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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_F09
         (839 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   303   1e-83
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   164   1e-41
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   157   2e-39
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   155   1e-38
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        28   1.4  
SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po...    26   5.8  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  303 bits (745), Expect = 1e-83
 Identities = 139/211 (65%), Positives = 163/211 (77%)
 Frame = +2

Query: 47  MXXXVHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLERISVYYNEASVATAE 226
           M   VH+QAGQCGNQ+GA FW  I++EHG+D  G+Y GTS+ Q ER++VY+NEA+     
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAA----- 55

Query: 227 SGGKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 406
            GGKYVPRA+L+DLEPGTMDAV+SG +G LFRPDN ++GQSGAGN WAKGHYTEGAEL D
Sbjct: 56  -GGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELAD 114

Query: 407 AVLDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 586
           AVLDVVR+E E CD LQGFQ                   KIREEYPDR+M T+SV P+PK
Sbjct: 115 AVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174

Query: 587 VSDTVVEPYNAVLSIHQLVENTDETYCIDNE 679
            SDTVVEPYNA LS+HQLVEN+DET+CIDNE
Sbjct: 175 SSDTVVEPYNATLSMHQLVENSDETFCIDNE 205



 Score = 30.3 bits (65), Expect = 0.36
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = +3

Query: 705 TLKVPNPTYGDLNHLXSLTCXG 770
           TLK+ +P+Y DLNHL S    G
Sbjct: 214 TLKIKSPSYDDLNHLVSAVMAG 235


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  164 bits (399), Expect = 1e-41
 Identities = 83/213 (38%), Positives = 123/213 (57%), Gaps = 2/213 (0%)
 Frame = +2

Query: 47  MXXXVHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLERISVYYNEA-SVATA 223
           M   + +  GQ G QIG   WE    EHGI P G    T + ++ + + Y N+      +
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGF--PTENSEVHKNNSYLNDGFGTFFS 58

Query: 224 ESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAEL 400
           E+G GK+VPR+I +DLEP  +D VR+G Y  LF P+  V G+  A NN+A+GHYT G E+
Sbjct: 59  ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118

Query: 401 VDAVLDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 580
           +D+VL+ +R+  +NC  LQGF                    ++  EY  +    +SV P+
Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178

Query: 581 PKVSDTVVEPYNAVLSIHQLVENTDETYCIDNE 679
           P+VS +VVEPYN+VL+ H  ++N+D T+ +DNE
Sbjct: 179 PQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNE 211



 Score = 30.7 bits (66), Expect = 0.27
 Identities = 12/21 (57%), Positives = 14/21 (66%)
 Frame = +3

Query: 687 YDICYRTLKVPNPTYGDLNHL 749
           YDIC R L +  PTY +LN L
Sbjct: 214 YDICRRNLDIERPTYENLNRL 234


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  157 bits (381), Expect = 2e-39
 Identities = 82/213 (38%), Positives = 113/213 (53%), Gaps = 2/213 (0%)
 Frame = +2

Query: 47  MXXXVHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLER--ISVYYNEASVAT 220
           M   + +  GQ G QIG   WE    EHGI P G     +  Q      S +++E     
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQ-- 58

Query: 221 AESGGKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAEL 400
               GKYVPR+I +DLEP  +D VR+G Y  LF P+  + G+  A NN+A+GHYT G EL
Sbjct: 59  ----GKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKEL 114

Query: 401 VDAVLDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 580
           VD V D +R+  +NC  LQGF                    ++  EY  +    +SV P+
Sbjct: 115 VDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174

Query: 581 PKVSDTVVEPYNAVLSIHQLVENTDETYCIDNE 679
           P+VS +VVEPYN+VL+ H  ++  D T+ +DNE
Sbjct: 175 PQVSTSVVEPYNSVLTTHATLDLADCTFMVDNE 207



 Score = 29.1 bits (62), Expect = 0.82
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = +3

Query: 687 YDICYRTLKVPNPTYGDLNHL 749
           YDIC R L +  P+Y +LN L
Sbjct: 210 YDICRRNLDIERPSYENLNRL 230


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  155 bits (375), Expect = 1e-38
 Identities = 75/209 (35%), Positives = 120/209 (57%), Gaps = 3/209 (1%)
 Frame = +2

Query: 59  VHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLERISVYYNEASVATAESGGK 238
           + LQAGQCGNQIG++FW+ +  EHGI P G     +   ++R  V++ ++         +
Sbjct: 6   ITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS------DDTR 59

Query: 239 YVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDAV 412
           Y+PRAIL+DLEP  ++ + S  YG L+ P+N +  ++  GAGNNWA G Y+    + + +
Sbjct: 60  YIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDI 118

Query: 413 LDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKV 589
           +D++ +E +  D L+GF                    ++ + YP +I+ TYSV P S  V
Sbjct: 119 MDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSV 178

Query: 590 SDTVVEPYNAVLSIHQLVENTDETYCIDN 676
           SD VV+PYN++L++ +L  N D    +DN
Sbjct: 179 SDVVVQPYNSLLALKRLTLNADSVVVLDN 207


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 28.3 bits (60), Expect = 1.4
 Identities = 14/30 (46%), Positives = 18/30 (60%)
 Frame = +2

Query: 368 AKGHYTEGAELVDAVLDVVRKECENCDRLQ 457
           A+GH   G ELV A  D +RK+ EN   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 622

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +2

Query: 158 GTSDLQLERISVYYNEASVATAESGGKYVPRAILLDLE 271
           G+SD+ +  +S +  EAS  +  S    VP+ +L D +
Sbjct: 49  GSSDVTMTSVSGHLTEASFPSEYSSWSSVPQDVLFDAQ 86


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,510,968
Number of Sequences: 5004
Number of extensions: 42708
Number of successful extensions: 149
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 414453330
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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