BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BmNP16_T7_B03
(784 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.011
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 34 0.020
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.14
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.19
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 3.0
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 5.3
SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces p... 26 7.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 9.3
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.1 bits (77), Expect = 0.011
Identities = 20/78 (25%), Positives = 24/78 (30%)
Frame = +2
Query: 545 PXARXPXXXXPXPXXXPPAXXXXFXXPPXXPPLXLXPXXPXPXRXXAXXXXPXXXPXCXX 724
P R P P P PP PP PP + P P + P P
Sbjct: 241 PPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNP-AINSTSKPPLPPPSSRV 299
Query: 725 TSXXXXXXPXXXPPPPXP 778
++ PPPP P
Sbjct: 300 SAAALAANKKRPPPPPPP 317
Score = 30.7 bits (66), Expect = 0.25
Identities = 18/64 (28%), Positives = 22/64 (34%), Gaps = 2/64 (3%)
Frame = +1
Query: 484 PXXXXXXPPXXGXXRXXXPPPPXXXSPXXXPAAXLXPPCRXXXXXXP--PPAPXSXPPXP 657
P PP P +P P+A + PP P PPA + PP P
Sbjct: 421 PSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480
Query: 658 XPXP 669
P P
Sbjct: 481 APAP 484
Score = 28.3 bits (60), Expect = 1.3
Identities = 19/70 (27%), Positives = 19/70 (27%), Gaps = 1/70 (1%)
Frame = +1
Query: 451 PXXXXXSPXXXPXXXXXXPPXXGXXRXXXPP-PPXXXSPXXXPAAXLXPPCRXXXXXXPP 627
P P P PP PP PP PA P PP
Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477
Query: 628 PAPXSXPPXP 657
PAP P P
Sbjct: 478 PAPAPAPAAP 487
Score = 25.8 bits (54), Expect = 7.0
Identities = 23/95 (24%), Positives = 26/95 (27%), Gaps = 5/95 (5%)
Frame = +1
Query: 505 PPXXGXXRXXXPPPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAPXSXPPXPXPXPXXXXR 684
P G PPPP P A + P + PPP + P P R
Sbjct: 326 PIGNGSSNSSLPPPPPP--PRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSR 383
Query: 685 RXGT-----PXXXSXLXPXLXXXXRXSXTXAPPPP 774
P P L S T PP P
Sbjct: 384 AVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVP 418
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 34.3 bits (75), Expect = 0.020
Identities = 18/49 (36%), Positives = 19/49 (38%), Gaps = 5/49 (10%)
Frame = +1
Query: 538 PPPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAP-----XSXPPXPXPXP 669
PPPP P PA PP PPP P + PP P P P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 30.7 bits (66), Expect = 0.25
Identities = 14/37 (37%), Positives = 15/37 (40%)
Frame = +1
Query: 559 SPXXXPAAXLXPPCRXXXXXXPPPAPXSXPPXPXPXP 669
SP P A + P PPPAP P P P P
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.5 bits (68), Expect = 0.14
Identities = 20/82 (24%), Positives = 20/82 (24%)
Frame = +2
Query: 533 AXPPPXARXPXXXXPXPXXXPPAXXXXFXXPPXXPPLXLXPXXPXPXRXXAXXXXPXXXP 712
A P P P P PP PP P P P P P P
Sbjct: 1139 APPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAP 1198
Query: 713 XCXXTSXXXXXXPXXXPPPPXP 778
S P PP P
Sbjct: 1199 PVPKPSVGVPPVPPPSTAPPVP 1220
Score = 29.9 bits (64), Expect = 0.43
Identities = 16/65 (24%), Positives = 19/65 (29%)
Frame = +1
Query: 469 SPXXXPXXXXXXPPXXGXXRXXXPPPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAPXSXP 648
+P P P P P P P+ P + P PAP P
Sbjct: 1062 APPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAP 1121
Query: 649 PXPXP 663
P P P
Sbjct: 1122 PVPKP 1126
Score = 28.3 bits (60), Expect = 1.3
Identities = 22/102 (21%), Positives = 26/102 (25%)
Frame = +1
Query: 358 PTXPXXXXXXXXXPXPXXXXXXXAXRXPXXXPXXXXXSPXXXPXXXXXXPPXXGXXRXXX 537
P P P P A P +P P PP
Sbjct: 1064 PPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPV-PKPSVAVPPVPAPSGAPP 1122
Query: 538 PPPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAPXSXPPXPXP 663
P P +P + P + P PAP PP P P
Sbjct: 1123 VPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKP 1164
Score = 28.3 bits (60), Expect = 1.3
Identities = 16/61 (26%), Positives = 19/61 (31%), Gaps = 1/61 (1%)
Frame = +1
Query: 484 PXXXXXXPPXXGXXRXXXP-PPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAPXSXPPXPX 660
