Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_E24
         (847 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   241   7e-65
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   233   2e-62
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   124   1e-29
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   110   3e-25
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.7  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.7  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  241 bits (591), Expect = 7e-65
 Identities = 106/159 (66%), Positives = 127/159 (79%)
 Frame = +3

Query: 96  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 275
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 276 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 455
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 456 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSV 572
           +IR++AD C+GLQGFL+FH            LL+ERL++
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAM 159



 Score =  125 bits (302), Expect = 7e-30
 Identities = 54/72 (75%), Positives = 64/72 (88%)
 Frame = +1

Query: 574 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLERSDCAFMVDNEAIYDICRRNLDI 753
           +Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDI
Sbjct: 160 EYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDI 219

Query: 754 ERPTYTXXNRLI 789
           ERP+Y   NRLI
Sbjct: 220 ERPSYENLNRLI 231


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  233 bits (570), Expect = 2e-62
 Identities = 107/164 (65%), Positives = 129/164 (78%), Gaps = 5/164 (3%)
 Frame = +3

Query: 96  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 260
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 261 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 440
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 441 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSV 572
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL++
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNM 163



 Score =  131 bits (317), Expect = 1e-31
 Identities = 57/72 (79%), Positives = 64/72 (88%)
 Frame = +1

Query: 574 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLERSDCAFMVDNEAIYDICRRNLDI 753
           +YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL+ SDC FMVDNEA YDICRRNLDI
Sbjct: 164 EYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDI 223

Query: 754 ERPTYTXXNRLI 789
           ERPTY   NRLI
Sbjct: 224 ERPTYENLNRLI 235


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  124 bits (300), Expect = 1e-29
 Identities = 61/157 (38%), Positives = 86/157 (54%)
 Frame = +3

Query: 96  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 275
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 276 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 455
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 456 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERL 566
            +R+ A+ C  LQGF + H            LL+ ++
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKI 155



 Score = 64.1 bits (149), Expect = 2e-11
 Identities = 29/72 (40%), Positives = 42/72 (58%)
 Frame = +1

Query: 574 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLERSDCAFMVDNEAIYDICRRNLDI 753
           +Y  +    F++ PAP+ S  VVEPYN+ L+ H  +E SD  F +DNEA+  I    L I
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKI 217

Query: 754 ERPTYTXXNRLI 789
           + P+Y   N L+
Sbjct: 218 KSPSYDDLNHLV 229


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  110 bits (264), Expect = 3e-25
 Identities = 58/159 (36%), Positives = 90/159 (56%), Gaps = 2/159 (1%)
 Frame = +3

Query: 99  RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 278
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 279 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 452
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 453 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 569
           D I + AD    L+GF + H             L+ERL+
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 25/74 (33%), Positives = 39/74 (52%), Gaps = 1/74 (1%)
 Frame = +1

Query: 577 YGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLERSDCAFMVDNEAIYDICRRNLDI 753
           Y KK    ++++P  Q VS  VV+PYNS+L        +D   ++DN A+  I    L  
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHT 220

Query: 754 ERPTYTXXNRLIGT 795
           + PT+   N+L+ T
Sbjct: 221 QNPTFHQQNQLVST 234


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +1

Query: 175 STASSLMARCPQTRPSGVETILSTLSSARPELAST 279
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 194 WPDAHRQDHRGWRRFFQHFLQR 259
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,272,598
Number of Sequences: 5004
Number of extensions: 67949
Number of successful extensions: 199
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)