BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_G09
(870 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 230 1e-60
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 224 7e-59
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 211 5e-55
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 103 1e-22
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 102 4e-22
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 97 2e-20
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 1.0
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 1.7
At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705... 30 2.3
At3g31540.1 68416.m04025 hypothetical protein 29 3.1
At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like fami... 29 5.3
At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5)... 29 5.3
At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421... 28 7.1
At2g36350.1 68415.m04461 protein kinase, putative similar to pro... 28 7.1
At1g26370.1 68414.m03217 RNA helicase, putative similar to SP|Q1... 28 7.1
At1g75860.1 68414.m08811 expressed protein 28 9.3
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 230 bits (562), Expect = 1e-60
Identities = 107/193 (55%), Positives = 135/193 (69%), Gaps = 2/193 (1%)
Frame = +1
Query: 124 FEXXFPDXSWXSNWVYSE--HPGKXFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 297
FE F D W + WV SE G++K TAG + D +DKG++TSED RFYA+S +
Sbjct: 26 FEERFDD-GWENRWVKSEWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRFYAISAE 83
Query: 298 FKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPG 477
F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK G+TPY IMFGPDICG
Sbjct: 84 FPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICGYS 143
Query: 478 TKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLKADW 657
TKKVH I +Y NHLIKKD+ C+ D TH+YT I++PD TY +LIDN + ++G L +DW
Sbjct: 144 TKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDATYSILIDNVEKQTGSLYSDW 203
Query: 658 DFLPPKKIKDPEA 696
D LPPKKIKDP A
Sbjct: 204 DLLPPKKIKDPSA 216
Score = 33.5 bits (73), Expect = 0.19
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +2
Query: 734 DPEXKKPEDWDKPEHIPXP 790
DP KKPEDWD+ E+I P
Sbjct: 213 DPSAKKPEDWDEQEYISDP 231
Score = 27.9 bits (59), Expect = 9.3
Identities = 16/54 (29%), Positives = 24/54 (44%), Gaps = 4/54 (7%)
Frame = +3
Query: 687 P*SXKPEDWDDKPTXQTPXTKSL----RIGTNLNTFXTQMPQT*XWDXXMDGKW 836
P + KPEDWD++ P K I + ++ P+ WD DG+W
Sbjct: 214 PSAKKPEDWDEQEYISDPEDKKPDGYDDIPKEIPDTDSKKPE--DWDDEEDGEW 265
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 224 bits (547), Expect = 7e-59
Identities = 103/193 (53%), Positives = 133/193 (68%), Gaps = 2/193 (1%)
Frame = +1
Query: 124 FEXXFPDXSWXSNWVYSE--HPGKXFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 297
FE F D W WV S+ G++K TAG + D +DKG++TSED RFYA+S +
Sbjct: 26 FEEKFED-GWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRFYAISAE 83
Query: 298 FKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPG 477
F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q G+TPY IMFGPDICG
Sbjct: 84 FPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGPDICGYS 143
Query: 478 TKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLKADW 657
TKKVH I +Y G NHLIKK++ C+ D TH+YT +++PD TY +LIDN + ++G L +DW
Sbjct: 144 TKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSILIDNVEKQTGSLYSDW 203
Query: 658 DFLPPKKIKDPEA 696
D LP KKIKDP A
Sbjct: 204 DLLPAKKIKDPSA 216
Score = 35.9 bits (79), Expect = 0.035
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +2
Query: 734 DPEXKKPEDWDKPEHIPXP 790
DP KKPEDWD E+IP P
Sbjct: 213 DPSAKKPEDWDDKEYIPDP 231
Score = 30.7 bits (66), Expect = 1.3
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 4/60 (6%)
Frame = +3
Query: 669 AQENQGP*SXKPEDWDDKPTXQTP-XTKSL---RIGTNLNTFXTQMPQT*XWDXXMDGKW 836
A++ + P + KPEDWDDK P TK I + + P+ WD DG+W
Sbjct: 208 AKKIKDPSAKKPEDWDDKEYIPDPEDTKPAGYDDIPKEIPDTDAKKPE--DWDDEEDGEW 265
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 211 bits (515), Expect = 5e-55
