAIPGVLE-YD----------- 107 Query: 323 VPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAI 433 A + ++D+ G++ GA++G+G G +S + D I Sbjct: 108 -GAEIQMLDLPGIIEGASQGRG-GRQAVSAARTADLI 142 >SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 34.7 bits (76), Expect = 0.017 Identities = 14/36 (38%), Positives = 22/36 (61%) Frame = +2 Query: 125 RVGTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFP 232 ++ ++L VG++G PNVGKS+ N L A + P Sbjct: 265 KLKSSLTVGVIGYPNVGKSSVINALVNRSANGRSAP 300 >SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 33.9 bits (74), Expect = 0.029 Identities = 13/30 (43%), Positives = 20/30 (66%) Frame = +2 Query: 152 IVGVPNVGKSTFFNVLTKSQAAAENFPFCT 241 + G PNVGKS+F N +T++Q + + F T Sbjct: 173 VCGYPNVGKSSFMNKVTRAQVDVQPYAFTT 202 >SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 30.7 bits (66), Expect = 0.27 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +2 Query: 140 LKVGIVGVPNVGKSTFFNVLTKSQ 211 + VG++G PN GKS+ N L K + Sbjct: 311 ISVGLIGFPNAGKSSIINTLRKKK 334 >SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual Length = 616 Score = 30.7 bits (66), Expect = 0.27 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = +2 Query: 134 TNLKVGIVGVPNVGKSTFFNVLTKSQ 211 T + G+VG PNVGKS+ N L S+ Sbjct: 300 TKMTFGLVGYPNVGKSSTINALVGSK 325 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 27.9 bits (59), Expect = 1.9 Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 4/41 (9%) Frame = +2 Query: 143 KVGIVGVPNVGKSTFFNVLTKSQAA----AENFPFCTIDPN 253 KV IVG+ GKST +L K+ + + +P ID N Sbjct: 1130 KVAIVGISGSGKSTLVELLRKTYPSEDIYIDGYPLTNIDTN 1170 >SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 27.1 bits (57), Expect = 3.3 Identities = 14/54 (25%), Positives = 29/54 (53%) Frame = +2 Query: 509 ETIAEELRLKDEEQLLQHIEKLDRVVNRGGEKKLKPEYDSLAKVKTILVDEKKH 670 E++ E+ K EEQ +H+E L V + + + + + V+ IL++ +K+ Sbjct: 157 ESLLEDFEAKIEEQQHKHLENLGDEVEQDWKPLVIEQIPTRDDVEAILLERRKN 210 >SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 506 Score = 27.1 bits (57), Expect = 3.3 Identities = 13/45 (28%), Positives = 23/45 (51%) Frame = +2 Query: 539 DEEQLLQHIEKLDRVVNRGGEKKLKPEYDSLAKVKTILVDEKKHI 673 ++ QL + +EK+ R + G E L ++ L LVDE+ + Sbjct: 179 EKTQLSEKVEKMKRSTDLGAEAVLPLDFQDLRVALLSLVDERNEM 223 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 26.6 bits (56), Expect = 4.4 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +2 Query: 137 NLKVGIVGVPNVGKSTFFNVLTKSQAA 217 ++K+ IVG NVG +T F +L AA Sbjct: 20 SIKIVIVGAGNVGSTTAFTLLLSGLAA 46 >SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces pombe|chr 1|||Manual Length = 743 Score = 26.6 bits (56), Expect = 4.4 Identities = 15/47 (31%), Positives = 20/47 (42%) Frame = +2 Query: 302 YHKPASKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIFNL 442 Y+ K P FL V G V G + N + ++A IFNL Sbjct: 570 YYTDEEKPPKFLPWVSFIGFVLGIIWISTIANEVVGILRALGVIFNL 616 >SPBC1778.02 |rap1||telomere binding protein Rap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 693 Score = 26.6 bits (56), Expect = 4.4 Identities = 16/40 (40%), Positives = 21/40 (52%) Frame = +2 Query: 485 DVNPVRDLETIAEELRLKDEEQLLQHIEKLDRVVNRGGEK 604 DVN D E L+DE+ L+ H+ K D +NR G K Sbjct: 111 DVNRTHDGFRKRELFTLEDEKILIDHVHKND--INRFGTK 148 >SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 758 Score = 26.2 bits (55), Expect = 5.8 Identities = 17/43 (39%), Positives = 20/43 (46%), Gaps = 1/43 (2%) Frame = +2 Query: 116 LIGRV-GTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCT 241 L RV T+ K+ I G N GKST N L E+ CT Sbjct: 146 LFSRVEDTSSKILITGDLNAGKSTLCNALVHKDILPEDQQPCT 188 >SPCC188.12 |spn6|SPCC584.09|septin Spn6|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 25.8 bits (54), Expect = 7.7 Identities = 12/28 (42%), Positives = 15/28 (53%) Frame = +2 Query: 116 LIGRVGTNLKVGIVGVPNVGKSTFFNVL 199 LI R L + + G GK+TFFN L Sbjct: 23 LIKRKECGLTIMLCGASGTGKTTFFNTL 50 >SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 433 Score = 25.8 bits (54), Expect = 7.7 Identities = 15/39 (38%), Positives = 22/39 (56%) Frame = +2 Query: 389 LGNAFLSHIKACDAIFNLCRAFDDEDVIHVDGDVNPVRD 505 LG + S KA DAI L + F+D D V ++NP+ + Sbjct: 196 LGFSSSSKPKAVDAIVKLYKVFNDCDATQV--EINPLAE 232 >SPBC2D10.15c |||peptidyl-tRNA hydrolase Pth1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 218 Score = 25.8 bits (54), Expect = 7.7 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = -2 Query: 450 ARHKLKIASQALMWDRKAFPNPWPSAAPRTRPAMSTT 340 +RH L ++ D AFP WPS +ST+ Sbjct: 46 SRHSLGKLMVSMYADSMAFPKNWPSTKENLTLVLSTS 82 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,986,047 Number of Sequences: 5004 Number of extensions: 56726 Number of successful extensions: 213 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 200 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 207 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 418457710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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