Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_C20
(844 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 218 7e-58
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 117 2e-27
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 100 3e-22
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 99 4e-22
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 30 0.36
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||... 29 1.1
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 28 1.9
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 28 1.9
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.5
SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|... 27 2.5
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 2.5
SPBC887.12 |||P-type ATPase |Schizosaccharomyces pombe|chr 2|||M... 26 5.8
SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyce... 26 5.8
SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|ch... 26 5.8
SPAC1B3.03c |wis2|cyp5|cyclophilin family peptidyl-prolyl cis-tr... 26 7.7
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 26 7.7
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 218 bits (533), Expect = 7e-58
Identities = 104/156 (66%), Positives = 125/156 (80%)
Frame = +2
Query: 230 GMGSYILEHLSDRFPKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKRLTESADCVMVLDN 409
G+GS++LE L+DR+PKK++QTYSVFPN +SDVVVQPYNSLL LKRLT +AD V+VLDN
Sbjct: 148 GLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDN 207
Query: 410 TALNRIASDRLHIQNPSFAQINTLVSTIMSASTATLRYPSYMNNDLISLVAPLIPTPRLH 589
AL IA+DRLH QNP+F Q N LVST+MSAST TLRYP YMNNDL+S++A LIP+PR H
Sbjct: 208 AALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCH 267
Query: 590 FLMTGYTPLSADHEQTAAPKIRKTTVLXXMXRLLXP 697
FL+T YTP + + + A IRKTTVL M RLL P
Sbjct: 268 FLLTSYTPFT-NQQVEEAKAIRKTTVLDVMRRLLLP 302
Score = 116 bits (278), Expect = 6e-27
Identities = 47/77 (61%), Positives = 64/77 (83%)
Frame = +1
Query: 7 IHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASGYAQGEKLNEEVFDIINREADGSDNLE 186
++ I++ Y LYNPEN+ ++K+GGGAGNNWA+GY+ E++ E++ D+I+READGSD+LE
Sbjct: 74 VNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSHAERIFEDIMDMIDREADGSDSLE 133
Query: 187 GFVLCHSIAGGTGSGXG 237
GF L HSIAGGTGSG G
Sbjct: 134 GFSLLHSIAGGTGSGLG 150
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 117 bits (281), Expect = 2e-27
Identities = 52/131 (39%), Positives = 87/131 (66%)
Frame = +2
Query: 230 GMGSYILEHLSDRFPKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKRLTESADCVMVLDN 409
GMG+ +L + + +P +++ T+SV P + SD VV+PYN+ L++ +L E++D +DN
Sbjct: 146 GMGTLLLSKIREEYPDRMMATFSVAP-APKSSDTVVEPYNATLSMHQLVENSDETFCIDN 204
Query: 410 TALNRIASDRLHIQNPSFAQINTLVSTIMSASTATLRYPSYMNNDLISLVAPLIPTPRLH 589
AL+ I ++ L I++PS+ +N LVS +M+ T + R+P +N+DL L ++P PRLH
Sbjct: 205 EALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLH 264
Query: 590 FLMTGYTPLSA 622
F M G+ PL+A
Sbjct: 265 FFMVGFAPLAA 275
Score = 72.5 bits (170), Expect = 7e-14
Identities = 32/77 (41%), Positives = 48/77 (62%), Gaps = 1/77 (1%)
Frame = +1
Query: 16 IMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNEEVFDIINREADGSDNLEGF 192
+ + + L+ P+N+ + GAGN WA G Y +G +L + V D++ REA+ D L+GF
Sbjct: 76 VKSGKFGNLFRPDNIIYGQ--SGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGF 133
Query: 193 VLCHSIAGGTGSGXGLI 243
L HS+ GGTGSG G +
Sbjct: 134 QLTHSLGGGTGSGMGTL 150
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 100 bits (239), Expect = 3e-22