Identities = 99/191 (51%), Positives = 133/191 (69%), Gaps = 3/191 (1%)
Frame = +1
Query: 121 FFEXXFPDXSWXSNWVYSE---HPGKXFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALS 291
F E F + W S WV S+ + GK G FK TAGK+ DP D+KG++T DA+ YA+S
Sbjct: 31 FLEEHF-EGGWKSRWVLSDWKRNEGKA-GTFKHTAGKWPGDP-DNKGIQTYNDAKHYAIS 87
Query: 292 RKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICG 471
K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY +MFGPDICG
Sbjct: 88 AKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDICG 147
Query: 472 PGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLKA 651
TKK+HVI SY+G+N+ IKKD++C+ D H YT I++PD +Y VL+DN++ E G +
Sbjct: 148 TQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSVLVDNKEREFGSMYT 207
Query: 652 DWDFLPPKKIK 684
DWD LPP+KIK
Sbjct: 208 DWDILPPRKIK 218
Score = 29.9 bits (64), Expect = 2.3
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +2
Query: 746 KKPEDWDKPEHIPXPN 793
KKPEDWD E+I PN
Sbjct: 223 KKPEDWDDREYIDDPN 238
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 103 bits (248), Expect = 1e-22
Identities = 53/113 (46%), Positives = 73/113 (64%), Gaps = 3/113 (2%)
Frame = +1
Query: 121 FFEXXFPDXSWXSNWVYSE---HPGKXFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALS 291
F E F + W S WV S+ + GK G FK TAGK+ DP D+KG++T DA+ YA+S
Sbjct: 31 FLEEHF-EGGWKSRWVLSDWKRNEGKA-GTFKHTAGKWPGDP-DNKGIQTYNDAKHYAIS 87
Query: 292 RKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIM 450
K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY ++
Sbjct: 88 AKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVL 140
Score = 44.4 bits (100), Expect = 1e-04
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = +1
Query: 592 DNTYEVLIDNEKVESGDLKADWDFLPPKKIK 684
D Y VL+DN++ E G + DWD LPP+KIK
Sbjct: 134 DTPYSVLVDNKEREFGSMYTDWDILPPRKIK 164
Score = 29.9 bits (64), Expect = 2.3
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +2
Query: 746 KKPEDWDKPEHIPXPN 793
KKPEDWD E+I PN
Sbjct: 169 KKPEDWDDREYIDDPN 184
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 102 bits (244), Expect = 4e-22
Identities = 60/169 (35%), Positives = 91/169 (53%), Gaps = 14/169 (8%)
Frame = +1
Query: 229 SDPEDDKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVF--- 396
S+ +D GL SE AR Y + ++ +P + + +V+Q+ V+ ++ ++CGG YLK
Sbjct: 60 SEGHEDYGLLVSEKARKYGIVKELDEPLNLKEGTVVLQYEVRFQEGLECGGAYLKYLRPQ 119
Query: 397 DCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKN------HLIKKDIRCKDDV 558
+ + E+PY IMFGPD CG GT KVH I +K H +K D
Sbjct: 120 EAGWTPQGFDSESPYSIMFGPDKCG-GTNKVHFILKHKNPKSGEYVEHHLKFPPSVPYDK 178
Query: 559 YTHLYTLIVKPDNTYEVLIDNEKVESGDLKADWDF----LPPKKIKDPE 693
+H+YT I+KPDN +L+D E+ + +L + DF +P K I DPE
Sbjct: 179 LSHVYTAILKPDNEVRILVDGEEKKKANLLSGEDFEPALIPAKTIPDPE 227
Score = 38.7 bits (86), Expect = 0.005
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = +2
Query: 734 DPEXKKPEDWDKPEHIPXPNA 796
DPE KKPEDWD+ IP PNA
Sbjct: 225 DPEDKKPEDWDERAKIPDPNA 245
Score = 27.9 bits (59), Expect = 9.3
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2
Query: 734 DPEXKKPEDWDKPE 775
DPE KPEDWD E
Sbjct: 280 DPEATKPEDWDDEE 293
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 96.7 bits (230), Expect = 2e-20
Identities = 60/172 (34%), Positives = 92/172 (53%), Gaps = 17/172 (9%)
Frame = +1
Query: 229 SDPEDDKGLKTSEDARFYALSRKF---KPFS-NEGKPLVVQFTVKHEQDIDCGGGYLKVF 396
S+ DD GL SE A+ Y + ++ +P + NEG +V+Q+ + ++ ++CGG YLK
Sbjct: 60 SEGHDDYGLLVSEKAKKYGIVKELDVDEPLNLNEGT-VVLQYEARFQEGLECGGAYLKYL 118
Query: 397 ---DCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKN------HLIKKDIRCK 549
+ + ++PY IMFGPD CG T KVH I +K H +K
Sbjct: 119 RPQEAGWVPQGFDNDSPYSIMFGPDKCG-ATNKVHFILKHKNPKSGEFVEHHLKFPPSVP 177
Query: 550 DDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLKADWDFLPP----KKIKDPE 693
D+ +H+YT ++K DN +L+D E+ + G+L + DF PP K I DPE
Sbjct: 178 FDMLSHVYTAVLKSDNEVRILVDGEEKKKGNLLSAEDFEPPLIPSKTIPDPE 229
Score = 38.7 bits (86), Expect = 0.005
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = +2
Query: 734 DPEXKKPEDWDKPEHIPXPNA 796
DPE KKPEDWD+ IP PNA
