Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_D22
         (844 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   233   3e-62
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   132   6e-32
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   127   2e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   122   6e-29
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        27   2.5  
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   3.3  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   4.4  
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c...    26   5.8  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    26   7.7  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  233 bits (569), Expect = 3e-62
 Identities = 100/132 (75%), Positives = 116/132 (87%)
 Frame = +1

Query: 94  MGEIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 273
           M EIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 274 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 453
           PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 454 RKEAESCDCLQG 489
           R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132



 Score =  106 bits (254), Expect = 4e-24
 Identities = 53/92 (57%), Positives = 60/92 (65%)
 Frame = +2

Query: 479 ASRGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLS 658
           A +GFQ                   KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS
Sbjct: 129 ALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLS 188

Query: 659 VHQLVENTDETYCIDNEALYXICFRTLNYPHP 754
           +HQLVEN+DET+CIDNEAL  I   TL    P
Sbjct: 189 MHQLVENSDETFCIDNEALSSIFANTLKIKSP 220



 Score = 50.0 bits (114), Expect = 4e-07
 Identities = 21/35 (60%), Positives = 26/35 (74%)
 Frame = +1

Query: 739 KLSTPTYGDLNHLVSLTMSGVTTCLRXPGXXNADL 843
           K+ +P+Y DLNHLVS  M+GVTT  R PG  N+DL
Sbjct: 216 KIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDL 250


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  132 bits (319), Expect = 6e-32
 Identities = 61/134 (45%), Positives = 84/134 (62%), Gaps = 2/134 (1%)
 Frame = +1

Query: 94  MGEIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 267
           M EI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 268 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 447
           YVPR+I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 448 VVRKEAESCDCLQG 489
            +R+ A++C  LQG
Sbjct: 121 KIRRIADNCSGLQG 134



 Score = 62.5 bits (145), Expect = 7e-11
 Identities = 27/67 (40%), Positives = 42/67 (62%)
 Frame = +2

Query: 554 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYXICFR 733
           ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++  D T+ +DNE+ Y IC R
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215

Query: 734 TLNYPHP 754
            L+   P
Sbjct: 216 NLDIERP 222



 Score = 32.3 bits (70), Expect = 0.089
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +1

Query: 742 LSTPTYGDLNHLVSLTMSGVTTCLRXPGXXNADL 843
           +  P+Y +LN L++  +S +T  LR  G  N DL
Sbjct: 219 IERPSYENLNRLIAQVVSSITASLRFEGSLNVDL 252


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  127 bits (307), Expect = 2e-30
 Identities = 58/138 (42%), Positives = 85/138 (61%), Gaps = 6/138 (4%)
 Frame = +1

Query: 94  MGEIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 255
           M E++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 256 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 435
             GK+VPR+I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 436 SVLDVVRKEAESCDCLQG 489
           SVL+ +R+ A++C  LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138



 Score = 67.3 bits (157), Expect = 3e-12
 Identities = 29/67 (43%), Positives = 44/67 (65%)
 Frame = +2

Query: 554 KIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYXICFR 733
           ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++N+D T+ +DNEA Y IC R
Sbjct: 160 RLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219

Query: 734 TLNYPHP 754
            L+   P
Sbjct: 220 NLDIERP 226



 Score = 33.9 bits (74), Expect = 0.029
 Identities = 14/34 (41%), Positives = 20/34 (58%)
 Frame = +1

Query: 742 LSTPTYGDLNHLVSLTMSGVTTCLRXPGXXNADL 843
           +  PTY +LN L++  +S +T  LR  G  N DL
Sbjct: 223 IERPTYENLNRLIAQVVSSITASLRFAGSLNVDL 256


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  122 bits (294), Expect = 6e-29
 Identities = 51/132 (38%), Positives = 89/132 (67%), Gaps = 2/132 (1%)
 Frame = +1

Query: 100 EIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPR 279
           EI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+PR
Sbjct: 4   EIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPR 63

Query: 280 AILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVV 453
           AIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D++
Sbjct: 64  AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMI 122

Query: 454 RKEAESCDCLQG 489
            +EA+  D L+G
Sbjct: 123 DREADGSDSLEG 134



 Score = 57.2 bits (132), Expect = 3e-09
 Identities = 28/68 (41%), Positives = 41/68 (60%), Gaps = 1/68 (1%)
 Frame = +2

Query: 554 KIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYXICF 730
           ++ + YP +I+ TYSV P S  VSD VV+PYN+ L++ +L  N D    +DN AL  I  
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215

Query: 731 RTLNYPHP 754
             L+  +P
Sbjct: 216 DRLHTQNP 223



 Score = 35.1 bits (77), Expect = 0.013
 Identities = 17/31 (54%), Positives = 18/31 (58%)
 Frame = +1

Query: 751 PTYGDLNHLVSLTMSGVTTCLRXPGXXNADL 843
           PT+   N LVS  MS  TT LR PG  N DL
Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDL 253


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = +1

Query: 397 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 495
           A+GH   G ELV +  D +RK++E+   L+  P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +1

Query: 133 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 234
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +1

Query: 328 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 429
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 807

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = -3

Query: 470 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 333
           ++A+ RTTS T+    +PS       L P P    +  S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 25.8 bits (54), Expect = 7.7
 Identities = 12/44 (27%), Positives = 20/44 (45%)
 Frame = +1

Query: 349  RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 480
            RP   +F     G++   G   +  E  D ++ +  +  ESCDC
Sbjct: 955  RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,144,535
Number of Sequences: 5004
Number of extensions: 61781
Number of successful extensions: 189
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)