Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_G22
         (865 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc...    64   3e-11
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M...    43   5e-05
SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity...    41   2e-04
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po...    39   0.001
SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|ch...    35   0.013
SPBC660.15 |||mRNA cleavage factor complex subunit |Schizosaccha...    32   0.091
SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    30   0.37 
SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|c...    29   1.1  
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy...    28   2.0  
SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces po...    27   2.6  
SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po...    27   2.6  
SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyc...    27   3.4  
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    26   6.0  
SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr...    26   6.0  
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb...    26   6.0  
SPAC6F6.17 |rif1|tap1, tap11, SPAPJ736.01|telomere length regula...    26   7.9  

>SPBC18H10.04c |sce3|tif48|translation initiation factor
           eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 388

 Score = 63.7 bits (148), Expect = 3e-11
 Identities = 42/112 (37%), Positives = 59/112 (52%), Gaps = 5/112 (4%)
 Frame = +2

Query: 158 SHTRAPVVLPSASRAARG---GLAVDDESIPHRPPFIAHISNLPYDVEESAIAELFADPK 328
           S T   V  P  SR   G   G   D   IP  PPF AH+ NL +D+ E+ + + F +  
Sbjct: 59  SSTFESVRSPPESRREGGMGSGYQRDAIPIPSEPPFTAHVGNLSFDLTENDLGDFFGE-G 117

Query: 329 VTNLRLPRE--GDRLKGHGYVDFEDRENLIEALNMPDLTIGGRRVRIEVSTP 478
           VT++RL  +   +R +G GYV+FE  + L  AL +    + GR VRI V+ P
Sbjct: 118 VTSIRLVIDPLTERSRGFGYVEFETADTLSAALALSGEDLMGRPVRITVAEP 169


>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 500

 Score = 43.2 bits (97), Expect = 5e-05
 Identities = 25/80 (31%), Positives = 40/80 (50%), Gaps = 3/80 (3%)
 Frame = +2

Query: 248 PPFIAHISNLPYDVEESAIAELFAD-PKVTNLRLPRE--GDRLKGHGYVDFEDRENLIEA 418
           P     + NL ++  E  ++  F     + ++RLP +    RLKG GYV F D ++  + 
Sbjct: 364 PSDTVFVGNLSFNATEDDLSTAFGGCGDIQSIRLPTDPQSGRLKGFGYVTFSDIDSAKKC 423

Query: 419 LNMPDLTIGGRRVRIEVSTP 478
           + M    I GR  R++ STP
Sbjct: 424 VEMNGHFIAGRPCRLDFSTP 443



 Score = 32.7 bits (71), Expect = 0.069
 Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 4/75 (5%)
 Frame = +2

Query: 266 ISNLPYDVEESAIAELFAD-PKVTNLRLPREGD--RLKGHGYVDFEDRENLIEALNMPDL 436
           +  L ++V++  + + F +   +   R+  +G   R KG+GYVDFE  E    A+     
Sbjct: 267 VGRLSWNVDDQWLGQEFEEYGTIVGARVIMDGQSGRSKGYGYVDFETPEAAKAAVAANGT 326

Query: 437 -TIGGRRVRIEVSTP 478
             I GR V +++S P
Sbjct: 327 KEIDGRMVNLDLSNP 341


>SPBC3B9.11c |ctf1||mRNA cleavage and polyadenylation specificity
           factor complex subunit Ctf1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 363

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 4/74 (5%)
 Frame = +2

Query: 257 IAHISNLPYDVEESAIAELF---ADPKVTNLRLPREGDRLKGHGYVDFEDRENLIEAL-N 424
           +  + N+PYDV E  + E+F      K   L L  E    KG+G+ +F D E    A+  
Sbjct: 8   VVFVGNIPYDVSEQQMTEIFNQVGPVKTFKLVLDPETGSGKGYGFCEFFDSETTAMAVRK 67

Query: 425 MPDLTIGGRRVRIE 466
           + +  +G R++R+E
Sbjct: 68  LNNSELGPRKIRVE 81


>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1014

 Score = 38.7 bits (86), Expect = 0.001
 Identities = 19/73 (26%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
 Frame = +2

