Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_E23
         (842 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   295   7e-81
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   289   3e-79
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   148   1e-36
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   129   4e-31
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.7  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.7  
SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein 2|S...    26   7.7  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  295 bits (723), Expect = 7e-81
 Identities = 141/246 (57%), Positives = 171/246 (69%)
 Frame = +3

Query: 105 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 284
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 285 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 464
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 465 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPTA 644
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQV T+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 645 VVEPYNSIXXXXXXXXXXXXCFHGRQ*XHL*HLPP*SRH*APTYTTLNRXIGXIVSSIXA 824
           VVEPYNS+             F                   P+Y  LNR I  +VSSI A
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240

Query: 825 SXKXXG 842
           S +  G
Sbjct: 241 SLRFEG 246



 Score = 47.2 bits (107), Expect = 3e-06
 Identities = 19/24 (79%), Positives = 20/24 (83%)
 Frame = +1

Query: 697 SDCAFMVDNEXIYDICRRNXDIER 768
           +DC FMVDNE  YDICRRN DIER
Sbjct: 198 ADCTFMVDNESCYDICRRNLDIER 221


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  289 bits (709), Expect = 3e-79
 Identities = 143/251 (56%), Positives = 173/251 (68%), Gaps = 5/251 (1%)
 Frame = +3

Query: 105 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 269
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 270 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 449
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 450 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 629
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 630 QVPTAVVEPYNSIXXXXXXXXXXXXCFHGRQ*XHL*HLPP*SRH*APTYTTLNRXIGXIV 809
           QV T+VVEPYNS+             F                   PTY  LNR I  +V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239

Query: 810 SSIXASXKXXG 842
           SSI AS +  G
Sbjct: 240 SSITASLRFAG 250



 Score = 49.2 bits (112), Expect = 7e-07
 Identities = 22/28 (78%), Positives = 23/28 (82%)
 Frame = +1

Query: 694 HSDCAFMVDNEXIYDICRRNXDIERXPT 777
           +SDC FMVDNE  YDICRRN DIER PT
Sbjct: 201 NSDCTFMVDNEACYDICRRNLDIER-PT 227


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  148 bits (358), Expect = 1e-36
 Identities = 72/187 (38%), Positives = 103/187 (55%)
 Frame = +3

Query: 105 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 284
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 285 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 464
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 465 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVPTA 644
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ PAP+    
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 645 VVEPYNS 665
           VVEPYN+
Sbjct: 179 VVEPYNA 185


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  129 bits (312), Expect = 4e-31
 Identities = 70/190 (36%), Positives = 108/190 (56%), Gaps = 3/190 (1%)
 Frame = +3

Query: 108 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 287
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 288 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 461
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 462 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VP 638
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q V 
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 639 TAVVEPYNSI 668
             VV+PYNS+
Sbjct: 180 DVVVQPYNSL 189


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +1

Query: 184 STASSLMARCPQTRPSGVETILSTLSSARPELAST 288
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 203 WPDAHRQDHRGWRRFFQHFLQR 268
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPAC4G9.07 |mug133||S. pombe specific UPF0300 family protein
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 10/29 (34%), Positives = 16/29 (55%), Gaps = 1/29 (3%)
 Frame = +3

Query: 585 YGKKSKLEFAIYPAPQVPTAVVE-PYNSI 668
           Y  + K+E   YP  ++P  V + PYN +
Sbjct: 110 YNSRWKIEIPSYPVREIPYVVADTPYNKL 138


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,253,646
Number of Sequences: 5004
Number of extensions: 66170
Number of successful extensions: 181
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)