Sbjct: 227 DPEDKKPEDWDERAKIPDPNA 247
Score = 28.3 bits (60), Expect = 7.1
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2
Query: 734 DPEXKKPEDWDKPE 775
DPE KPEDWD E
Sbjct: 282 DPEASKPEDWDDEE 295
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 1.0
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +1
Query: 334 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 447
++F KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 1.7
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 334 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 444
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705:
Eukaryotic protein of unknown function (DUF829)
Length = 420
Score = 29.9 bits (64), Expect = 2.3
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -2
Query: 422 MSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNL 294
+ F S L K +P + MS V+W KG+ S L+GL L
Sbjct: 147 LDFTSDLNVKFALHPTIRRMSGPSRLVSWVAKGISSGLDGLYL 189
>At3g31540.1 68416.m04025 hypothetical protein
Length = 699
Score = 29.5 bits (63), Expect = 3.1
Identities = 17/53 (32%), Positives = 22/53 (41%)
Frame = +1
Query: 514 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLKADWDFLPP 672
KN I + CKDD YT + + D E L+ + G L FL P
Sbjct: 252 KNMKIIEGFPCKDDAYTDYFFFVALEDAVLEDLVGKVLTKWGILDRPIRFLEP 304
>At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like family
protein contains Pfam profile PF04091: Exocyst complex
subunit Sec15-like
Length = 789
Score = 28.7 bits (61), Expect = 5.3
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = +1
Query: 514 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLKAD 654
KN KK+ +D TH IV D VL+D E+++S DL +D
Sbjct: 53 KNVARKKEAEIEDLCKTHYEEFIVAVDELRGVLVDAEELKS-DLASD 98
>At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5) /
gibberellin-responsive protein 5 identical to GASA5
[Arabidopsis thaliana] GI:1289320
Length = 97
Score = 28.7 bits (61), Expect = 5.3
Identities = 15/37 (40%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -2
Query: 428 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 327
PCM FC K L PP +C C NW TK
Sbjct: 55 PCMFFCLKCCKKCLCVPPGTFGNKQTCPCYN-NWKTK 90
>At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421:
Protein of unknown function (DUF751)
Length = 185
Score = 28.3 bits (60), Expect = 7.1
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
Frame = +2
Query: 266 KMRGSMLSP---VSSNRSVMRVNPWSSSSLSNMNKTLTVEADTSRSLTANWSRRTCTER 433
KMR S L P +S+RS+ + SSS+ N L R LT SR + ER
Sbjct: 8 KMRLSCLQPHLHFTSSRSIAAALAFPSSSIRNRTSCLVRCGSARRRLTTKSSRNSKLER 66
>At2g36350.1 68415.m04461 protein kinase, putative similar to
protein kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 949
Score = 28.3 bits (60), Expect = 7.1
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = +2
Query: 314 MRVNPWSSS----SLSNMNKTLTVEADTSRSLTANWSRRTCTERLHMRL 448
+R NP SS SLS+ N + TS S +N SR +C + HM +
Sbjct: 489 IRANPTSSEKFDFSLSSKNSLGDYSSSTSMSEESNLSRFSCGNKPHMSM 537
>At1g26370.1 68414.m03217 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 717
Score = 28.3 bits (60), Expect = 7.1
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = -1
Query: 678 FLGRKEVPIGL*V--ARFNFLIVNEDFIGVVGFHNQSVQMCVDIIFAADIFFDEVVLTLV 505
+ GRK P+ L + A + + +E F G H Q Q VDI++ D V TLV
Sbjct: 221 YQGRKLSPLKLIIMSASLDARVFSEYFGGAKAVHVQGRQFPVDILYTVHPESDYVDATLV 280
Query: 504 T 502
T
Sbjct: 281 T 281
>At1g75860.1 68414.m08811 expressed protein
Length = 283
Score = 27.9 bits (59), Expect = 9.3
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 661 FLPPKKIKDPEAXNQKTGMTSPLXRPR 741
FLP KK+ D +K+G+T L P+
Sbjct: 63 FLPSKKVSDESDSLEKSGLTDELEEPQ 89
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,324,726
Number of Sequences: 28952
Number of extensions: 395922
Number of successful extensions: 1068
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 993
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1058
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2038669600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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