Query: 263 HISNLPYDVEESAIAELFAD-PKVTNLRLPREGDRLKGHGYVDFEDRENLIEALNMPDLT 439
           +++N+ + V E  +   F D  +V ++R+P+  ++ KG GYV     ++   AL+     
Sbjct: 760 YVTNIDFKVNEKDVETFFRDYGQVESVRIPKRFNQHKGFGYVVMTTNQDAENALSAAGKQ 819

Query: 440 IGGRRVRIEVSTP 478
           +G R + + +S P
Sbjct: 820 LGNRVLNVVLSKP 832


>SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 219

 Score = 35.1 bits (77), Expect = 0.013
 Identities = 23/94 (24%), Positives = 49/94 (52%), Gaps = 5/94 (5%)
 Frame = +2

Query: 209 GGLAVDDESIPHRPP--FIAHISNLPYDVEESAIAELFAD-PKVTNLRLPREGD-RLKGH 376
           GG   +D ++    P  F   + NL  DV + ++ + F++ P +   ++ R+ + + +G 
Sbjct: 13  GGQVWEDPTLLEWDPNHFRLFVGNLGNDVNDESLYQAFSEYPSLVKTKVVRDREGKTRGF 72

Query: 377 GYVDFEDRENLIEALNMPD-LTIGGRRVRIEVST 475
           G+V F+D +  ++A    +   IG R V++  +T
Sbjct: 73  GFVSFKDSDQFLKAWREKNGKYIGSRPVKLSRAT 106


>SPBC660.15 |||mRNA cleavage factor complex subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 474

 Score = 32.3 bits (70), Expect = 0.091
 Identities = 13/47 (27%), Positives = 28/47 (59%)
 Frame = +2

Query: 338 LRLPREGDRLKGHGYVDFEDRENLIEALNMPDLTIGGRRVRIEVSTP 478
           L + ++  R +G G+V +E+   +   ++ P +TI G+ V ++ +TP
Sbjct: 278 LMMDKDTGRPRGFGFVTYENESAVEATMSQPYITIHGKPVEVKRATP 324


>SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 674

 Score = 30.3 bits (65), Expect = 0.37
 Identities = 24/79 (30%), Positives = 42/79 (53%), Gaps = 4/79 (5%)
 Frame = +2

Query: 242 HRPPFIAHISNLPYDVEESAIAELFADP--KVTNLRLPREGD-RLKGHGYVDFEDRENLI 412
           +RP  I  I NLP+ +++    E       KV  +++P +G  R+ G  +V  +DR+   
Sbjct: 105 NRPRLI--IRNLPWSIKKPQHLEPHFSKFGKVREIKIPTKGGGRMCGFAFVWMKDRKAAE 162

Query: 413 EALNMPDLT-IGGRRVRIE 466
           EA+N  + T I GR + ++
Sbjct: 163 EAMNSLNGTEIDGRPIAVD 181


>SPAC6F6.04c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 489

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 11/18 (61%), Positives = 13/18 (72%)
 Frame = -1

Query: 508 ASTSSHSTVIWSRHLNSY 455
           AS +S ST+ W  HLNSY
Sbjct: 250 ASFASQSTIAWQSHLNSY 267


>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 436

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 14/45 (31%), Positives = 23/45 (51%), Gaps = 2/45 (4%)
 Frame = +3

Query: 489 EWDEVDALIEIGTMTLSVQWVTGAPDPALYPTRPAAL--VLPMTE 617
           ++ ++DA I++    LS+ W    P P L P   +A   V+P  E
Sbjct: 356 QFSDIDAWIQVACPRLSIDWGYAFPAPLLTPYEASAAFNVVPWKE 400


>SPAC343.15 |||tRNA isopentenyltransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 434

