Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_D24
(847 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 299 4e-82
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 295 4e-81
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 143 3e-35
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 118 1e-27
SPAC27D7.12c |but1|SPAC27D7.12, mug107|neddylation pathway prote... 28 1.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 1.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 2.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 299 bits (733), Expect = 4e-82
Identities = 136/225 (60%), Positives = 164/225 (72%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPSGFHC 645
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAP
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQ-VST 179
Query: 646 XXXXXXXXXXXXXQPLXHSDCAFMVDNKAIYDICRRYXDIXRPXY 780
L +DC FMVDN++ YDICRR DI RP Y
Sbjct: 180 SVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 295 bits (725), Expect = 4e-81
Identities = 139/230 (60%), Positives = 167/230 (72%), Gaps = 5/230 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 630
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 631 SGFHCXXXXXXXXXXXXXQPLXHSDCAFMVDNKAIYDICRRYXDIXRPXY 780
L +SDC FMVDN+A YDICRR DI RP Y
Sbjct: 180 Q-VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 143 bits (346), Expect = 3e-35
Identities = 77/225 (34%), Positives = 111/225 (49%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPSGFHC 645
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSD- 177
Query: 646 XXXXXXXXXXXXXQPLXHSDCAFMVDNKAIYDICRRYXDIXRPXY 780
Q + +SD F +DN+A+ I I P Y
Sbjct: 178 TVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSY 222
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 118 bits (284), Expect = 1e-27
Identities = 67/214 (31%), Positives = 109/214 (50%), Gaps = 2/214 (0%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPSGFH 642
D I + AD L+GF + H L+ERL+ Y KK ++++P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 643 CXXXXXXXXXXXXXQPLXHSDCAFMVDNKAIYDI 744
+ ++D ++DN A+ I
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213
>SPAC27D7.12c |but1|SPAC27D7.12, mug107|neddylation pathway protein
But1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 243
Score = 28.3 bits (60), Expect = 1.4
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +3
Query: 615 HLPRALRFPLPVVEPYNS 668
HLPRAL+F LPV+ N+
Sbjct: 161 HLPRALQFSLPVISDSNN 178
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 28.3 bits (60), Expect = 1.4
Identities = 24/79 (30%), Positives = 38/79 (48%)
Frame = +2
Query: 515 SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSTPRPQVSTAXRRALQLYPHHPHNPX 694
S+ P V P+ G P P+ TA++ +PS+ P VST R+ PH +P
Sbjct: 1214 STAPPVPTPSAGLPPV-----PVPTAKAPPVPAPSSEAPSVSTP--RSSVPSPHSNASPS 1266
Query: 695 STLTVLSWSTIKPSMTSAA 751
T + S ++ P+ TS +
Sbjct: 1267 PTSS--SMASAAPARTSVS 1283
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.5 bits (58), Expect = 2.5
Identities = 43/187 (22%), Positives = 72/187 (38%)
Frame = +2
Query: 185 STASSLMARCPQTRPSGVETILSTLSSARPELASTYPVXXXXXXXXXXXXXXXXAHTDSC 364
ST SSL + ++PS T ST SSA P S+ + + S
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS----SYLSSSSVVSSSSSPSSSSSS 228
Query: 365 FIQNNLLLVRKMRXXXXXXXXXXLERKS*IWFWTESASSLTSVPVCKDS*SSTPSVEVPA 544
+ ++ L + S T S+SS +S + S SS+ +
Sbjct: 229 TLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSS 288
Query: 545 LGSLPY*WSVSPLTTARSLNWSSPSTPRPQVSTAXRRALQLYPHHPHNPXSTLTVLSWST 724
S S SP +T+ +++ SS S+ ST ++ +P S+ + +S S+
Sbjct: 289 TISSSSSSSSSPTSTSSTIS-SSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSS 347
Query: 725 IKPSMTS 745
PS +S
Sbjct: 348 SSPSSSS 354
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,272,672
Number of Sequences: 5004
Number of extensions: 68137
Number of successful extensions: 280
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 258
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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