Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_D06
         (839 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   283   3e-77
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   275   4e-75
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   140   2e-34
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   131   1e-31
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    27   3.3  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.6  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  283 bits (693), Expect = 3e-77
 Identities = 124/188 (65%), Positives = 151/188 (80%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 281 HVPRAVFVDLEPTVVDEVRTDTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPR+++VDLEP V+D+VRT  YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPXPQVSTA 640
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YP PQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 641 VVXPYESI 664
           VV PY S+
Sbjct: 181 VVEPYNSV 188


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  275 bits (675), Expect = 4e-75
 Identities = 125/193 (64%), Positives = 153/193 (79%), Gaps = 5/193 (2%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 266 TGAGKHVPRAVFVDLEPTVVDEVRTDTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 445
           TG GK VPR+++VDLEP V+D+VRT  Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 446 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPXP 625
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YP P
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 626 QVSTAVVXPYESI 664
           QVST+VV PY S+
Sbjct: 180 QVSTSVVEPYNSV 192


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  140 bits (339), Expect = 2e-34
 Identities = 69/187 (36%), Positives = 100/187 (53%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 281 HVPRAVFVDLEPTVVDEVRTDTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPRAV VDLEP  +D V++  +  LF P+ +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPXPQVSTA 640
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F++ P P+ S  
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 641 VVXPYES 661
           VV PY +
Sbjct: 179 VVEPYNA 185


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  131 bits (316), Expect = 1e-31
 Identities = 71/190 (37%), Positives = 108/190 (56%), Gaps = 3/190 (1%)
 Frame = +2

Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 283
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 284 VPRAVFVDLEPTVVDEVRTDTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457
           +PRA+ +DLEP VV+ + +DTY  L++PE ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 458 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPXPQ-VS 634
           D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179

Query: 635 TAVVXPYESI 664
             VV PY S+
Sbjct: 180 DVVVQPYNSL 189


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 21/74 (28%), Positives = 33/74 (44%)
 Frame = +3

Query: 63  NSTFKHRKKLKSKCVSASLYTSAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETI 242
           +S+      + S  + +S        S S+  +   T+ ST SSL +    ++PS   T 
Sbjct: 135 SSSLLDPSSVSSAILPSSTSVEVSISSSSLSSSDPLTS-STFSSLSSSTSSSQPSVSSTS 193

Query: 243 LSTLSSARPELAST 284
            ST SSA P   S+
Sbjct: 194 SSTFSSAAPTSTSS 207



 Score = 26.2 bits (55), Expect = 5.8
 Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
 Frame = +3

Query: 108 SASLYTSAKPESRSVMPAGSFTAWSTASSL-MARCPQTRPSGVETILSTLSSARPELAST 284
           S+S  +S+   S S  P+ S ++  T+SSL  +  P T  S   T  S  SS+    AS+
Sbjct: 206 SSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASS 265


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,925,503
Number of Sequences: 5004
Number of extensions: 58308
Number of successful extensions: 173
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 414453330
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)