BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_G19 (842 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 265 5e-72 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 140 2e-34 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 139 4e-34 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 135 6e-33 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.5 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.3 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.4 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 5.8 SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 5.8 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.8 SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 7.7 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 265 bits (650), Expect = 5e-72 Identities = 117/174 (67%), Positives = 137/174 (78%) Frame = +1 Query: 73 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 252 MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 253 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 432 PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 433 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPRPK 594 R+E+E+CD LQGFQ KIREEYPDR+M T+SV P PK Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174 Score = 55.2 bits (127), Expect = 1e-08 Identities = 24/29 (82%), Positives = 27/29 (93%) Frame = +3 Query: 579 SPSPKVSDTVVEPYNATLSVHQLVENXDE 665 +P+PK SDTVVEPYNATLS+HQLVEN DE Sbjct: 170 APAPKSSDTVVEPYNATLSMHQLVENSDE 198 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 140 bits (340), Expect = 2e-34 Identities = 68/179 (37%), Positives = 98/179 (54%), Gaps = 2/179 (1%) Frame = +1 Query: 73 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 246 MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 247 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 426 YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 427 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPRPKYQT 603 +R+ +++C LQGF ++ EY + +SV P P+ T Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVST 179 Score = 33.9 bits (74), Expect = 0.029 Identities = 13/27 (48%), Positives = 20/27 (74%) Frame = +3 Query: 582 PSPKVSDTVVEPYNATLSVHQLVENXD 662 P+P+VS +VVEPYN+ L+ H ++ D Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLAD 199 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 139 bits (337), Expect = 4e-34 Identities = 61/172 (35%), Positives = 104/172 (60%), Gaps = 2/172 (1%) Frame = +1 Query: 76 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 255 REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 256 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 429 RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 430 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP 585 + +E++ D L+GF ++ + YP +I+ TYSV P Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173 Score = 30.3 bits (65), Expect = 0.36 Identities = 13/26 (50%), Positives = 18/26 (69%) Frame = +3 Query: 585 SPKVSDTVVEPYNATLSVHQLVENXD 662 S VSD VV+PYN+ L++ +L N D Sbjct: 175 SQSVSDVVVQPYNSLLALKRLTLNAD 200 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 135 bits (327), Expect = 6e-33 Identities = 66/183 (36%), Positives = 99/183 (54%), Gaps = 6/183 (3%) Frame = +1 Query: 73 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 234 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 235 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 414 GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 415 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPRPK 594 SVL+ +R+ +++C LQGF ++ EY + +SV P P+ Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180 Query: 595 YQT 603 T Sbjct: 181 VST 183 Score = 37.5 bits (83), Expect = 0.002 Identities = 14/27 (51%), Positives = 21/27 (77%) Frame = +3 Query: 582 PSPKVSDTVVEPYNATLSVHQLVENXD 662 P+P+VS +VVEPYN+ L+ H ++N D Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSD 203 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 2.5 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +1 Query: 376 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 465 A+GH G ELV + D +RK+SE+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 3.3 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +1 Query: 112 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 213 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 4.4 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +1 Query: 307 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 408 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 26.2 bits (55), Expect = 5.8 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = +2 Query: 449 LAIAYRASNLHIPSVAAPGPVWAPSSSQRSVK 544 LA+ Y + + +V APG +W+P+ +R K Sbjct: 159 LAVRYGPLGIRV-NVCAPGTIWSPAWDERFKK 189 >SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 26.2 bits (55), Expect = 5.8 Identities = 11/19 (57%), Positives = 15/19 (78%) Frame = +2 Query: 56 KITYTK*GKSFIYRPANVV 112 +I+YT G SFIYRP N++ Sbjct: 64 EISYT--GTSFIYRPGNII 80 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.2 bits (55), Expect = 5.8 Identities = 19/54 (35%), Positives = 27/54 (50%) Frame = +2 Query: 485 PSVAAPGPVWAPSSSQRSVKSTPTES*THTQ*SLAQSIRHCRRTIQCDSLSSPA 646 PS+A+ P P SSQ S S P S + S A SI ++ + ++SS A Sbjct: 14 PSIASSFPTVKPFSSQNSTTSNPELSHIEKE-SNASSIIISQQPLSPSNISSAA 66 >SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 25.8 bits (54), Expect = 7.7 Identities = 15/44 (34%), Positives = 21/44 (47%) Frame = +3 Query: 507 RYGHPPHLKDP*RVPRQNHEHILSSPSPKVSDTVVEPYNATLSV 638 R PP + P + N +LSSPS SDT P + L++ Sbjct: 291 RLNSPPFITIPQSIKNTNSNFLLSSPS-LFSDTRTRPASYALAL 333 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,840,394 Number of Sequences: 5004 Number of extensions: 53342 Number of successful extensions: 169 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 416455520 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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