BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_G23 (852 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 235 4e-63 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 135 9e-33 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 130 2e-31 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 2e-29 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.6 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.4 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.5 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.9 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.8 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 235 bits (576), Expect = 4e-63 Identities = 101/132 (76%), Positives = 117/132 (88%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 268 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 269 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 448 PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 449 RKEAESCDCLQG 484 R+EAE+CD LQG Sbjct: 121 RREAEACDALQG 132 Score = 109 bits (263), Expect = 4e-25 Identities = 50/71 (70%), Positives = 59/71 (83%) Frame = +1 Query: 571 DRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTP 750 DR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P Sbjct: 161 DRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220 Query: 751 TYGDLNHLXVA 783 +Y DLNHL A Sbjct: 221 SYDDLNHLVSA 231 Score = 31.1 bits (67), Expect = 0.21 Identities = 13/20 (65%), Positives = 15/20 (75%) Frame = +2 Query: 791 SGVXTCLRFPGQLNXDLXKL 850 +GV T RFPG+LN DL KL Sbjct: 234 AGVTTSFRFPGELNSDLRKL 253 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 135 bits (326), Expect = 9e-33 Identities = 62/134 (46%), Positives = 85/134 (63%), Gaps = 2/134 (1%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 262 MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 263 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 442 YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 443 VVRKEAESCDCLQG 484 +R+ A++C LQG Sbjct: 121 KIRRIADNCSGLQG 134 Score = 71.7 bits (168), Expect = 1e-13 Identities = 30/62 (48%), Positives = 44/62 (70%) Frame = +1 Query: 589 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLN 768 +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y +LN Sbjct: 169 FSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLN 228 Query: 769 HL 774 L Sbjct: 229 RL 230 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 130 bits (314), Expect = 2e-31 Identities = 59/138 (42%), Positives = 86/138 (62%), Gaps = 6/138 (4%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 250 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 251 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 430 GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 431 SVLDVVRKEAESCDCLQG 484 SVL+ +R+ A++C LQG Sbjct: 121 SVLERIRRMADNCSGLQG 138 Score = 78.6 bits (185), Expect = 1e-15 Identities = 33/62 (53%), Positives = 46/62 (74%) Frame = +1 Query: 589 YSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLN 768 +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY +LN Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLN 232 Query: 769 HL 774 L Sbjct: 233 RL 234 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 124 bits (299), Expect = 2e-29 Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%) Frame = +2 Query: 92 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 271 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 272 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 445 RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 446 VRKEAESCDCLQG 484 + +EA+ D L+G Sbjct: 122 IDREADGSDSLEG 134 Score = 54.8 bits (126), Expect = 1e-08 Identities = 29/68 (42%), Positives = 38/68 (55%), Gaps = 1/68 (1%) Frame = +1 Query: 574 RIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTP 750 +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN AL I L P Sbjct: 164 KIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223 Query: 751 TYGDLNHL 774 T+ N L Sbjct: 224 TFHQQNQL 231 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 2.6 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = +2 Query: 392 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 490 A+GH G ELV + D +RK++E+ L+ P Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 3.4 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 128 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 229 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 4.5 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +2 Query: 323 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 424 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 26.2 bits (55), Expect = 5.9 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -1 Query: 465 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 328 ++A+ RTTS T+ +PS L P P + S R+ CPK Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 7.8 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +2 Query: 344 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 475 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,066,198 Number of Sequences: 5004 Number of extensions: 59041 Number of successful extensions: 184 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 181 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 422462090 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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