BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_H10 (853 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 161 5e-40 SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 157 1e-38 SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 151 7e-37 SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 146 2e-35 SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 88 7e-18 SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 87 1e-17 SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 86 4e-17 SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 59 4e-09 SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.) 44 2e-04 SB_52505| Best HMM Match : DUF963 (HMM E-Value=0.81) 30 2.7 SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) 29 4.8 SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) 29 4.8 SB_23942| Best HMM Match : 5-nucleotidase (HMM E-Value=4) 29 6.3 SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 6.3 SB_4738| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 6.3 SB_50521| Best HMM Match : MFS_1 (HMM E-Value=0.0026) 28 8.4 >SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) Length = 640 Score = 161 bits (392), Expect = 5e-40 Identities = 86/140 (61%), Positives = 98/140 (70%), Gaps = 1/140 (0%) Frame = +2 Query: 425 KPFSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLR 604 K F+ + L KMKETAEAYLG+ V +AVITVPAYFNDSQRQATKDAGTI+GLNVLR Sbjct: 112 KTFAAEEISSMVLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLR 171 Query: 605 IINEPTAAAIAYGLDKKGTGERNVLIFDL-GXRYLRRVHPYHRGWYLRGEIHRRRXHLGG 781 +INEPTAAA+AYGLDK +GE+NVLIFDL G + + G + HLGG Sbjct: 172 VINEPTAAALAYGLDKSLSGEKNVLIFDLGGGTFDVSILTIDDGSLFEVKSTAGDTHLGG 231 Query: 782 EDFDNRXXXHFVQEFXRXSK 841 EDFDNR HFV EF R K Sbjct: 232 EDFDNRLVNHFVAEFKRKYK 251 Score = 140 bits (340), Expect = 1e-33 Identities = 62/82 (75%), Positives = 73/82 (89%) Frame = +1 Query: 211 YVAFTDTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGK 390 YV FTDTERL+GDAAKNQVA+NP NTIFDAKRLIGR+++DATVQ+DMK WPF+++SD K Sbjct: 41 YVGFTDTERLVGDAAKNQVALNPENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNK 100 Query: 391 PKIKVAYKGEDKTFFPEEVSSM 456 PKI+V YKGE KTF EE+SSM Sbjct: 101 PKIQVEYKGERKTFAAEEISSM 122 Score = 70.9 bits (166), Expect = 1e-12 Identities = 31/36 (86%), Positives = 34/36 (94%) Frame = +3 Query: 90 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGQQ 197 M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQG + Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNR 36 >SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1097 Score = 157 bits (380), Expect = 1e-38 Identities = 86/140 (61%), Positives = 99/140 (70%), Gaps = 1/140 (0%) Frame = +2 Query: 425 KPFSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLR 604 K F P + LTKMKETAEAYLG V +AV+TVPAYF+DSQRQATKDAGTI+GLNVLR Sbjct: 112 KSFFPEEISSMVLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLR 171 Query: 605 IINEPTAAAIAYGLDKKGTGERNVLIFDLGX-RYLRRVHPYHRGWYLRGEIHRRRXHLGG 781 IINEPTAAAIAYGLDKK ERNVLI+DLG + V G + + HLGG Sbjct: 172 IINEPTAAAIAYGLDKKVGVERNVLIYDLGGGTFDVSVLTIEDGIF-EVKSTAGDTHLGG 230 Query: 782 EDFDNRXXXHFVQEFXRXSK 841 EDFDN+ HFV++F + K Sbjct: 231 EDFDNKMVDHFVKQFKQKYK 250 Score = 139 bits (337), Expect = 2e-33 Identities = 61/82 (74%), Positives = 71/82 (86%) Frame = +1 Query: 211 YVAFTDTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGK 390 YVAF+DTERLIGDAAKNQVAMNP NT+FDAKRLIGRKF+D V DM HWPF V+ +G + Sbjct: 41 