BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= FWDP01_FL5_B01 (838 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 308 9e-85 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 172 6e-44 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 170 2e-43 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 155 6e-39 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.5 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.3 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.4 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.8 SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 7.6 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 308 bits (755), Expect = 9e-85 Identities = 143/220 (65%), Positives = 167/220 (75%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 268 MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 269 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 448 PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120 Query: 449 RKEAESCDCLQGIPTDTLARRRHRFRYGHPPHLQNQRRVPDRIMNTYSVVPSPKVSDTVV 628 R+EAE+CD LQG G + + PDR+M T+SV P+PK SDTVV Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180 Query: 629 EPYNATLSVHQFS*NPDETYCIDNEALYDICFRTLKLSPP 748 EPYNATLS+HQ N DET+CIDNEAL I TLK+ P Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 172 bits (418), Expect = 6e-44 Identities = 89/222 (40%), Positives = 125/222 (56%), Gaps = 2/222 (0%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 262 MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 263 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 442 YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120 Query: 443 VVRKEAESCDCLQGIPTDTLARRRHRFRYGHPPHLQNQRRVPDRIMNTYSVVPSPKVSDT 622 +R+ A++C LQG +G + + +SV P+P+VS + Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180 Query: 623 VVEPYNATLSVHQFS*NPDETYCIDNEALYDICFRTLKLSPP 748 VVEPYN+ L+ H D T+ +DNE+ YDIC R L + P Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERP 222 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 170 bits (414), Expect = 2e-43 Identities = 93/238 (39%), Positives = 130/238 (54%), Gaps = 18/238 (7%) Frame = +2 Query: 89 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 250 MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 251 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 430 GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120 Query: 431 SVLDVVRKEAESCDCLQGI------------PTDTLARRRHRFRYGHPPHLQNQRRVPDR 574 SVL+ +R+ A++C LQG L R YG +LQ Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQ-------- 172 Query: 575 IMNTYSVVPSPKVSDTVVEPYNATLSVHQFS*NPDETYCIDNEALYDICFRTLKLSPP 748 +SV P+P+VS +VVEPYN+ L+ H N D T+ +DNEA YDIC R L + P Sbjct: 173 ----FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERP 226 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 155 bits (377), Expect = 6e-39 Identities = 80/222 (36%), Positives = 128/222 (57%), Gaps = 3/222 (1%) Frame = +2 Query: 92 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 271 REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 272 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 445 RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+ Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121 Query: 446 VRKEAESCDCLQGIPTDTLARRRHRFRYGHPPHLQNQRRVPDRIMNTYSVVP-SPKVSDT 622 + +EA+ D L+G G + R P +I+ TYSV P S VSD Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181 Query: 623 VVEPYNATLSVHQFS*NPDETYCIDNEALYDICFRTLKLSPP 748 VV+PYN+ L++ + + N D +DN AL I L P Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 2.5 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = +2 Query: 392 AKGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 490 A+GH G ELV + D +RK++E+ L+ P Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 3.3 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 128 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 229 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 4.4 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +2 Query: 323 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 424 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 26.2 bits (55), Expect = 5.8 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = -2 Query: 465 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 328 ++A+ RTTS T+ +PS L P P + S R+ CPK Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 7.6 Identities = 12/44 (27%), Positives = 20/44 (45%) Frame = +2 Query: 344 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 475 RP +F G++ G + E D ++ + + ESCDC Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,964,128 Number of Sequences: 5004 Number of extensions: 57746 Number of successful extensions: 168 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 153 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 412451140 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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