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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP01_FL5_A16
         (849 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g09140.1 68417.m01509 DNA mismatch repair protein MLH1 (MLH1)...   113   2e-25
At4g31880.1 68417.m04531 expressed protein                             34   0.10 
At2g34210.1 68415.m04186 KOW domain-containing transcription fac...    33   0.24 
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID...    31   0.97 
At1g71470.1 68414.m08259 hypothetical protein                          29   3.9  
At5g45095.1 68418.m05532 expressed protein                             28   6.8  
At5g16030.1 68418.m01874 expressed protein                             28   6.8  
At3g17360.1 68416.m02218 kinesin motor protein-related similar t...    28   6.8  

>At4g09140.1 68417.m01509 DNA mismatch repair protein MLH1 (MLH1)
           identical to MLH1 protein [Arabidopsis thaliana]
           GI:3893081
          Length = 737

 Score =  113 bits (271), Expect = 2e-25
 Identities = 68/185 (36%), Positives = 101/185 (54%), Gaps = 8/185 (4%)
 Frame = +3

Query: 33  IHGYITNVNYSNKKGILLLFINHRLVDSQAIRKAVDSVYSTYLPKNSHAFVYLSIELDPK 212
           + G+I+N NY  KK IL+LFIN RLV+  A+++A++ VY+  LPK S  FVY+SI L  +
Sbjct: 266 MEGFISNSNYVAKKTILVLFINDRLVECSALKRAIEIVYAATLPKASKPFVYMSINLPRE 325

Query: 213 NVDVNVHPTKHEVQFLYEEQIVEKIKTCIETKLLGCSSTR------VLYTQARLPGAAQP 374
           +VD+N+HPTK EV  L +E I+E I++ +E KL   + TR      V Y Q+ L      
Sbjct: 326 HVDINIHPTKKEVSLLNQEIIIEMIQSEVEVKLRNANDTRTFQEQKVEYIQSTLTSQKSD 385

Query: 375 RDVADKPT--DAAKTSAAHMVRVNPNVQKIDKFFELEPNKTKQPTGDNASDVVNDVDQQQ 548
             V+ KP+     K     MVR + +         L+P     P   ++  VV    +Q+
Sbjct: 386 SPVSQKPSGQKTQKVPVNKMVRTDSSDPAGRLHAFLQPKPQSLPDKVSSLSVVRSSVRQR 445

Query: 549 KIPSE 563
           + P E
Sbjct: 446 RNPKE 450


>At4g31880.1 68417.m04531 expressed protein
          Length = 873

 Score = 34.3 bits (75), Expect = 0.10
 Identities = 23/89 (25%), Positives = 41/89 (46%)
 Frame = +3

Query: 396 TDAAKTSAAHMVRVNPNVQKIDKFFELEPNKTKQPTGDNASDVVNDVDQQQKIPSEQDLN 575
           TDA K  +      N   Q+ D   + +  K +  TG        D  QQ   P   DLN
Sbjct: 293 TDAPKDESGKSGVSNGVAQQNDSSVDTDSMKKQDDTG------AKDEPQQLDNPRNTDLN 346

Query: 576 NSIVKEPAEDNKVQEKWKQYTEAGQANIT 662
           N+  ++P  +++++EK  + +   QA+++
Sbjct: 347 NTTEEKPDVEHQIEEKENESSSVKQADLS 375


>At2g34210.1 68415.m04186 KOW domain-containing transcription factor
           family protein
          Length = 990

 Score = 33.1 bits (72), Expect = 0.24
 Identities = 24/72 (33%), Positives = 36/72 (50%), Gaps = 3/72 (4%)
 Frame = +3

Query: 384 ADKPTDAAKTSAAHMVR-VNPNVQKIDKFFE--LEPNKTKQPTGDNASDVVNDVDQQQKI 554
           AD P + +     +  R  +P+ + +D+  +  LE   TK    D   D VNDVDQQ  +
Sbjct: 104 ADLPNEDSDHRRQYYQRGFHPHEEDVDELEKRTLERLSTKYAKDDYELDDVNDVDQQALL 163

Query: 555 PSEQDLNNSIVK 590
           PS +D    +VK
Sbjct: 164 PSVRDPKLWLVK 175


>At1g67120.1 68414.m07636 midasin-related similar to Midasin
            (MIDAS-containing protein) (Swiss-Prot:Q12019)
            [Saccharomyces cerevisiae]; similar to Midasin
            (MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
            sapiens]; contains Prosite PS00017: ATP/GTP-binding site
            motif A (P-loop)
          Length = 5336

