Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_G10
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 266 2e-72
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 258 7e-70
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 128 1e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 110 3e-25
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S... 27 4.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 266 bits (653), Expect = 2e-72
Identities = 136/247 (55%), Positives = 168/247 (68%), Gaps = 5/247 (2%)
Frame = +2
Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSV*L--RQEV*TGVRHLPRASGS 634
+IR++AD C+GLQGFL+FH LL+ERL++ + ++ V P+ S S
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 635 HCRRRALQLYPHHPHNP*STLTVLSWSTMKPSMTS---AAXNLDIERPTYTNXNRLIGQI 805
L H +TL + + M + + NLDIERP+Y N NRLI Q+
Sbjct: 181 VVEPYNSVLTTH------ATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQV 234
Query: 806 VSSITAS 826
VSSITAS
Sbjct: 235 VSSITAS 241
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/29 (72%), Positives = 27/29 (93%)
Frame = +3
Query: 579 DYGKKSKLEFAIYPAPQVPTAVVEPYNSI 665
+Y KKSKL+F++YPAPQV T+VVEPYNS+
Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSV 188
Score = 37.9 bits (84), Expect = 0.002
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = +1
Query: 694 SDCAFMVDNEAIYDICRR 747
+DC FMVDNE+ YDICRR
Sbjct: 198 ADCTFMVDNESCYDICRR 215
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 258 bits (632), Expect = 7e-70
Identities = 138/250 (55%), Positives = 165/250 (66%), Gaps = 8/250 (3%)
Frame = +2
Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 266 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 445
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 446 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSV*L--RQEV*TGVRHLP 619
D VL+RIR++AD C+GLQGFL+FH LL+ERL++ + + V P
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 620 RASGSHCRRRALQLYPHHP-HNP*STLTVLSWSTMKPSMTSAAXNLDIERPTYTNXNRLI 796
+ S S L H N T V + NLDIERPTY N NRLI
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMV----DNEACYDICRRNLDIERPTYENLNRLI 235
Query: 797 GQIVSSITAS 826
Q+VSSITAS
Sbjct: 236 AQVVSSITAS 245
Score = 54.0 bits (124), Expect = 3e-08
Identities = 21/29 (72%), Positives = 27/29 (93%)
Frame = +3
Query: 579 DYGKKSKLEFAIYPAPQVPTAVVEPYNSI 665
+YGKKS L+F++YPAPQV T+VVEPYNS+
Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSV 192
Score = 40.7 bits (91), Expect = 3e-04
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +1
Query: 691 HSDCAFMVDNEAIYDICRR 747
+SDC FMVDNEA YDICRR
Sbjct: 201 NSDCTFMVDNEACYDICRR 219
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 128 bits (308), Expect = 1e-30
Identities = 78/245 (31%), Positives = 117/245 (47%), Gaps = 3/245 (1%)
Frame = +2
Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSV*L--RQEV*TGVRHLPRASGS 634
+R+ A+ C LQGF + H LL+ ++ R V P++S +
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 635 HCRRRALQLYPHH-PHNP*STLTVLSWSTMKPSMTSAAXNLDIERPTYTNXNRLIGQIVS 811
L H N T + + + A L I+ P+Y + N L+ +++
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCI----DNEALSSIFANTLKIKSPSYDDLNHLVSAVMA 234
Query: 812 SITAS 826
+T S
Sbjct: 235 GVTTS 239
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 579 DYGKKSKLEFAIYPAPQVPTAVVEPYNS 662
+Y + F++ PAP+ VVEPYN+
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNA 185
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 110 bits (264), Expect = 3e-25
Identities = 58/159 (36%), Positives = 90/159 (56%), Gaps = 2/159 (1%)
Frame = +2
Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 283
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 284 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 458 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 574
D I + AD L+GF + H L+ERL+
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158
>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 26.6 bits (56), Expect = 4.4
Identities = 10/31 (32%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +3
Query: 576 FDYGKKSKLEFAIYPAPQVPTAVVE-PYNSI 665
+ Y + K+E YP ++P V + PYN +
Sbjct: 108 YTYNSRWKIEIPSYPVREIPYVVADTPYNKL 138
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.8
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 180 STASSLMARCPQTRPSGVETILSTLSSARPELAST 284
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,362,913
Number of Sequences: 5004
Number of extensions: 68980
Number of successful extensions: 189
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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