Identities = 51/145 (35%), Positives = 82/145 (56%), Gaps = 4/145 (2%)
Frame = +2
Query: 230 GMGSYILEHLSDRFPKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKRLTESADCVMVLDN 409
G G+ +LE L+ + KK +SV+P ++S VV+PYNS+LT + ADC ++DN
Sbjct: 148 GFGALLLERLAMEYTKKSKLQFSVYP-APQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206
Query: 410 TALNRIASDRLHIQNPSFAQINTLVSTIMSASTATLRYPSYMNNDLISLVAPLIPTPRLH 589
+ I L I+ PS+ +N L++ ++S+ TA+LR+ +N DL L+P PR+H
Sbjct: 207 ESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIH 266
Query: 590 FLMTGYTPL----SADHEQTAAPKI 652
F + Y P+ A HE + +I
Sbjct: 267 FPLVTYAPIVSAAKAFHESNSVQEI 291
Score = 65.3 bits (152), Expect = 1e-11
Identities = 34/80 (42%), Positives = 46/80 (57%), Gaps = 1/80 (1%)
Frame = +1
Query: 7 IHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNEEVFDIINREADGSDNL 183
I + PY L++PE + K A NN+A G Y G++L +EV D I R AD L
Sbjct: 75 IDQVRTGPYRDLFHPEQLITGKED--ASNNYARGHYTVGKELVDEVTDKIRRIADNCSGL 132
Query: 184 EGFVLCHSIAGGTGSGXGLI 243
+GF++ HS GGTGSG G +
Sbjct: 133 QGFLVFHSFGGGTGSGFGAL 152
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 99 bits (238), Expect = 4e-22
Identities = 50/145 (34%), Positives = 85/145 (58%), Gaps = 4/145 (2%)
Frame = +2
Query: 230 GMGSYILEHLSDRFPKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKRLTESADCVMVLDN 409
G+G+ +LE L+ + KK +SV+P ++S VV+PYNS+LT +++DC ++DN
Sbjct: 152 GLGALLLERLNMEYGKKSNLQFSVYP-APQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210
Query: 410 TALNRIASDRLHIQNPSFAQINTLVSTIMSASTATLRYPSYMNNDLISLVAPLIPTPRLH 589
A I L I+ P++ +N L++ ++S+ TA+LR+ +N DL L+P PR+H
Sbjct: 211 EACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIH 270
Query: 590 FLMTGYTPL----SADHEQTAAPKI 652
F + Y+P+ A HE + +I
Sbjct: 271 FPLVTYSPIVSAAKAFHESNSVQEI 295
Score = 62.1 bits (144), Expect = 1e-10
Identities = 31/80 (38%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
Frame = +1
Query: 7 IHTIMNSPYAKLYNPENVYLSKHGGGAGNNWASG-YAQGEKLNEEVFDIINREADGSDNL 183
I + PY L++PE + K A NN+A G Y G+++ + V + I R AD L
Sbjct: 79 IDQVRTGPYKDLFHPEQMVTGKED--ASNNYARGHYTVGKEMIDSVLERIRRMADNCSGL 136
Query: 184 EGFVLCHSIAGGTGSGXGLI 243
+GF++ HS GGTGSG G +
Sbjct: 137 QGFLVFHSFGGGTGSGLGAL 156
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 30.3 bits (65), Expect = 0.36
Identities = 13/46 (28%), Positives = 30/46 (65%)
Frame = -2
Query: 684 RCIXXSTVVLRIFGAAVCS*SAERGVYPVMRKCSRGVGINGATRLI 547
+ + +++VL +FG+A+C +A+ ++ V+ + +G+G G T L+
Sbjct: 155 KIVLYTSIVLFLFGSAMCG-AAQNMLWLVLCRAVQGIGGGGITSLV 199
>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1260
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 166 DGSDNLEGFVLCHSIAGGTGSG 231
DG + EGF + +IAGG G+G
Sbjct: 1104 DGENGEEGFAMYETIAGGAGAG 1125
>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
Smc6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1140
Score = 27.9 bits (59), Expect = 1.9
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -1
Query: 457 WVLYVEAVTGNAIQCCIIQNHHAVCTLSE 371
W L +E + GN I I+++HH L E
Sbjct: 564 WHLIIERILGNVINGFIVRSHHDQLILKE 592
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/46 (26%), Positives = 29/46 (63%)
Frame = -2
Query: 684 RCIXXSTVVLRIFGAAVCS*SAERGVYPVMRKCSRGVGINGATRLI 547
+ + +++VL +FG+A+C +A+ ++ V+ + +G+G G L+
Sbjct: 155 KIVLYTSIVLFLFGSAMCG-AAQNMLWLVLCRAVQGIGGGGIMSLV 199
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1379
Score = 27.5 bits (58), Expect = 2.5
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = -2