 Score = 31.1 bits (67), Expect = 0.97
 Identities = 18/81 (22%), Positives = 37/81 (45%)
 Frame = +3

Query: 378  DVADKPTDAAKTSAAHMVRVNPNVQKIDKFFELEPNKTKQPTGDNASDVVNDVDQQQKIP 557
            D  D   +  +T    +  + P+V       ++E +KT++   ++A+       + QK P
Sbjct: 4653 DDTDNLEEKIQTKEEALGGLTPDVDNEQIDDDMEMDKTEEVEKEDANQQEEPCSEDQKHP 4712

Query: 558  SEQDLNNSIVKEPAEDNKVQE 620
             E + +    +EP+E+N   E
Sbjct: 4713 EEGENDQEETQEPSEENMEAE 4733


>At1g71470.1 68414.m08259 hypothetical protein
          Length = 131

 Score = 29.1 bits (62), Expect = 3.9
 Identities = 14/54 (25%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = +3

Query: 477 EPNKTKQPTGDN-ASDVVNDVDQQQKIPSEQDLNNSIVKEPAEDNKVQEKWKQY 635
           +P  +++   D+  S+   D D  +  PS+ + NN +V E   +    E W +Y
Sbjct: 11  KPESSEEELSDSQVSNSSEDDDSMEDEPSDSENNNGVVTETEANGIKDEFWARY 64


>At5g45095.1 68418.m05532 expressed protein 
          Length = 394

 Score = 28.3 bits (60), Expect = 6.8
 Identities = 15/59 (25%), Positives = 28/59 (47%)
 Frame = +3

Query: 123 IRKAVDSVYSTYLPKNSHAFVYLSIELDPKNVDVNVHPTKHEVQFLYEEQIVEKIKTCI 299
           +R ++ ++   YLP     F++++  L    +D N HP    V  L   QI +K+   +
Sbjct: 158 VRVSLFALSGLYLP----VFIFITHRLKHHKIDFNQHPQSSSVHILNWFQISKKVSAIV 212


>At5g16030.1 68418.m01874 expressed protein
          Length = 339

 Score = 28.3 bits (60), Expect = 6.8
 Identities = 22/107 (20%), Positives = 50/107 (46%)
 Frame = +3

Query: 312 LGCSSTRVLYTQARLPGAAQPRDVADKPTDAAKTSAAHMVRVNPNVQKIDKFFELEPNKT 491
           +G  ST  +   +  P  A+   + ++       +    V +N  V+   +  +LE  K 
Sbjct: 201 VGIQSTTSVDLSSGSPSPAKTPPIMERSLKRHVEADDWPVDINLKVKGQQQDVKLE-EKE 259

Query: 492 KQPTGDNASDVVNDVDQQQKIPSEQDLNNSIVKEPAEDNKVQEKWKQ 632
           K+   +   D+ N+ D++++   +QD++    KE  ++ + +EK K+
Sbjct: 260 KE---EEKQDMSNEEDEEEEEEEKQDMSEEDDKEEEDEQEEEEKTKK 303


>At3g17360.1 68416.m02218 kinesin motor protein-related similar to
            KLP2 protein GB:CAA63826 from [Xenopus laevis]
          Length = 2008

 Score = 28.3 bits (60), Expect = 6.8
 Identities = 27/141 (19%), Positives = 57/141 (40%)
 Frame = +3

Query: 240  KHEVQFLYEEQIVEKIKTCIETKLLGCSSTRVLYTQARLPGAAQPRDVADKPTDAAKTSA 419
            K  + +L++++     ++C+    L    T VL TQ  L    +   +     +  K   
Sbjct: 1872 KRHIDYLFKDR-----ESCMSE--LNKKDTDVLATQISLDQLQERVQLLSMQNEMLKNDK 1924

Query: 420  AHMVRVNPNVQKIDKFFELEPNKTKQPTGDNASDVVNDVDQQQKIPSEQDLNNSIVKEPA 599
            ++++R    + +     +   ++  Q T D AS  + D D  +++ + Q L +    E A
Sbjct: 1925 SNLLRKLAELDRTVHNAQASNHRVPQTTKDTASFKLADTDYTKRLENAQKLLSHANNELA 1984

Query: 600  EDNKVQEKWKQYTEAGQANIT 662
            +  K           GQ++ T
Sbjct: 1985 KYRKTSNNHPSTRTQGQSSGT 2005


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,915,104
Number of Sequences: 28952
Number of extensions: 277101
Number of successful extensions: 908
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 889
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 908
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1970388800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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