P PP P P P P P + P + P P P + PP P
Sbjct: 1162 PKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPT 1221
Query: 661 P 663
P
Sbjct: 1222 P 1222
Score = 27.9 bits (59), Expect = 1.7
Identities = 14/37 (37%), Positives = 15/37 (40%)
Frame = +1
Query: 541 PPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAPXSXPP 651
PPP P P+A L P P PAP S P
Sbjct: 1211 PPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAP 1247
Score = 27.1 bits (57), Expect = 3.0
Identities = 16/61 (26%), Positives = 19/61 (31%), Gaps = 1/61 (1%)
Frame = +1
Query: 484 PXXXXXXPPXXGXXRXXXPPPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAPXS-XPPXPX 660
P PP P PP +P + PP P P P + PP P
Sbjct: 1172 PAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPV 1231
Query: 661 P 663
P
Sbjct: 1232 P 1232
Score = 26.6 bits (56), Expect = 4.0
Identities = 20/86 (23%), Positives = 24/86 (27%), Gaps = 4/86 (4%)
Frame = +3
Query: 534 PSPPPXXXIPXXXXRXRXXXPLPXXXXSRPPPXXXLXXSXPXXXXPXGXSPXXXXXXXXX 713
P+ P P + P +R PP L P P +P
Sbjct: 982 PTSEPPKDHPPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVPSTAP 1041
Query: 714 XVP----XPPXPXXXXLXXXPPPPXP 779
VP PP P PPP P
Sbjct: 1042 PVPIPTSTPPVPKSSSGAPSAPPPVP 1067
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 31.1 bits (67), Expect = 0.19
Identities = 23/83 (27%), Positives = 27/83 (32%), Gaps = 6/83 (7%)
Frame = +1
Query: 544 PPXXXSPXXXPAAXLXPPCRXXXXXX-PPPAPXSXPP-----XPXPXPXXXXRRXGTPXX 705
PP +P P + L PP PPP+P S PP P P + P
Sbjct: 124 PPSAPAPPT-PQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVK 182
Query: 706 XSXLXPXLXXXXRXSXTXAPPPP 774
P L PPPP
Sbjct: 183 SPPSAPSLPSAVPPMPPKVPPPP 205
Score = 29.5 bits (63), Expect = 0.57
Identities = 15/41 (36%), Positives = 16/41 (39%), Gaps = 1/41 (2%)
Frame = +1
Query: 538 PPPPXXXSPXXXPAAXLX-PPCRXXXXXXPPPAPXSXPPXP 657
PP P P PAA + PP PP P PP P
Sbjct: 165 PPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205
Score = 29.1 bits (62), Expect = 0.75
Identities = 23/84 (27%), Positives = 27/84 (32%), Gaps = 2/84 (2%)
Frame = +2
Query: 527 AXAXPPPXA--RXPXXXXPXPXXXPPAXXXXFXXPPXXPPLXLXPXXPXPXRXXAXXXXP 700
A A P P + R P P P PP+ P PP + P P + A P
Sbjct: 127 APAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPP--IPSSLPPPAQPAAPVKSP 184
Query: 701 XXXPXCXXTSXXXXXXPXXXPPPP 772
P P PPPP
Sbjct: 185 ---PSAPSLPSAVPPMPPKVPPPP 205
Score = 27.5 bits (58), Expect = 2.3
Identities = 17/60 (28%), Positives = 20/60 (33%), Gaps = 5/60 (8%)
Frame = +1
Query: 505 PPXXGXXRXXXPPPPXXXSPXXXPAAX-----LXPPCRXXXXXXPPPAPXSXPPXPXPXP 669
PP R PPP +P A L PP + PP+ S P P P
Sbjct: 139 PPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMP 198
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 3.0
Identities = 16/46 (34%), Positives = 17/46 (36%), Gaps = 6/46 (13%)
Frame = +1
Query: 538 PPPPXXXSPXXXPAAXLXPPCRXXXXXX------PPPAPXSXPPXP 657
PPPP P + L P R PPPA S PP P
Sbjct: 240 PPPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPPPPATPSQPPRP 285
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.2 bits (55), Expect = 5.3
Identities = 14/43 (32%), Positives = 16/43 (37%), Gaps = 1/43 (2%)
Frame = +1
Query: 538 PPPPXXXSPXXXPAAXLXPPCRXXXXXXPPPAPXS-XPPXPXP 663
PPPP PA PP + PP A + PP P
Sbjct: 909 PPPPTASMTASAPAIASPPPPKVGETYHPPTASGTRVPPVQQP 951
>SPBC146.07 |prp2|mis11|U2AF large subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 517
Score = 25.8 bits (54), Expect = 7.0
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -1
Query: 319 LRSLISNLSNTCDLTPLPEWSCEQSAW 239
+RS+ L D+TP+ +W ++S W
Sbjct: 109 VRSIEQELEQLRDVTPINQWKRKRSLW 135
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.4 bits (53), Expect = 9.3
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +1
Query: 622 PPPAPXSXPPXPXPXP 669
PPP P S PP P P
Sbjct: 1707 PPPPPMSVPPPPSAPP 1722
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,417,330
Number of Sequences: 5004
Number of extensions: 18329
Number of successful extensions: 134
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 46
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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