Query: 573 GINGATRLIRSLFMYDGYLKVAVLALIIVDTSVLICANDGFCMWR 439
G+ G I +F+ D YLK L L + D+ L G +WR
Sbjct: 1291 GVLGRDEFIVGMFLIDQYLKGRKLPLKVPDSVWLSSKRMGDMLWR 1335
>SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 539
Score = 27.5 bits (58), Expect = 2.5
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +2
Query: 407 NTALNRIASDRLHIQNPSFAQINTLVSTIMSASTATLRYPSYMNNDLISLVAPLIP 574
NT L ++A+ H+ A I +++ I+ + T Y + D+++LVA +IP
Sbjct: 391 NTKLAKLAT---HVSINLGAAIGVIIAVILFLTRNTWTYIFTSDKDVVALVATIIP 443
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.5 bits (58), Expect = 2.5
Identities = 35/144 (24%), Positives = 60/144 (41%), Gaps = 3/144 (2%)
Frame = +2
Query: 236 GSYILEHLSDRFPKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKRLTE---SADCVMVLD 406
G Y + +SD++ K+ + Y +F +L IS + + L L LT+ D V+
Sbjct: 1385 GGYGVVAVSDKYSKQTLSRYPIFWSLVHISGLPNPDPSDLKLLNHLTDDPVDVDFVLEAK 1444
Query: 407 NTALNRIASDRLHIQNPSFAQINTLVSTIMSASTATLRYPSYMNNDLISLVAPLIPTPRL 586
L + + ++ AQ+ V R+ DLI+ +AP + T
Sbjct: 1445 RKLLKKQTQEWANLDVDPSAQLLVFVG----------RWSHQKGIDLIADLAPKLLTEHN 1494
Query: 587 HFLMTGYTPLSADHEQTAAPKIRK 658
L+T P+ H Q AA K+ +
Sbjct: 1495 VQLIT-IGPVIDLHGQFAAEKLEQ 1517
>SPBC887.12 |||P-type ATPase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1258
Score = 26.2 bits (55), Expect = 5.8
Identities = 15/55 (27%), Positives = 25/55 (45%)
Frame = +2
Query: 272 PKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKRLTESADCVMVLDNTALNRIASD 436
PK L + +S + NL + VVQ + + R T ++VL + + I D
Sbjct: 174 PKFLKEQFSKYANLFFLFTAVVQQIPGITPVNRYTTIGPMLIVLSVSGIKEIMED 228
>SPAC637.13c |||cytoskeletal signaling protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 498
Score = 26.2 bits (55), Expect = 5.8
Identities = 15/33 (45%), Positives = 17/33 (51%)
Frame = +3
Query: 186 GLCFMSLNSRRYRFRXWAHIYWNTCLIGFLRNL 284
GLC NS YRF W H+ N LI F + L
Sbjct: 33 GLC----NSLTYRFEGWRHLVEN--LIAFFKQL 59
>SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1374
Score = 26.2 bits (55), Expect = 5.8
Identities = 16/47 (34%), Positives = 21/47 (44%)
Frame = -2
Query: 540 LFMYDGYLKVAVLALIIVDTSVLICANDGFCMWRRSLAMRFNAVLSR 400
LF+ G+L + L LII D ND + R AVLS+
Sbjct: 127 LFLARGFLSINDLNLIIFDECHHAIGNDAYARIMNDFYHRAKAVLSK 173
>SPAC1B3.03c |wis2|cyp5|cyclophilin family peptidyl-prolyl cis-trans
isomerase Wis2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 25.8 bits (54), Expect = 7.7
Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = +2
Query: 410 TALNRIASDRLHIQNPSFAQ--INTLVSTIMSASTATLRYP 526
TA+ +IASD I N FAQ ++T V+ A + YP
Sbjct: 198 TAIFKIASDLKGIANKQFAQQNLDTAVAKWQKALRYLMEYP 238
>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 25.8 bits (54), Expect = 7.7
Identities = 33/129 (25%), Positives = 50/129 (38%), Gaps = 11/129 (8%)
Frame = +2
Query: 212 QEVQVQGMGSYILEHLSDRFPKKLVQTYSVFPNLDEISDVVVQPYNSLLTLKRLTESAD- 388
QE+ +G+ IL F +QT S N+D + VV N + + L S D
Sbjct: 371 QEILKGMLGANILGFQIPEFAYHFLQTCSRLVNIDIRKNGVVSFENRQIDVIALPISIDP 430
Query: 389 -----CVMVLDNTALNRIASDRL---HI--QNPSFAQINTLVSTIMSASTATLRYPSYMN 538
C+ ++ DR HI + I L S ++S +YP Y
Sbjct: 431 GFIDRCLASPPVEHWAKVLQDRFRGKHIILSHDKLDPIRGLRSKLISFERFLQKYPEYRE 490
Query: 539 NDLISLVAP 565
N ++ VAP
Sbjct: 491 NTILLQVAP 499
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,208,292
Number of Sequences: 5004
Number of extensions: 64519
Number of successful extensions: 179
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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