Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP17_FL5_E02
(858 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 260 1e-70
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 253 2e-68
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 128 8e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 114 1e-26
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.0
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 26 7.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 260 bits (638), Expect = 1e-70
Identities = 114/174 (65%), Positives = 139/174 (79%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVHLVLD 465
+VPR+++VDLEP V+D+VRTG YR LFHP QLITGKEDA+NNYARGHYT+GKE+V V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 627
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPA
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174
Score = 70.5 bits (165), Expect = 3e-13
Identities = 30/44 (68%), Positives = 34/44 (77%)
Frame = +3
Query: 639 TAVVXPYNSILTXHTTLEHSDCAFMVDNEXXYDICRRNLHXXRP 770
T+VV PYNS+LT H TL+ +DC FMVDNE YDICRRNL RP
Sbjct: 179 TSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERP 222
Score = 36.7 bits (81), Expect = 0.004
Identities = 16/30 (53%), Positives = 21/30 (70%)
Frame = +2
Query: 767 PTYTNLNXFIGXIVSSITAFLKIDXXLNLD 856
P+Y NLN I +VSSITA L+ + LN+D
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNVD 251
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 253 bits (620), Expect = 2e-68
Identities = 115/179 (64%), Positives = 141/179 (78%), Gaps = 5/179 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 270
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHP Q++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 HLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 627
VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPA
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178
Score = 73.3 bits (172), Expect = 4e-14
Identities = 31/44 (70%), Positives = 35/44 (79%)
Frame = +3
Query: 639 TAVVXPYNSILTXHTTLEHSDCAFMVDNEXXYDICRRNLHXXRP 770
T+VV PYNS+LT H TL++SDC FMVDNE YDICRRNL RP
Sbjct: 183 TSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERP 226
Score = 36.7 bits (81), Expect = 0.004
Identities = 17/30 (56%), Positives = 20/30 (66%)
Frame = +2
Query: 767 PTYTNLNXFIGXIVSSITAFLKIDXXLNLD 856
PTY NLN I +VSSITA L+ LN+D
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVD 255
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 128 bits (310), Expect = 8e-31
Identities = 64/174 (36%), Positives = 92/174 (52%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVHLVLD 465
+VPRAV VDLEP +D V++G + LF P +I G+ A N +A+GHYT G E+ VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPA 627
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PA
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172
Score = 36.7 bits (81), Expect = 0.004
Identities = 18/50 (36%), Positives = 23/50 (46%)
Frame = +3
Query: 621 PRASGFTAVVXPYNSILTXHTTLEHSDCAFMVDNEXXYDICRRNLHXXRP 770
P VV PYN+ L+ H +E+SD F +DNE I L P
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSP 220
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 114 bits (275), Expect = 1e-26
Identities = 61/174 (35%), Positives = 96/174 (55%), Gaps = 2/174 (1%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKE--DAANNYARGHYTIGKEIVHLVL 462
+PRA+ +DLEP VV+ + + TY L++P ++ K A NN+A G Y+ + I ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYP 624
D I + AD L+GF + H L+ERL+ Y KK ++++P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173
Score = 28.7 bits (61), Expect = 1.1
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = +3
Query: 645 VVXPYNSILTXHTTLEHSDCAFMVDNEXXYDICRRNLHXXRP 770
VV PYNS+L ++D ++DN I LH P
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNP 223
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 6.0
Identities = 21/74 (28%), Positives = 33/74 (44%)
Frame = +2
Query: 68 NSTFKQRKKLKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETI 247
+S+ + S + +S S S+ + T+ ST SSL + ++PS T
Sbjct: 135 SSSLLDPSSVSSAILPSSTSVEVSISSSSLSSSDPLTS-STFSSLSSSTSSSQPSVSSTS 193
Query: 248 LSTLSSARPELAST 289
ST SSA P S+
Sbjct: 194 SSTFSSAAPTSTSS 207
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 25.8 bits (54), Expect = 7.9
Identities = 11/35 (31%), Positives = 20/35 (57%)
Frame = -1
Query: 552 EPRAGTSTEGVEDQESLQTGTLVSELADSVQNQMY 448
+ R G +EG+ E + +LV+ D+V+ +MY
Sbjct: 510 QERVGPESEGIFGDEFFEKLSLVTNALDNVEARMY 544
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,359,772
Number of Sequences: 5004
Number of extensions: 67855
Number of successful extensions: 210
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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