Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_C22
(832 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 361 e-101
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 359 e-100
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 182 7e-47
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 147 2e-36
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 27 4.3
SPBC19G7.15 |nup44||nucleoporin Nup44|Schizosaccharomyces pombe|... 26 5.7
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.5
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 26 7.5
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 10.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 361 bits (888), Expect = e-101
Identities = 162/237 (68%), Positives = 193/237 (81%), Gaps = 1/237 (0%)
Frame = +2
Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 640
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 641 VVEPYNSILTPH-NLEHSDCAFMVDNEAIYDXCRRNLDIERPTYTNLNRLIGQXVXS 808
VVEPYNS+LT H L+ +DC FMVDNE+ YD CRRNLDIERP+Y NLNRLI Q V S
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSS 237
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 359 bits (884), Expect = e-100
Identities = 166/242 (68%), Positives = 196/242 (80%), Gaps = 6/242 (2%)
Frame = +2
Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 266 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 445
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 446 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 625
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 626 QVSTAVVEPYNSILTPH-NLEHSDCAFMVDNEAIYDXCRRNLDIERPTYTNLNRLIGQXV 802
QVST+VVEPYNS+LT H L++SDC FMVDNEA YD CRRNLDIERPTY NLNRLI Q V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239
Query: 803 XS 808
S
Sbjct: 240 SS 241
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 182 bits (442), Expect = 7e-47
Identities = 91/231 (39%), Positives = 130/231 (56%), Gaps = 1/231 (0%)
Frame = +2
Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 461 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 640
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 641 VVEPYNSILTPHNL-EHSDCAFMVDNEAIYDXCRRNLDIERPTYTNLNRLI 790
VVEPYN+ L+ H L E+SD F +DNEA+ L I+ P+Y +LN L+
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLV 229
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 147 bits (356), Expect = 2e-36
Identities = 82/233 (35%), Positives = 129/233 (55%), Gaps = 4/233 (1%)
Frame = +2
Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 283
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 284 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 457
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 458 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 634
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 635 TAVVEPYNSILTPHNLE-HSDCAFMVDNEAIYDXCRRNLDIERPTYTNLNRLI 790
VV+PYNS+L L ++D ++DN A+ L + PT+ N+L+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLV 232
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 26.6 bits (56), Expect = 4.3
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = -1
Query: 709 DHESTVRVLKVVWCEDRVVGLDDGSGNLRRGVD 611
+H + VR++K++ C + D G+GN +D
Sbjct: 470 EHNNPVRIIKLLDCLVLTLQADKGNGNSNNDID 502
>SPBC19G7.15 |nup44||nucleoporin Nup44|Schizosaccharomyces pombe|chr
2|||Manual
Length = 403
Score = 26.2 bits (55), Expect = 5.7
Identities = 12/27 (44%), Positives = 13/27 (48%)
Frame = +2
Query: 623 PQVSTAVVEPYNSILTPHNLEHSDCAF 703
P +V E IL NL H DCAF
Sbjct: 150 PAQPPSVEEQVQKILNAWNLNHPDCAF 176
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.5
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 180 STASSLMARCPQTRPSGVETILSTLSSARPELAST 284
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.5
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 503
Score = 25.8 bits (54), Expect = 7.5
Identities = 15/54 (27%), Positives = 24/54 (44%), Gaps = 2/54 (3%)
Frame = +2
Query: 611 IYPAPQVSTAV--VEPYNSILTPHNLEHSDCAFMVDNEAIYDXCRRNLDIERPT 766
IYP + T + ++ NSI + A + NE CR+ ++RPT
Sbjct: 141 IYPEEMIQTGISSIDGLNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKRPT 194
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 10.0
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 90 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 212
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,295,973
Number of Sequences: 5004
Number of extensions: 69054
Number of successful extensions: 196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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