BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_C08 (859 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 251 8e-68 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 142 6e-35 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 141 1e-34 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 120 3e-28 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 80 3e-16 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 76 7e-15 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 70 4e-13 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 52 1e-07 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.12 SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schiz... 27 4.5 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 6.0 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 26 6.0 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 7.9 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 251 bits (615), Expect = 8e-68 Identities = 116/135 (85%), Positives = 120/135 (88%) Frame = +1 Query: 244 PAIRXVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 423 P +MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97 Query: 424 PEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGD 603 PEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQAVLSLYASGRTTGIVLDSGD Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157 Query: 604 GVSHTXPHLRGIRTP 648 GV+HT P G P Sbjct: 158 GVTHTVPIYEGYALP 172 Score = 69.3 bits (162), Expect = 6e-13 Identities = 30/36 (83%), Positives = 34/36 (94%) Frame = +2 Query: 134 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 241 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPS G+ Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGR 37 Score = 30.7 bits (66), Expect = 0.28 Identities = 16/39 (41%), Positives = 17/39 (43%) Frame = +3 Query: 615 HRAPSTRDTHSPHAIXXXXXXXXXXXXYLMKILTERGYS 731 H P PHAI YLMKIL ERGY+ Sbjct: 161 HTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYT 199 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 142 bits (344), Expect = 6e-35 Identities = 66/131 (50%), Positives = 90/131 (68%), Gaps = 1/131 (0%) Frame = +1 Query: 259 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEH 435 VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEH Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106 Query: 436 PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 615 P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166 Query: 616 TXPHLRGIRTP 648 + P G P Sbjct: 167 SVPIYDGFSIP 177 Score = 44.0 bits (99), Expect = 3e-05 Identities = 17/30 (56%), Positives = 23/30 (76%) Frame = +2 Query: 152 LVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 241 + +DNGSG KAGFAGDD P+ +FP+ G+ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGR 41 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 141 bits (341), Expect = 1e-34 Identities = 60/115 (52%), Positives = 88/115 (76%) Frame = +1 Query: 280 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 459 KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109 Query: 460 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTXP 624 +NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H P Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVP 164 Score = 38.3 bits (85), Expect = 0.001 Identities = 17/34 (50%), Positives = 23/34 (67%) Frame = +2 Query: 140 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 241 E A +V+DNG+G K G+A D+ PR FPS G+ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGR 35 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 120 bits (289), Expect = 3e-28 Identities = 59/127 (46%), Positives = 78/127 (61%), Gaps = 9/127 (7%) Frame = +1 Query: 283 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 459 D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132 Query: 460 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSH 615 LNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192 Query: 616 TXPHLRG 636 P G Sbjct: 193 IIPVAEG 199 Score = 38.3 bits (85), Expect = 0.001 Identities = 14/26 (53%), Positives = 21/26 (80%) Frame = +2 Query: 152 LVVDNGSGMCKAGFAGDDAPRAVFPS 229 +++DNG+G K G+AG+DAP VFP+ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPT 33 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 80.2 bits (189), Expect = 3e-16 Identities = 41/127 (32%), Positives = 73/127 (57%), Gaps = 3/127 (2%) Frame = +1 Query: 271 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 447 + + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ + Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120 Query: 448 TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTX 621 TE NP R MT+++FE +N P++ I + S Y + + ++GIVL+ G+ SH Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180 Query: 622 PHLRGIR 642 P L G R Sbjct: 181 PVLNGER 187 Score = 30.7 bits (66), Expect = 0.28 Identities = 12/24 (50%), Positives = 17/24 (70%) Frame = +2 Query: 152 LVVDNGSGMCKAGFAGDDAPRAVF 223 LV+DNGS +AG+ G+ P+ VF Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVF 50 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 75.8 bits (178), Expect = 7e-15 Identities = 35/92 (38%), Positives = 52/92 (56%) Frame = +1 Query: 325 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 504 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 505 ETFNTPAMYVAIQAVLSLYASGRTTGIVLDSG 600 E+ PA Y+A Q + +ASG+ T ++D G Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIG 154 Score = 39.5 bits (88), Expect = 6e-04 Identities = 16/35 (45%), Positives = 25/35 (71%) Frame = +2 Query: 137 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 241 +EV+A+V+D GS + GF+G+D P+ V PS G+ Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGE 43 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 70.1 bits (164), Expect = 4e-13 Identities = 36/134 (26%), Positives = 70/134 (52%), Gaps = 2/134 (1%) Frame = +1 Query: 244 PAIRXVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 417 P+ V + ++ YV DE Q I + +K +GI+ +W+ W +L+ Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96 Query: 418 VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDS 597 V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDI 156 Query: 598 GDGVSHTXPHLRGI 639 G + P + G+ Sbjct: 157 GSDNASVTPVVDGL 170 Score = 35.9 bits (79), Expect = 0.007 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +2 Query: 137 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSNRG 238 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 51.6 bits (118), Expect = 1e-07 Identities = 32/103 (31%), Positives = 48/103 (46%), Gaps = 4/103 (3%) Frame = +1 Query: 340 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 507 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 508 TFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTXPHLRG 636 P +A + ++ LYA G G+V+D G + P L G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.12 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +1 Query: 400 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 543 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schizosaccharomyces pombe|chr 1|||Manual Length = 567 Score = 26.6 bits (56), Expect = 4.5 Identities = 13/29 (44%), Positives = 13/29 (44%) Frame = -2 Query: 648 GSAYPS*MGXGVGDTVAGVQHDTGGTTGR 562 G Y S G G GDTV GG GR Sbjct: 131 GGRYGSSGGRGAGDTVVSRSSGAGGARGR 159 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 6.0 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +1 Query: 442 LLTEAPLNPKANREKMTQIMFETFN 516 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 26.2 bits (55), Expect = 6.0 Identities = 12/35 (34%), Positives = 14/35 (40%) Frame = +2 Query: 521 PPCTSPSKPCSRCTRPVVPPVSCWTPATVSPTPXP 625 PP P + T PV PV+ A P P P Sbjct: 367 PPPVMPQPAAAAVTTPVAAPVAAAAAAVPVPPPAP 401 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 7.9 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -3 Query: 179 TYRSHCLLPTRQLLRRTS*ISEI 111 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,299,570 Number of Sequences: 5004 Number of extensions: 70271 Number of successful extensions: 222 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 186 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 216 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 426466470 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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