BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_F09 (839 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 303 1e-83 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 164 1e-41 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 157 2e-39 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 155 1e-38 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 28 1.4 SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 26 5.8 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 303 bits (745), Expect = 1e-83 Identities = 139/211 (65%), Positives = 163/211 (77%) Frame = +2 Query: 47 MXXXVHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLERISVYYNEASVATAE 226 M VH+QAGQCGNQ+GA FW I++EHG+D G+Y GTS+ Q ER++VY+NEA+ Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAA----- 55 Query: 227 SGGKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 406 GGKYVPRA+L+DLEPGTMDAV+SG +G LFRPDN ++GQSGAGN WAKGHYTEGAEL D Sbjct: 56 -GGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELAD 114 Query: 407 AVLDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 586 AVLDVVR+E E CD LQGFQ KIREEYPDR+M T+SV P+PK Sbjct: 115 AVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174 Query: 587 VSDTVVEPYNAVLSIHQLVENTDETYCIDNE 679 SDTVVEPYNA LS+HQLVEN+DET+CIDNE Sbjct: 175 SSDTVVEPYNATLSMHQLVENSDETFCIDNE 205 Score = 30.3 bits (65), Expect = 0.36 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +3 Query: 705 TLKVPNPTYGDLNHLXSLTCXG 770 TLK+ +P+Y DLNHL S G Sbjct: 214 TLKIKSPSYDDLNHLVSAVMAG 235 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 164 bits (399), Expect = 1e-41 Identities = 83/213 (38%), Positives = 123/213 (57%), Gaps = 2/213 (0%) Frame = +2 Query: 47 MXXXVHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLERISVYYNEA-SVATA 223 M + + GQ G QIG WE EHGI P G T + ++ + + Y N+ + Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGF--PTENSEVHKNNSYLNDGFGTFFS 58 Query: 224 ESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAEL 400 E+G GK+VPR+I +DLEP +D VR+G Y LF P+ V G+ A NN+A+GHYT G E+ Sbjct: 59 ETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEM 118 Query: 401 VDAVLDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 580 +D+VL+ +R+ +NC LQGF ++ EY + +SV P+ Sbjct: 119 IDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178 Query: 581 PKVSDTVVEPYNAVLSIHQLVENTDETYCIDNE 679 P+VS +VVEPYN+VL+ H ++N+D T+ +DNE Sbjct: 179 PQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNE 211 Score = 30.7 bits (66), Expect = 0.27 Identities = 12/21 (57%), Positives = 14/21 (66%) Frame = +3 Query: 687 YDICYRTLKVPNPTYGDLNHL 749 YDIC R L + PTY +LN L Sbjct: 214 YDICRRNLDIERPTYENLNRL 234 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 157 bits (381), Expect = 2e-39 Identities = 82/213 (38%), Positives = 113/213 (53%), Gaps = 2/213 (0%) Frame = +2 Query: 47 MXXXVHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLER--ISVYYNEASVAT 220 M + + GQ G QIG WE EHGI P G + Q S +++E Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQ-- 58 Query: 221 AESGGKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAEL 400 GKYVPR+I +DLEP +D VR+G Y LF P+ + G+ A NN+A+GHYT G EL Sbjct: 59 ----GKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKEL 114 Query: 401 VDAVLDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 580 VD V D +R+ +NC LQGF ++ EY + +SV P+ Sbjct: 115 VDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174 Query: 581 PKVSDTVVEPYNAVLSIHQLVENTDETYCIDNE 679 P+VS +VVEPYN+VL+ H ++ D T+ +DNE Sbjct: 175 PQVSTSVVEPYNSVLTTHATLDLADCTFMVDNE 207 Score = 29.1 bits (62), Expect = 0.82 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +3 Query: 687 YDICYRTLKVPNPTYGDLNHL 749 YDIC R L + P+Y +LN L Sbjct: 210 YDICRRNLDIERPSYENLNRL 230 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 155 bits (375), Expect = 1e-38 Identities = 75/209 (35%), Positives = 120/209 (57%), Gaps = 3/209 (1%) Frame = +2 Query: 59 VHLQAGQCGNQIGAKFWEXISEEHGIDPTGVYRGTSDLQLERISVYYNEASVATAESGGK 238 + LQAGQCGNQIG++FW+ + EHGI P G + ++R V++ ++ + Sbjct: 6 ITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS------DDTR 59 Query: 239 YVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDAV 412 Y+PRAIL+DLEP ++ + S YG L+ P+N + ++ GAGNNWA G Y+ + + + Sbjct: 60 YIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDI 118 Query: 413 LDVVRKECENCDRLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKV 589 +D++ +E + D L+GF ++ + YP +I+ TYSV P S V Sbjct: 119 MDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSV 178 Query: 590 SDTVVEPYNAVLSIHQLVENTDETYCIDN 676 SD VV+PYN++L++ +L N D +DN Sbjct: 179 SDVVVQPYNSLLALKRLTLNADSVVVLDN 207 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 28.3 bits (60), Expect = 1.4 Identities = 14/30 (46%), Positives = 18/30 (60%) Frame = +2 Query: 368 AKGHYTEGAELVDAVLDVVRKECENCDRLQ 457 A+GH G ELV A D +RK+ EN L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 26.2 bits (55), Expect = 5.8 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +2 Query: 158 GTSDLQLERISVYYNEASVATAESGGKYVPRAILLDLE 271 G+SD+ + +S + EAS + S VP+ +L D + Sbjct: 49 GSSDVTMTSVSGHLTEASFPSEYSSWSSVPQDVLFDAQ 86 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,510,968 Number of Sequences: 5004 Number of extensions: 42708 Number of successful extensions: 149 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 414453330 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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