Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP18_FL5_B12
(855 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein L3|Sch... 233 2e-62
SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3 |Schiz... 233 2e-62
SPBC1683.12 |||nicotinic acid plasma membrane transporter |Schiz... 26 5.9
SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces po... 26 5.9
SPCC777.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 7.8
>SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein
L3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 233 bits (571), Expect = 2e-62
Identities = 108/187 (57%), Positives = 139/187 (74%)
Frame = +3
Query: 159 LVREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCR 338
+VR+ DRPGSK++K+EI+EAVTIIETPPMV VGVVGY+ETP GLR+L TVWAEH+SE+ +
Sbjct: 56 IVRDLDRPGSKMHKREILEAVTIIETPPMVVVGVVGYVETPRGLRSLTTVWAEHLSEEVK 115
Query: 339 RRFYKNWYKCKKKAFTKASXKWQDELGRKSIXKDFXXMIRYCSVVRVIAHTQMKLLKQRQ 518
RRFYKNW+K KKKAFTK + K+ + +SI ++ + +YCSVVRV+AHTQ++ Q
Sbjct: 116 RRFYKNWFKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQIRKTPLAQ 173
Query: 519 KKAHIMEIQLNGGTIEDKVKWATXHLEKPIPVDSVFAQXEMIDCIXXXXXXXXXXXXSRW 698
KKAH+MEIQ+NGG++ DKV+WA H EK + + S F Q EMID I +RW
Sbjct: 174 KKAHLMEIQVNGGSVADKVEWAREHFEKTVDIKSTFEQNEMIDVIGVTRGKGNEGTTARW 233
Query: 699 HTKXLPR 719
TK LPR
Sbjct: 234 GTKRLPR 240
Score = 94.7 bits (225), Expect = 1e-20
Identities = 40/50 (80%), Positives = 44/50 (88%)
Frame = +2
Query: 8 FSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKAGMTH 157
F PRHGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+GYKAGMTH
Sbjct: 6 FEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLGYKAGMTH 55
Score = 74.9 bits (176), Expect = 1e-14
Identities = 34/53 (64%), Positives = 40/53 (75%)
Frame = +1
Query: 697 GTQRXYPXKTHKGLXKVACIGAWHPSRVSFTVXRAGXKGYHXRTEMNXKIYPI 855
GT+R P KTH+GL KVACIGAWHP+ V +TV RAG GY RT++N KIY I
Sbjct: 234 GTKRL-PRKTHRGLRKVACIGAWHPANVQWTVARAGNAGYMHRTQLNSKIYRI 285
>SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 233 bits (570), Expect = 2e-62
Identities = 107/187 (57%), Positives = 139/187 (74%)
Frame = +3
Query: 159 LVREPDRPGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCR 338
+VR+ DRPGSK++K+EI+EAVT+IETPPMV VGVVGY+ETP GLR+L TVWAEH+SE+ +
Sbjct: 56 IVRDLDRPGSKMHKREILEAVTVIETPPMVVVGVVGYVETPRGLRSLTTVWAEHLSEEVK 115
Query: 339 RRFYKNWYKCKKKAFTKASXKWQDELGRKSIXKDFXXMIRYCSVVRVIAHTQMKLLKQRQ 518
RRFYKNW+K KKKAFTK + K+ + +SI ++ + +YCSVVRV+AHTQ++ Q
Sbjct: 116 RRFYKNWFKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQIRKTPLAQ 173
Query: 519 KKAHIMEIQLNGGTIEDKVKWATXHLEKPIPVDSVFAQXEMIDCIXXXXXXXXXXXXSRW 698
KKAH+MEIQ+NGG++ DKV+WA H EK + + S F Q EMID I +RW
Sbjct: 174 KKAHLMEIQVNGGSVADKVEWAREHFEKTVDIKSTFEQNEMIDVIGVTRGKGNEGTTARW 233
Query: 699 HTKXLPR 719
TK LPR
Sbjct: 234 GTKRLPR 240
Score = 94.7 bits (225), Expect = 1e-20
Identities = 40/50 (80%), Positives = 44/50 (88%)
Frame = +2
Query: 8 FSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKAGMTH 157
F PRHGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+GYKAGMTH
Sbjct: 6 FEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLGYKAGMTH 55
Score = 74.9 bits (176), Expect = 1e-14
Identities = 34/53 (64%), Positives = 40/53 (75%)
Frame = +1
Query: 697 GTQRXYPXKTHKGLXKVACIGAWHPSRVSFTVXRAGXKGYHXRTEMNXKIYPI 855
GT+R P KTH+GL KVACIGAWHP+ V +TV RAG GY RT++N KIY I
Sbjct: 234 GTKRL-PRKTHRGLRKVACIGAWHPANVQWTVARAGNAGYMHRTQLNSKIYRI 285
>SPBC1683.12 |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 482
Score = 26.2 bits (55), Expect = 5.9
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 462 CSVVRVIAHTQMKLLKQRQKKAHIMEIQLNGGTIEDK 572
C+VV + T M+ QRQ K I + LNG +++K
Sbjct: 433 CTVVGTLTATAMRFYLQRQNK--IKQEILNGERVDEK 467
>SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1076
Score = 26.2 bits (55), Expect = 5.9
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2
Query: 2 TGFSAPRHGSMGFYPKKRSRRHRGKVK 82
+GFS PR + GF KK+ + R + K
Sbjct: 677 SGFSTPRSQATGFSKKKKDKGERFETK 703
>SPCC777.12c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 238
Score = 25.8 bits (54), Expect = 7.8
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +1
Query: 1 YWIFSTPSWVYGILSQKEVP 60
YW+F +YG LSQK P
Sbjct: 181 YWVFGKALALYGHLSQKYTP 200
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,371,189
Number of Sequences: 5004
Number of extensions: 65576
Number of successful extensions: 149
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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