YVAFSDTERLIGDAAKNQVAMNPENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGER 100 Query: 391 PKIKVAYKGEDKTFFPEEVSSM 456 PKI+V +KGE K+FFPEE+SSM Sbjct: 101 PKIQVEFKGEKKSFFPEEISSM 122 Score = 74.1 bits (174), Expect = 1e-13 Identities = 33/36 (91%), Positives = 34/36 (94%) Frame = +3 Query: 90 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGQQ 197 MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQG + Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNR 36 Score = 54.4 bits (125), Expect = 1e-07 Identities = 24/25 (96%), Positives = 25/25 (100%) Frame = +3 Query: 693 GGGTFDVSILTIEDGIFEVKSTAGD 767 GGGTFDVS+LTIEDGIFEVKSTAGD Sbjct: 201 GGGTFDVSVLTIEDGIFEVKSTAGD 225 >SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 746 Score = 151 bits (366), Expect = 7e-37 Identities = 84/128 (65%), Positives = 91/128 (71%), Gaps = 1/128 (0%) Frame = +2 Query: 461 LTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAY 640 L KMKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG ISGLNVLRIINEPTAAAIAY Sbjct: 217 LNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPTAAAIAY 276 Query: 641 GLDKKGTGERNVLIFDL-GXRYLRRVHPYHRGWYLRGEIHRRRXHLGGEDFDNRXXXHFV 817 GLDKK ERNVLIFDL G + V G + H HLGGEDFDN FV Sbjct: 277 GLDKKVGQERNVLIFDLGGGTFDVSVLSIEDGIFEVKSTH-GDTHLGGEDFDNNMVDFFV 335 Query: 818 QEFXRXSK 841 ++F + K Sbjct: 336 KQFKQKYK 343 Score = 136 bits (330), Expect = 2e-32 Identities = 61/79 (77%), Positives = 69/79 (87%) Frame = +1 Query: 211 YVAFTDTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGK 390 YVAFTD ERL+GDAAKNQVAMNP NTIFDAKRLIGR+F+D V+ DMKHW FEVV++ G+ Sbjct: 41 YVAFTDEERLVGDAAKNQVAMNPTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGR 100 Query: 391 PKIKVAYKGEDKTFFPEEV 447 PK+KV YKGE KTFF EEV Sbjct: 101 PKVKVEYKGETKTFFAEEV 119 Score = 73.7 bits (173), Expect = 2e-13 Identities = 32/36 (88%), Positives = 34/36 (94%) Frame = +3 Query: 90 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGQQ 197 M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQG + Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNR 36 Score = 72.9 bits (171), Expect = 3e-13 Identities = 36/53 (67%), Positives = 40/53 (75%) Frame = +2 Query: 419 KTKPFSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAG 577 +TK F + L KMKETAEAYLG V +AV+TVPAYFNDSQRQATKDAG Sbjct: 135 ETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAG 187 >SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) Length = 1327 Score = 146 bits (354), Expect = 2e-35 Identities = 79/154 (51%), Positives = 99/154 (64%), Gaps = 2/154 (1%) Frame = +2 Query: 377 VMEANLRSR*HIRVKT--KPFSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDS 550 V++ N + ++VK K F+ + LTKMKETAEAYLGK V +AV+TVPAYFND+ Sbjct: 792 VVDKNKKPHVQVKVKDEIKTFAAEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDA 851 Query: 551 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGXRYLRRVHPYHR 730 QRQATKDAG I+GL V+RIINEPTAAAIAYG+DKK GE+N+L+FDLG Sbjct: 852 QRQATKDAGAIAGLTVMRIINEPTAAAIAYGMDKK-EGEKNILVFDLGGGTFDVSLLTID 910 Query: 731 GWYLRGEIHRRRXHLGGEDFDNRXXXHFVQEFXR 832 HLGGEDFD HF++ + + Sbjct: 911 NGVFEVVATNGDTHLGGEDFDQNVMEHFIKLYKK 944 Score = 103 bits (246), Expect = 2e-22 Identities = 49/83 (59%), Positives = 62/83 (74%), Gaps = 1/83 (1%) Frame = +1 Query: 211 YVAFTDT-ERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG 387 YVAFT+ ERLIGD AKNQ+ NP NT+FDAKRLIGR ++D +VQ D+K +PF+VV Sbjct: 738 YVAFTEEGERLIGDGAKNQLTTNPENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNK 797 Query: 388 KPKIKVAYKGEDKTFFPEEVSSM 456 KP ++V K E KTF EE+S+M Sbjct: 798 KPHVQVKVKDEIKTFAAEEISAM 820 Score = 58.0 bits (134), Expect = 9e-09 Identities = 24/30 (80%), Positives = 29/30 (96%) Frame = +3 Query: 108 VGIDLGTTYSCVGVFQHGKVEIIANDQGQQ 197 +GIDLGTTYSCVGVF++G+VEIIANDQG + Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNR 733 Score = 44.0 bits (99), Expect = 2e-04 Identities = 18/25 (72%), Positives = 23/25 (92%) Frame = +3 Query: 693 GGGTFDVSILTIEDGIFEVKSTAGD 767 GGGTFDVS+LTI++G+FEV +T GD Sbjct: 898 GGGTFDVSLLTIDNGVFEVVATNGD 922 >SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) Length = 179 Score = 88.2 bits (209), Expect = 7e-18 Identities = 56/109 (51%), Positives = 68/109 (62%), Gaps = 26/109 (23%) Frame = +2 Query: 425 KPFSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLN--- 595 + F+P + L ++K AEA+LG+ V+ AVITVPA+FND+QRQATKDAGTI+GL+ Sbjct: 11 RSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAGTIAGLDVLS 70 Query: 596 ---------------------VLRIINEPTAAAIAYGLDKK--GTGERN 673 VLRIINEPTAAAIAYGLDK G ERN Sbjct: 71 RTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDKARGGKAERN 119 >SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1068 Score = 87.4 bits (207), Expect = 1e-17 Identities = 51/139 (36%), Positives = 74/139 (53%), Gaps = 3/139 (2%) Frame = +2 Query: 419 KTKPFSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNV 598 K + FSP + + T++K TAE L V + VI+VP+Y+ D QR+ DA +GLN Sbjct: 167 KQEVFSPEQVMAMLFTRLKTTAEIALKTKVTDCVISVPSYYTDRQRRCMLDASATAGLNC 226 Query: 599 LRIINEPTAAAIAYGLDKKG---TGERNVLIFDLGXRYLRRVHPYHRGWYLRGEIHRRRX 769 LR++N+ TA ++AYG+ K+ RNV+ D+G L+ L+ Sbjct: 227 LRLMNDTTAVSLAYGIYKQDLPTDKPRNVVFVDIGHSSLQVCITAFLKGQLKVLSTAVEP 286 Query: 770 HLGGEDFDNRXXXHFVQEF 826 +LGG DFD HF QEF Sbjct: 287 NLGGRDFDYVLVEHFAQEF 305 Score = 52.4 bits (120), Expect = 4e-07 Identities = 27/62 (43%), Positives = 36/62 (58%), Gaps = 1/62 (1%) Frame = +1 Query: 274 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVS 450 N NTI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V Sbjct: 118 NLKNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVM 177 Query: 451 SM 456 +M Sbjct: 178 AM 179 >SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) Length = 437 Score = 85.8 bits (203), Expect = 4e-17 Identities = 51/129 (39%), Positives = 73/129 (56%) Frame = +2 Query: 434 SPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIIN 613 +P + + + K++ AE L TV AV++VPA F+ QR AT A +++G+ VLR+IN Sbjct: 145 TPEEIGGYVIKKLRTMAERNLTTTVTKAVMSVPAEFDIKQRNATVKAASLAGITVLRVIN 204 Query: 614 EPTAAAIAYGLDKKGTGERNVLIFDLGXRYLRRVHPYHRGWYLRGEIHRRRXHLGGEDFD 793 EPTAAA+AYGL KK G NV++ DLG L +G LGG+DF+ Sbjct: 205 EPTAAALAYGLHKK-EGVANVMVVDLGGGTLDVSLLNIQGGMFVTMAMAGNNRLGGQDFN 263 Query: 794 NRXXXHFVQ 820 R + +Q Sbjct: 264 ARFMQYLLQ 272 Score = 57.2 bits (132), Expect = 2e-08 Identities = 32/79 (40%), Positives = 42/79 (53%), Gaps = 1/79 (1%) Frame = +1 Query: 214 VAFTDTERLIGDAAKNQVAMNPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSD-GGK 390 VAFTD L+G+ A +Q +NP NTI+DAKR IG+ F + K + F V +D G Sbjct: 71 VAFTDKGVLVGEKALSQAEINPKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGN 130 Query: 391 PKIKVAYKGEDKTFFPEEV 447 V KG PEE+ Sbjct: 131 AGFLVGKKGNFTVITPEEI 149 Score = 38.7 bits (86), Expect = 0.006 Identities = 16/32 (50%), Positives = 24/32 (75%), Gaps = 2/32 (6%) Frame = +3 Query: 96 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIAND 185 K +GIDLGTTYSCVG +Q G+++I +++ Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDE 61 >SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1019 Score = 59.3 bits (137), Expect = 4e-09 Identities = 30/92 (32%), Positives = 54/92 (58%), Gaps = 4/92 (4%) Frame = +2 Query: 431 FSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRII 610 F+P + + L + E + +++ V+TVP +FN ++R+A A + GLNVL+I+ Sbjct: 80 FTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLRAAELVGLNVLQIM 139 Query: 611 NEPTAAAIAYGLDKK----GTGERNVLIFDLG 694 N TA A+ YGL ++ T E++ + +D+G Sbjct: 140 NSNTAVALNYGLFQQKSFNDTLEKHFMFYDMG 171 >SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 766 Score = 44.0 bits (99), Expect = 2e-04 Identities = 18/25 (72%), Positives = 22/25 (88%) Frame = +3 Query: 693 GGGTFDVSILTIEDGIFEVKSTAGD 767 GGGTFD+SIL I+ G+FEVK+T GD Sbjct: 8 GGGTFDISILEIQKGVFEVKATNGD 32 >SB_52505| Best HMM Match : DUF963 (HMM E-Value=0.81) Length = 388 Score = 29.9 bits (64), Expect = 2.7 Identities = 16/56 (28%), Positives = 28/56 (50%) Frame = -1 Query: 739 IPSSMVRMDTSKVPPPEVKDKYISFSSTLFVKTVSNRSSSRFIDDSENVQARDGTC 572 IPSS+V ++ ++P V + S+L + SSS F +S+ + + TC Sbjct: 319 IPSSLVTRESDRIPSSFVAFESDGIPSSLVTREFDGLSSSLFACESDGIPSSFVTC 374 >SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) Length = 420 Score = 29.1 bits (62), Expect = 4.8 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -1 Query: 295 RILCCWGSSPPGSWRH 248 R++CC G SP WRH Sbjct: 395 RVVCCQGDSPSPRWRH 410 >SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) Length = 340 Score = 29.1 bits (62), Expect = 4.8 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -1 Query: 295 RILCCWGSSPPGSWRH 248 R++CC G SP WRH Sbjct: 170 RVVCCQGDSPSPRWRH 185 >SB_23942| Best HMM Match : 5-nucleotidase (HMM E-Value=4) Length = 735 Score = 28.7 bits (61), Expect = 6.3 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = +2 Query: 665 ERNVLIFDLGXRYLRRVHPYHRGWYLRGE 751 +R VL FD +HP+ RGWY R E Sbjct: 521 KRAVLAFDELIMAAAGLHPFQRGWYRRCE 549 >SB_9053| Best HMM Match : TIG (HMM E-Value=0) Length = 2990 Score = 28.7 bits (61), Expect = 6.3 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = +3 Query: 36 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 122 HVC +V T K K ++K PA GI+L Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228 >SB_4738| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 67 Score = 28.7 bits (61), Expect = 6.3 Identities = 17/54 (31%), Positives = 25/54 (46%) Frame = +2 Query: 410 IRVKTKPFSPRKSVPWXLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATKD 571 +R KP+ P+K P+ L M + E Y KTV+ F +R+ T D Sbjct: 1 MRAGMKPYEPKKVPPFYLEDMLKMRE-YCLKTVETLYARRLIEFEKVERETTND 53 >SB_50521| Best HMM Match : MFS_1 (HMM E-Value=0.0026) Length = 1080 Score = 28.3 bits (60), Expect = 8.4 Identities = 22/76 (28%), Positives = 36/76 (47%), Gaps = 10/76 (13%) Frame = +2 Query: 467 KMKETAEAYLGKT----------VQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINE 616 K T+E Y+G T + NA +T+P YF + +K G G+ + +++ Sbjct: 997 KDNNTSETYVGLTENSFKTRRNKIYNAPLTLPPYFWCHPSRKSKKDGAKEGMAM--AMSK 1054 Query: 617 PTAAAIAYGLDKKGTG 664 I+Y LD+K TG Sbjct: 1055 ERNQEISYKLDRKWTG 1070 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 26,433,392 Number of Sequences: 59808 Number of extensions: 570220 Number of successful extensions: 1668 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 1497 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1659 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2419355818 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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