Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_H04
(851 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 250 2e-67
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 9e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 2e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 130 3e-31
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.6
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.4
SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33 |Schizo... 27 3.4
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.5
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.9
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 250 bits (612), Expect = 2e-67
Identities = 130/254 (51%), Positives = 167/254 (65%), Gaps = 4/254 (1%)
Frame = +3
Query: 93 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 272
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 273 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 452
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 453 RKEAESCDCLQGIP-TDTLARRRHRFRYGHPPHLQNQRRVP*QNNEHIFSCTIT*SVGHS 629
R+EAE+CD LQG T +L G L ++ R + + + ++ + S
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGS---GMGTLLLSKIREE-YPDRMMATFSVAPAPKSS 176
Query: 630 SRTVQRHTFSSSI*LKTQTKP---TVSTTRLSTISASAR*NYPHPTYGDLNHLVSLTISG 800
V+ + + S+ + + LS+I A+ P+Y DLNHLVS ++G
Sbjct: 177 DTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTL-KIKSPSYDDLNHLVSAVMAG 235
Query: 801 VTTCLXFPXQXNXD 842
VTT FP + N D
Sbjct: 236 VTTSFRFPGELNSD 249
Score = 69.3 bits (162), Expect = 6e-13
Identities = 34/62 (54%), Positives = 39/62 (62%)
Frame = +1
Query: 478 ASRGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLS 657
A +GFQ KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188
Query: 658 VH 663
+H
Sbjct: 189 MH 190
Score = 44.0 bits (99), Expect = 3e-05
Identities = 19/29 (65%), Positives = 23/29 (79%)
Frame = +2
Query: 668 LVENTDETYCIDNEALYDICFRTLKLSTP 754
LVEN+DET+CIDNEAL I TLK+ +P
Sbjct: 192 LVENSDETFCIDNEALSSIFANTLKIKSP 220
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 135 bits (326), Expect = 9e-33
Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%)
Frame = +3
Query: 93 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 266
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 267 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 446
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 447 VVRKEAESCDCLQG 488
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 39.1 bits (87), Expect = 8e-04
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +1
Query: 553 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVH 663
++ EY + +SV P+P+VS +VVEPYN+ L+ H
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTH 192
Score = 31.5 bits (68), Expect = 0.16
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +2
Query: 683 DETYCIDNEALYDICFRTLKLSTP 754
D T+ +DNE+ YDIC R L + P
Sbjct: 199 DCTFMVDNESCYDICRRNLDIERP 222
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 130 bits (314), Expect = 2e-31
Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%)
Frame = +3
Query: 93 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 254
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 255 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 434
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 435 SVLDVVRKEAESCDCLQG 488
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 38.7 bits (86), Expect = 0.001
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +1
Query: 553 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVH 663
++ EY + +SV P+P+VS +VVEPYN+ L+ H
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTH 196
Score = 36.7 bits (81), Expect = 0.004
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = +2
Query: 671 VENTDETYCIDNEALYDICFRTLKLSTP 754
++N+D T+ +DNEA YDIC R L + P
Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIERP 226
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 130 bits (313), Expect = 3e-31
Identities = 82/258 (31%), Positives = 136/258 (52%), Gaps = 9/258 (3%)
Frame = +3
Query: 96 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 275
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 276 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 449
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 450 VRKEAESCDCLQGIPTDTLARRRHRFRYGHPPHLQNQRRVP*QNNEHIFSCTIT*SVGHS 629
+ +EA+ D L+G H G L + N+ + T SV +
Sbjct: 122 IDREADGSDSLEGFSL------LHSIAGGTGSGL-GSFLLERLNDRYPKKIIQTYSVFPN 174
Query: 630 SRTVQR---HTFSSSI*LKTQTKPTVSTTRLSTIS----ASAR*NYPHPTYGDLNHLVSL 788
S++V ++S + LK T S L + A+ R + +PT+ N LVS
Sbjct: 175 SQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVST 234
Query: 789 TISGVTTCLXFPXQXNXD 842
+S TT L +P N D
Sbjct: 235 VMSASTTTLRYPGYMNND 252
Score = 41.9 bits (94), Expect = 1e-04
Identities = 18/37 (48%), Positives = 27/37 (72%), Gaps = 1/37 (2%)
Frame = +1
Query: 553 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSV 660
++ + YP +I+ TYSV P S VSD VV+PYN+ L++
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLAL 192
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.6
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +3
Query: 396 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 494
A+GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.4
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +3
Query: 132 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 233
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPCC162.10 |ppk33||serine/threonine protein kinase Ppk33
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 338
Score = 27.1 bits (57), Expect = 3.4
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3
Query: 618 VGHSSRTVQRHTFSSSI*LKTQTK 689
+GHSS+ +Q H F SI L+T +K
Sbjct: 289 LGHSSKKLQTHPFFCSISLETVSK 312
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.5
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 327 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 428
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 5.9
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 469 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 332
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 7.8
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 348 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 479
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,026,864
Number of Sequences: 5004
Number of extensions: 57782
Number of successful extensions: 184
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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