Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP01_FL5_B02
(854 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 226 4e-60
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 196 2e-51
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 98 1e-21
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.16
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.21
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.5
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 28 1.5
SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 28 1.9
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 2.6
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 3.4
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 5.9
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 226 bits (552), Expect = 4e-60
Identities = 111/147 (75%), Positives = 130/147 (88%)
Frame = +3
Query: 126 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 305
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 306 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 485
YGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTAQQP
Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQP 124
Query: 486 RLFVGMILILIFAEVLGLYGLIVAIYL 566
RLFV MILILIFAEVLGLYGLIVA+ L
Sbjct: 125 RLFVAMILILIFAEVLGLYGLIVALLL 151
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 196 bits (479), Expect = 2e-51
Identities = 91/147 (61%), Positives = 116/147 (78%)
Frame = +3
Query: 126 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 305
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 306 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 485
YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+ +Q
Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQD 126
Query: 486 RLFVGMILILIFAEVLGLYGLIVAIYL 566
R+FV M+LILIFAEVLGLYGLIV + L
Sbjct: 127 RIFVSMVLILIFAEVLGLYGLIVGLIL 153
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 98.3 bits (234), Expect = 1e-21
Identities = 49/147 (33%), Positives = 81/147 (55%), Gaps = 6/147 (4%)
Frame = +3
Query: 144 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 323
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 324 VLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQP 485
++ + + + PA + Y GF G+ VG L G +GI G + AQ
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 486 RLFVGMILILIFAEVLGLYGLIVAIYL 566
LFV ++++ IF VLGL+GLIV + +
Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.16
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = -2
Query: 544 P*RPNTSAKIRIRIIPTNNLGC*AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 374
P RP ++A ++ PT +VP P++P MP P+A P A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 31.1 bits (67), Expect = 0.21
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +3
Query: 270 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 449
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 450 GDAGVRGT 473
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 1.5
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -2
Query: 475 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 302
A P P S P++PM PAA P P+A AP PL AG AP + A P
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478
Query: 301 AIIPA 287
A PA
Sbjct: 479 APAPA 483
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 28.3 bits (60), Expect = 1.5
Identities = 10/37 (27%), Positives = 20/37 (54%)
Frame = -2
Query: 799 LXXEQPXQKGCTGLEIWENEQGTNVIYKSGRCSETLC 689
L E P ++ C ++ WE+ T+ ++ G+C + C
Sbjct: 12 LSLETPSERTCFCIKCWESVPSTSQVWFGGKCWHSDC 48
>SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 281
Score = 27.9 bits (59), Expect = 1.9
Identities = 17/39 (43%), Positives = 25/39 (64%), Gaps = 3/39 (7%)
Frame = -1
Query: 572 CVQVDGDD-KSV--KTQYFSENKNKNHSDE*PRLLSSTT 465
C++VD +D K + K+QY +EN N N + P L S+TT
Sbjct: 233 CIEVDSEDWKDLVWKSQYATENANTNSINNSP-LSSNTT 270
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 2.6
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +3
Query: 129 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 308
+YGPF+ A+ FS Y +G G + S+ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 309 GLVVAVLIAGAL 344
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.1 bits (57), Expect = 3.4
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +3
Query: 105 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 269
+K+A ++P I F+G ++G + F+ + + ++ G S LI+ S
Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483
Query: 270 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 371
+ P+V++ IIA + + +++ L + + Y +Y
Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 5.9
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 383 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 270
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,603,461
Number of Sequences: 5004
Number of extensions: 77259
Number of successful extensions: 237
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 222
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 233
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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