Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_F10
         (852 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   234   1e-62
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   225   5e-60
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   122   9e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   105   6e-24
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.8  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  234 bits (572), Expect = 1e-62
 Identities = 100/139 (71%), Positives = 118/139 (84%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGN 280
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG G 
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 461 RIRKLADQCTGLQGFLIFH 517
           +IR++AD C+GLQGFL+FH
Sbjct: 121 KIRRIADNCSGLQGFLVFH 139



 Score =  138 bits (335), Expect = 7e-34
 Identities = 62/83 (74%), Positives = 72/83 (86%)
 Frame = +3

Query: 579 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNLDI 758
           +Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE  YDICRRNLDI
Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDI 219

Query: 759 ERPTYXXLNRLIGQIVSSXTXSL 827
           ERP+Y  LNRLI Q+VSS T SL
Sbjct: 220 ERPSYENLNRLIAQVVSSITASL 242


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  225 bits (551), Expect = 5e-60
 Identities = 101/144 (70%), Positives = 120/144 (83%), Gaps = 5/144 (3%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 266 TGAGNHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 445
           TG G  VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 446 DLVLDRIRKLADQCTGLQGFLIFH 517
           D VL+RIR++AD C+GLQGFL+FH
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFH 143



 Score =  144 bits (349), Expect = 1e-35
 Identities = 64/83 (77%), Positives = 73/83 (87%)
 Frame = +3

Query: 579 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNLDI 758
           +YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNE  YDICRRNLDI
Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDI 223

Query: 759 ERPTYXXLNRLIGQIVSSXTXSL 827
           ERPTY  LNRLI Q+VSS T SL
Sbjct: 224 ERPTYENLNRLIAQVVSSITASL 246


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  122 bits (293), Expect = 9e-29
 Identities = 58/139 (41%), Positives = 80/139 (57%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGN 280
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   G 
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 461 RIRKLADQCTGLQGFLIFH 517
            +R+ A+ C  LQGF + H
Sbjct: 119 VVRREAEACDALQGFQLTH 137



 Score = 68.9 bits (161), Expect = 8e-13
 Identities = 31/82 (37%), Positives = 48/82 (58%)
 Frame = +3

Query: 579 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNLDI 758
           +Y  +    F++ PAP+ S  VVEPYN+ L+ H  +E+SD  F +DNE +  I    L I
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217

Query: 759 ERPTYXXLNRLIGQIVSSXTXS 824
           + P+Y  LN L+  +++  T S
Sbjct: 218 KSPSYDDLNHLVSAVMAGVTTS 239


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  105 bits (253), Expect = 6e-24
 Identities = 54/140 (38%), Positives = 83/140 (59%), Gaps = 2/140 (1%)
 Frame = +2

Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGNH 283
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++    +
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 284 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 458 DRIRKLADQCTGLQGFLIFH 517
           D I + AD    L+GF + H
Sbjct: 120 DMIDREADGSDSLEGFSLLH 139



 Score = 51.2 bits (117), Expect = 2e-07
 Identities = 26/83 (31%), Positives = 45/83 (54%), Gaps = 1/83 (1%)
 Frame = +3

Query: 582 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEXIYDICRRNLDI 758
           Y KK    ++++P  Q VS  VV+PYNS+L       ++D   ++DN  +  I    L  
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220

Query: 759 ERPTYXXLNRLIGQIVSSXTXSL 827
           + PT+   N+L+  ++S+ T +L
Sbjct: 221 QNPTFHQQNQLVSTVMSASTTTL 243


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,232,949
Number of Sequences: 5004
Number of extensions: 65771
Number of successful extensions: 189
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)