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = +2

Query: 293 ESAIAELFADPKVTNLRLPREGDRLKGHGYVD-FEDRENLIEALNM-PDLTIG 445
           +S I   FAD  V   RL +  D++  HG VD  +  ++L E+    PD T G
Sbjct: 201 KSLIFWAFADSLVLMPRLDKRVDKMLSHGLVDEIKSMKSLAESEKFSPDFTRG 253


>SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 833

 Score = 27.5 bits (58), Expect = 2.6
 Identities = 14/54 (25%), Positives = 27/54 (50%), Gaps = 3/54 (5%)
 Frame = +2

Query: 266 ISNLPYDVEESAIAELFAD-PKVTNLRLP--REGDRLKGHGYVDFEDRENLIEA 418
           + NL Y   E  +  LF    ++  + +P  ++ +  KG  Y+DF D ++ + A
Sbjct: 327 LRNLTYSCAEDDLKSLFGPFGQLEQVHMPIDKKTNNPKGFAYIDFHDADDAVRA 380



 Score = 25.8 bits (54), Expect = 7.9
 Identities = 10/35 (28%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
 Frame = +2

Query: 371 GHGYVDFEDRENLIEALN-MPDLTIGGRRVRIEVS 472
           G G+V+F+D+ + + A++ M    + G ++ I++S
Sbjct: 666 GFGFVEFKDKASAVAAMHAMNGFVLDGHKLEIKLS 700


>SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 434

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 15/35 (42%), Positives = 18/35 (51%)
 Frame = +2

Query: 362 RLKGHGYVDFEDRENLIEALNMPDLTIGGRRVRIE 466
           R KG  YVDF + E +  ALN   L I    + IE
Sbjct: 348 RRKGTAYVDFVNHECVQLALNKKTLQINNATLNIE 382


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 12/34 (35%), Positives = 20/34 (58%)
 Frame = +3

Query: 468 CLLQMTVEWDEVDALIEIGTMTLSVQWVTGAPDP 569
           CL  +  +W      ++I T+ LS+Q + GAP+P
Sbjct: 86  CLSTLKKDWSPA---LQIRTVLLSIQALMGAPNP 116


>SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 647

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 23/78 (29%), Positives = 33/78 (42%), Gaps = 13/78 (16%)
 Frame = +2

Query: 173 PVVLPSASRAARGGLAVDDESIPHRPPFIAHISNL---PYDVEESAIAELFADP------ 325
           P+ L S   ++ G       + P RPP +  I N    PY++E S  +    D       
Sbjct: 456 PMRLSSEPPSSNGFSIAHPNNSPLRPPSLQGILNAEDRPYEIEPSRSSFATNDTGSYNNL 515

Query: 326 ----KVTNLRLPREGDRL 367
                VTNL+ P E DR+
Sbjct: 516 ELLSSVTNLKSPNENDRV 533


>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 262

 Score = 26.2 bits (55), Expect = 6.0
 Identities = 23/86 (26%), Positives = 40/86 (46%), Gaps = 1/86 (1%)
 Frame = -2

Query: 321 SANNSAIALSSTS*GRLEMCAINGGRCGIDSSSTAKP-PRAAREAEGSTTGARVCDAYPK 145
           SA++S  + S++S G +   + +       S+ST      +   A  S  G     +Y  
Sbjct: 178 SASSSVSSSSASSSGSIS--SADAKTVSASSNSTISGFSTSTTSASSSAAGNSSSSSYTS 235

Query: 144 FSVSSSAAFAQLLVVAIVVGSAIMLV 67
           +S + S   AQL V A +  +A+ML+
Sbjct: 236 YSGAVSNGVAQLSVAACMGIAALMLI 261


>SPAC6F6.17 |rif1|tap1, tap11, SPAPJ736.01|telomere length regulator
           protein Rif1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1400

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = +3

Query: 453 EYELRCLLQMTVEWDEVDALIEIGTMTLS 539
           EY LR  L++ + WD V  L + GT+ L+
Sbjct: 710 EYHLRFGLKLMLLWDCVSPLSDDGTLVLA 738


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,830,774
Number of Sequences: 5004
Number of extensions: 53083
Number of successful extensions: 170
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)